Fingerprint and multi-ingredient quantitative analyses for quality evaluation of hawthorn leaves and Guang hawthorn leaves by UPLC–MS

Xu, Baoxin; Yang, Mingyu; Du, Yilong; Zhao, Shengnan; Li, Yanrong; Haifeng, Pan

doi:10.1016/j.bjp.2018.03.005

Cited by 11 publications

(2 citation statements)

References 14 publications

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“…Traditional Chinese medicine (TCM) fingerprinting is frequently utilized in the quality assessment of TCM with complex components 7,8 with high-performance liquid chromatography fingerprint (HPLC-FP) playing a key role. 9,10 However, as the peak separation and response can be influenced by the extraction solvent, elution gradient, and other variables, a super-informative parameter must be given in order to fully characterize the ideal peak.…”

Section: Introductionmentioning

confidence: 99%

Reliability evaluation of traditional Chinese medicine fingerprints combined with qualitative and quantitative analysis and antioxidant activity to comprehensively evaluate the quality of Citri Reticulatae Pericarpium

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Reliability evaluation of traditional Chinese medicine fingerprints combined with qualitative and quantitative analysis and antioxidant activity to comprehensively evaluate the quality of Citri Reticulatae Pericarpium

et al. 2022

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“…Thus, it is difficult to accurately ascertain the internal comprehensive quality of TCM [3]. Therefore, quality control model for multi-component simultaneous determination has emerged as a quite important and indispensable tool for TCM's quality control [4,5].…”

Section: Introductionmentioning

confidence: 99%

Fingerprint and multi-component quantitative analyses for quality evaluation of Rhizoma coptidis steamed with rice wine

Wang¹,

Zeng²,

Lou³

et al. 2021

Trop. J. Pharm Res

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Purpose: To establish a method for the simultaneous determination of multi-components of Rhizoma coptidis steamed with rice wine (RCRW), and to provide a reference for assessing its standard of quality. Method: Chromatographic separation was performed on a high performance liquid chromatography (HPLC) system to determine the characteristic fingerprint of RCRW. The mobile phase consisted of acetonitrile (A) and 0.1 % trifluoroacetic acid (B), with gradients of B as follows: 15 - 20 % from 0 – 30 min; 20 - 25 % from 30 - 50 min; 25 - 35 % for 50 - 60 min, and 35 % for 60 - 70 min. Results: In the multiple reaction monitoring mode, eight components of RCRW were isolated by HPLCphoto-diode array (PDA) method. A fingerprint of the RCRW was established and 8 peaks were calibrated. The method was further validated in terms of linearity (R2 > 0.9993), precision (relative standard deviation, RSD < 1.51 %); repeatability (RSD < 2.98 %) and stability (RSD < 1.93 %). Mean recovery rate ranged from 96.2 to 103.8 %, while RSD values ranged from 0.92 to 2.88 %. Conclusion: These results show that HPLC-PDA method is accurate and feasible, and that they provide a reference for further comprehensive and effective quality control of RCRW.

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Rapid determination of four flavonoids from hawthorn leaves in plasma by ultra‐high performance liquid chromatography‐tandem mass spectrometry and study on its pharmacokinetics

Xu,

Zhou,

et al. 2024

Separation Science Plus

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In this study, a rapid and sensitive ultra‐high performance liquid chromatography‐tandem mass spectrometry method was established for the simultaneous determination of four Vitexin‐4“‐O‐glucoside (VOG), Vitexin‐2”‐O‐rhamnoside (VOR), vitexin (VIT), and hyperoside (HP) in plasma, which are representative flavonoids constituents of hawthorn leaves. The four components were separated by CORTECS UPLC C18 column (100 × 3.0 mm, 1.6 µm), using acetonitrile‐water containing 0.02% formic acid and 2 mmol/L ammonium formate as the mobile phase, the gradient elution flow rate was 0.30 mL/min. The analyte was quantified using an electrospray ionization triple quadrupole mass spectrometer. The analytes and baicalin internal standard were performed at m/z 593.2/413.2 for VOG, m/z 577.2/413.1 for VOR, m/z 431.1/310.9 for VIT, m/z 463.2/300 for HP and m/z 445.1/269 for internal standard. Among the four flavonoids, the correlation coefficient of the standard curve of each component is between 0.9939 and 0.9987. After oral administration of hawthorn leaf extract, VOG and VOR showed similar pharmacokinetic characteristics; VIT was eliminated in the body more slowly than the other three flavonoids and distributed more in the tissues; HP can be quickly absorbed in the blood. The developed analytical method has high sensitivity, precision, and accuracy with no interference from plasma components and has been successfully used in pharmacokinetic studies.

show abstract

Fingerprint and multi-ingredient quantitative analyses for quality evaluation of hawthorn leaves and Guang hawthorn leaves by UPLC–MS

Cited by 11 publications

References 14 publications

Reliability evaluation of traditional Chinese medicine fingerprints combined with qualitative and quantitative analysis and antioxidant activity to comprehensively evaluate the quality of Citri Reticulatae Pericarpium

Reliability evaluation of traditional Chinese medicine fingerprints combined with qualitative and quantitative analysis and antioxidant activity to comprehensively evaluate the quality of Citri Reticulatae Pericarpium

Fingerprint and multi-component quantitative analyses for quality evaluation of Rhizoma coptidis steamed with rice wine

Rapid determination of four flavonoids from hawthorn leaves in plasma by ultra‐high performance liquid chromatography‐tandem mass spectrometry and study on its pharmacokinetics

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