Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand

Kongkiatpaiboon, Sumet; Chewchinda, Savita; Vongsak, Boonyadist

doi:10.1016/j.bjp.2018.03.002

Cited by 28 publications

(32 citation statements)

References 12 publications

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“…e PIT chemical compound from LC-MS/MS results demonstrated that PIT contained 4-CQ, 5-CQ, 3,4-CQ, 3,5-CQ, and 4,5-CQ. ese results are in substantial agreement with those of previous reports in that one of the active ingredients in P. indica (L.) is caffeoylquinic acid derivatives [20,23,35].…”

Section: Discussionsupporting

confidence: 92%

“…High fat diet feeding in mice has been widely used as a model for dyslipidemia studies of antidyslipidemia evaluation because it can induce obesity and dyslipidemia. PIT has been used for a health-promoting drink [20] that has the potential to protect hyperglycemia, obesity, and dyslipidemia.…”

Section: Discussionmentioning

confidence: 99%

“…P. indica (Asteraceae) leaves have been used for a healthpromoting drink [20] and in folk medicine, especially in Southeast Asia, including ailand. e plant has various biological activities, such as a diuretic, and antihyperglycemic activities of its methanolic extract [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Pluchea indica (L.) Less. Tea Ameliorates Hyperglycemia, Dyslipidemia, and Obesity in High Fat Diet-Fed Mice

Sirichaiwetchakoon

Lowe

Kupittayanant

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Pluchea indica (L.) Less. (P. indica) tea has been used for a health-promoting drink, especially in Southeast Asia. The effect of P. indica tea (PIT) on amelioration of hyperglycemia; dyslipidemia that was total cholesterol (TC), LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C), and triglyceride (TG); and obesity in high fat diet-induced (HFD) mice was investigated. Oral glucose tolerance test (OGTT) displayed that PIT at 400 and 600 mg/kg orally ameliorated hyperglycemia with a dose-dependent manner compared to the untreated group. Moreover, PIT at these dosages exhibited significantly lower TC, LDL-C, TG, and perigonadal fat weight in HFD treated mice compared to HFD mice (P<0.05) with a dose-dependent manner. In contrast, HDL-C was higher than in the HFD group, but not a significant difference (P>0.05). The PIT chemical analysis results demonstrated that PIT contained total phenolic content (TPC), total flavonoid content (TFC), 4-O-caffeoylquinic acid (4-CQ), 5-O-caffeoylquinic acid (5-CQ), 3,4-O-dicaffeoylquinic acid (3,4-CQ), 3,5-O-dicaffeoylquinic acid (3,5-CQ), 4,5-O-dicaffeoylquinic acid (4,5-CQ), beta-caryophyllene, and gamma-gurjunene that may play an important role in inhibiting hyperlipidemia and hyperglycemia. Also, histological analysis expressed that the mean area and amount of perigonadal fat adipocytes of PIT treated groups were significantly lower and higher than the HFD group (P<0.05), respectively. The toxicity test of PIT at 600 mg/kg/day in mice showed that serum creatinine, alanine transaminase (ALT), alkaline phosphatase (ALP), and complete blood count (CBC) levels of HFD and PIT treated groups were not significantly different compared to the normal control diet group (NCD) (P>0.05). These results suggest that PIT does not become toxic to the kidney, liver, and blood. In conclusion, PIT has the potential to develop into healthy food supplement or medicine for the prevention and treatment of hyperglycemic, hyperlipidemic, and obese patients.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Pluchea indica (L.) Less. Tea Ameliorates Hyperglycemia, Dyslipidemia, and Obesity in High Fat Diet-Fed Mice

Sirichaiwetchakoon

Lowe

Kupittayanant

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…The main active ingredients of the PIT have been investigated by using LC-MS/MS technique. The result showed that the main chemical compositions of the PIT were caffeoylquinic acid derivatives which correlated with Kongkiatpaiboon et al [ 37 ]. Interestingly, caffeoylquinic acid has been reported for its effect on hyperlipidemia, where the enriching of caffeoylquinic acid derivatives in Pandanus tectorius fruit extract moderated hyperlipidemia and improved the liver lipid profile in hamsters fed high fat diet and these effects may be caused by increasing the expression of PPARα and its downstream genes and by upregulation of LPL and AMPK activities [ 38 ].…”

Section: Discussionsupporting

confidence: 79%

“…Furthermore, our data are consistent with those of Hsu et al that flavonoids and phenolic acids caused 3T3-L1 cell cycle arrest in the G1 phase that may play a role in the control adipogenesis of 3T3-L1 cell and might have the further implication of in vivo antiobesity effects [ 41 ]. Apart from this, the previous study reported that quercetin, which is the one of active ingredients in P. indica (L.) [ 37 ], could inhibit adipogenesis in 3T3-L1 adipocyte cell [ 42 ]. Moreover, quercetin was reported to have the ability to improve hypertriglyceridemia, alleviate hypercholesterolemia, and elevate HDL-cholesterol in db/db mice by decreasing the expression of peroxisome proliferator-activated receptor- α ( PPAR-α ) and sterol regulatory element-binding protein-1c ( SREBP-1c ) and reducing acetyl-CoA carboxylase (ACC) activity [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity

Sirichaiwetchakoon

Lowe

Thumanu

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Obesity and hyperlipidemia are a major problem in the world. Pluchea indica (L.) Less. tea (PIT) is a beverage that has various indications. This study focused on the effect of the PIT on inhibiting adipogenesis of 3T3-L1 cells and pancreatic lipase enzyme activity. The viability of 3T3-L1 cells was not significantly decreased after exposure to 200 to 1000 μg mL−1 PIT compared to controls (p > 0.05). The PIT at 750 to 1000 μg mL−1 exhibited a significantly reduced lipid accumulation compared to the control (p < 0.05). The inhibitory effects of the PIT at 250 to 1000 μg mL−1 on lipase activity were significantly increased compared to control (p < 0.05). The FTIR results showed that the integrated areas of lipids, proteins, nucleic acids, glycogen, and carbohydrates of the PIT-treated 3T3-L1 adipocytes were significantly lower than the untreated 3T3-L1 adipocytes (p < 0.05). These findings may indicate that the PIT is not only capable of inhibiting lipids and carbohydrate accumulation in adipocytes but also has a potential to inhibit pancreatic lipase activity. So, the PIT may be further developed to the novel lipid-lowering herbal supplement for the management of overweight or obesity.

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Four almost unexplored species of Brazilian Connarus (Connaraceae): Chemical composition by ESI‐QTof‐MS/MS–GNPS and a pharmacologic potential

Paim

Santos

Toledo

et al. 2021

Phytochemical Analysis

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Introduction Species of Connaraceae are globally used in traditional medicines. However, several of these have not been studied regarding their chemical composition, and some are even at risk of extinction without proper studies. Therefore, the chemical composition and pharmacological potential of Connarus blanchetii Planch., Connarus nodosus Baker, Connarus regnellii G. Schellenb., and Connarus suberosus Planch., which were previously unknown, were analyzed. Objective This work aims to investigate the pharmacological potential of these four Connarus species. The chemical composition of different extracts was determined by high‐resolution mass spectrometry (HRMS), with subsequent analysis by the GNPS platform and competitive fragmentation modeling (CFM). Materials and methods Leaf extracts (C. blanchetii, C. nodosus, C. regnellii, and C. suberosus) and bark extracts (C. regnellii and C. suberosus) were obtained by decoction, infusion, and maceration. LC/HRMS data were submitted to the GNPS platform and evaluated using CFM in order to confirm the structures. Results The HRMS–GNPS/CFM analysis indicated the presence of 23 compounds that were mainly identified as phenolic derivatives from quercetin and myricetin, of which 21 are unedited in the Connarus genus. Thus, from the analyses performed, we can identify different compounds with pharmacological potential, as well as the most suitable forms of extraction. Conclusion Using HRMS–GNPS/CFM, 21 unpublished compounds were identified in the studied species. Therefore, our combination of data analysis techniques can be used to determine their chemical composition.

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Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand

Cited by 28 publications

References 12 publications

Pluchea indica (L.) Less. Tea Ameliorates Hyperglycemia, Dyslipidemia, and Obesity in High Fat Diet-Fed Mice

Pluchea indica (L.) Less. Tea Ameliorates Hyperglycemia, Dyslipidemia, and Obesity in High Fat Diet-Fed Mice

The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity

Four almost unexplored species of Brazilian Connarus (Connaraceae): Chemical composition by ESI‐QTof‐MS/MS–GNPS and a pharmacologic potential

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