Qualitative and quantitative analysis of the phenolic content of Connarus var. angustifolius , Cecropia obtusa , Cecropia palmata and Mansoa alliacea based on HPLC-DAD and UHPLC-ESI-MS/MS

Pires, Fernanda Brum; Dolwitsch, Carolina Bolssoni; Prá, Valéria Dal; Faccin, Henrique; Monego, Debora; Carvalho, Leandro Machado de; Viana, Carine; Lameira, O. A.; Lima, Fernanda O.; Bressan, Lucas Paines; Rosa, Marcelo Barcellos da

doi:10.1016/j.bjp.2017.03.004

Cited by 20 publications

(16 citation statements)

References 30 publications

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“…Generally, the phenolic compounds separation is carried out by gradient elution using binary systems comprising an aqueous component and a less polar organic solvent such as methanol or acetonitrile. Furthermore, with the aim to control pH in order to control the charge of the molecule, acids such as formic [1,33,77], acetic [2,54], or phosphoric [28] are normally incorporated in low percentages, between 0.005% and 0.5% (v/v) in the aqueous phase or even in both phases. Despite not being frequent, because silica-based columns can be irreversibly damaged at very low pH, some authors use higher percentages of acid (10%) [32].…”

Section: Chromatographic and Mass Spectrometry Conditionsmentioning

confidence: 99%

“…According to Naczk and Shahidi [27], the extraction of phenolic compounds in plants is influenced by several factors. For example, some phenolic compounds are very photosensitive, as a result, rapid extraction methods are necessary to avoid the degradation of them [28]. Liquid-liquid extraction (LLE) and solid-liquid extraction (SLE) followed by a stage of concentration and purification are the most widely used methods to make a selective extraction of phenolic compounds from various matrices [2,3,[29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

“…To overcome this problem, in recent years, the use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been increasing in order to characterize the polyphenol-rich extracts. In addition, LC-MS/MS was able to achieve noise reduction and sensitivity improvements by exploiting multiple reaction monitoring (MRM) scan mode [1,28,29,[33][34][35][36][37][38]. Besides, in the last years, high-resolution LC-MS and LC-MS/MS approaches coupled with multivariate statistics have been widely used to realize the so-called "metabolomic profiling" of plant foods for human nutrition.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

et al. 2020

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In recent years, the consumption of polyphenols has been increasing, largely due to its beneficial effects on health. They are present in a wide variety of foods, but their extraction and characterization are complicated since they are mostly in complex matrices. For this reason, the use of selective, sensitive, and versatile analytical techniques such as liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) is necessary. In this review, the most relevant studies of the last years regarding the analysis of polyphenols in different matrices by comprehensive LC–MS/MS are discussed. Relevant steps such as extraction, sample purification, and chromatographic analysis methods are emphasized. In particular, the following methodological aspects are discussed: (a) the proper selection of the extraction technique, (b) the extraction and elution solvents, (c) the purification step, (d) the selection of both stationary and mobile phases for the chromatographic separation of compounds, and (e) the different conditions for mass spectrometry. Overall, this review presents the data from the most recent studies, in a comprehensive way, thus providing and simplifying the information of the great variety of works that exist in the literature on this wide topic.

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Section: Chromatographic and Mass Spectrometry Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

et al. 2020

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“…These include the Polish blue‐berried honeysuckle, different peach cultivars, and selected medicinal plants ( Connarus var. angustifolius , Cecropia obtusa , Cecropia palmata , and Mansoa alliacea ) evaluated by Pires et al…”

Section: Discussionmentioning

confidence: 99%

Angiotensin‐1‐converting enzyme inhibition, antioxidant activity, and modulation of cerebral Na+/K+ ATPase by free phenolics of African locust bean (Parkia biglobosa)

Komolafe

Akinmoladun

Komolafe

et al. 2017

Health Science Reports

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Aims: To investigate the antioxidant activities and effects of free phenols (FPPB) and bound phenols (BPPB) of Parkia biglobosa leaves on some enzymes of neuro-cardiovascular relevance.Methods and Results: HPLC-DAD fingerprinting of FPPB and BPPB, and the antihemolytic, radical (1,1-diphenyl-2 picrylhydrazyl, DPPH; 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), ABTS) scavenging and ferric reducing antioxidant properties of extracts, were assessed. In addition, the effects of the phenolics on angiotensin-1-converting enzyme (ACE), cerebral acetylcholinesterase/butyrylcholinesterase (AChE/BuChE), and Na + /K + ATPase were determined in vitro. FPPB was more potent than BPPB in terms of ABTS (EC 50 :4.06 ± 0.3 vs 24.07 ± 2.1 μg/mL) and DPPH (EC 50 :3.82 ± 0.2 vs 10.22 ± 0.1 μg/mL) radicals scavenged, respectively. The free phenolic extract was a better DPPH . scavenger than ascorbic acid (EC 50 = 12.58 ± 0.4 μg/mL; DPPH reference) and compared well withTrolox (EC 50 :4.44 ± 0.08 μg/mL; ABTS reference). The anti-hemolytic effect of FPPB (36%) and BPPB (53%) was highest at 15 μg/mL but lower than that recorded for ascorbic acid (67% at 10 μg/mL). Even though FPPB (IC 50 = 15.35 ± 4.0 μg/mL) and BPPB (IC 50 = 46.85 ± 3.3 μg/ mL) showed considerably lower ACE-inhibitory effect than ramipril (IC 50 :0.173 ± 0.04 μg/mL), both extracts demonstrated dose-dependent, significant (p < 0.01/p < 0.05) inhibition of the enzyme. FPPB increased cerebral Na+/K+ATPase activity but neither phenolic extract affects cerebral AChE/BuChE activities. HPLC-DAD revealed catechin, caffeic acid, and quercetin, respectively, as the major phenolics (mg/g) in FPPB (29.85, 30.29, and 17.10) and BPPB (32.70, 30.51, and 19.25). Conclusion:The effects of P biglobosa on ACE and cerebral ATPase are related to its constituent phenolics. ACE inhibition could be an important mechanism underlying the documented hypotensive effect of the plant. ----------------------------------------------This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…Supercritical carbon dioxide extracts of Kigelia have shown potent anti-inflammatory action when compared with indomethacin and by inhibition of cyclooxygenase-2 against human neutrophils [35]. M. alliacea root and stem extracts have shown anti-inflammatory activities by inhibiting prostaglandin synthesis [36].…”

Section: Analgesic Activitymentioning

confidence: 99%

PHYTOCHEMICAL CONSTITUENTS AND PHARMACOLOGICAL ACTIVITIES OF Kigelia africana AND Mansoa alliacea - A COMPREHENSIVE REVIEW

Venkataraman

Srilakshmi

2018

Asian J Pharm Clin Res

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Context:The focus of the present review is to compile the different phytochemical constituents and pharmacological activities of bignoniaceous plants, namely Kigelia africana and Mansoa alliacea.Objective: Medicinal plants are routinely used in clinical practice for the treatment of various diseases of human beings over thousands of years across the world. In rural areas of the developing countries, they are still used in practice due to the easy availability of the medication. The main objective of this work is to explore the phytochemical studies and biological activities of plants belonging to Bignoniaceae family. Methods:The present study also discusses about the different phytochemical constituents of K. africana and M. alliacea that were isolated and characterized using various analytical methods. Different extracts of these plants were subjected to different in vitro anticancer, analgesic, antimicrobial, and antimalarial activities using earlier reported methods were also discussed.Results: K. africana is commonly referred to as sausage or cucumber tree due to its huge sausage or cucumber-like fruit. These plants find application as traditional medicine for treating several ailments such as malignant tumor, ulcer, aging, and malaria among others. It is conjointly used for the treatment of reproductive organ infections, renal ailments, dizziness, epilepsy, sickle cell disease, depression, metabolism disorders, skin grievance, leprosy, impetigo, helminthic infections, athlete's foot, tumors, etc. These plant extracts have also been used as a cosmetic by enhancing the collagen action and thus exhibit antiaging properties. Conclusion:The present study attempts to provide collective information on various phytochemical constituents and pharmacological actions of K. africana and M. alliacea belonging to Bignoniaceae. This review in its present form is believed to help the researchers to provide adequate information about the chemical constituents and biological uses of these plants.

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Qualitative and quantitative analysis of the phenolic content of Connarus var. angustifolius , Cecropia obtusa , Cecropia palmata and Mansoa alliacea based on HPLC-DAD and UHPLC-ESI-MS/MS

Cited by 20 publications

References 30 publications

Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

Angiotensin‐1‐converting enzyme inhibition, antioxidant activity, and modulation of cerebral Na+/K+ ATPase by free phenolics of African locust bean (Parkia biglobosa)

PHYTOCHEMICAL CONSTITUENTS AND PHARMACOLOGICAL ACTIVITIES OF Kigelia africana AND Mansoa alliacea - A COMPREHENSIVE REVIEW

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