Reliability evaluation of traditional Chinese medicine fingerprints combined with qualitative and quantitative analysis and antioxidant activity to comprehensively evaluate the quality of Citri Reticulatae Pericarpium

Abstract

Comprehensive evaluation of Citri Reticulatae Pericapium quality by HPLC, UV and antioxidant activity.

Evaluating Paeonia Radix Alba quality by multidimensional quantified fingerprinting combined with antioxidant profiling

“…30 The antioxidant activity of a substance can be evaluated by its scavenging of DPPH radicals (the clearance rate, CL). CL was calculated as shown in eqn (16), 24 A i represented the inverse peak area for different concentrations of PRA ( i = 0.02 g mL −1 , 0.05 g mL −1 , 0.1 g mL −1 , 0.15 g mL −1 , 0.2 g mL −1 , 0.25 g mL −1 ), A j represented the inverse peak area of 100% cleared DPPH at PRA concentration j ( j = 0.25 g mL −1 ), and A 0 represented the peak area of the PRA sample without the DPPH mobile phase. A linear regression equation was established using CL as the ordinate and sample concentration as the horizontal coordinate to calculate the 50% inhibitory concentration (IC 50 ).…”

“…The DPPH method was performed as described in recent literature. 24 On-line antioxidant detection was performed using an Agilent 1100 HPLC system (Agilent, USA) and a 655 Liquid chromatograph (HITA-CHI, China). The flow rate of the carrier (ethanol-water, 85 : 15) was 0.4 mL min À1 , and the flow rate of DPPH (0.066 mg mL À1 ) was 0.3 mL min À1 .…”

Overall control of the quality consistency of Citri Retriculatae Pericapium by combining HPLC fingerprint, terahertz time-domain spectroscopy and chemometrics

“…3.2. Reliability evaluation of fingerprint profiles 30 3.2.1. Reliability theory for reference fingerprint profiles (RFP).…”

Quality evaluation methods of chinese medicine based on scientific supervision: recent research progress and prospects

“…2 ). Wang and colleagues used a double-standard evaluation method to establish a fingerprint of CM to evaluate the antioxidant components of Citri Reticulatae Pericarpium, the method used in this article is also applicable to the analysis of other CM, which provides a comprehensive and practical method for the quality control of CM [ 124 ]. Due to the combination of qualitative and quantitative characterization strategies, the double-standard evaluation system provides an effective and reliable model for complex CM systems.…”