Euphorbia umbellata bark extracts – an in vitro cytotoxic study

Kanunfre, Carla Cristine; Leffers, Talissa; Cruz, Luiza Stolz; Luz, Lívia Eidam Camargo; Crisma, Amanda Rabello; Wang, Mei; Avula, Bharathi; Khan, Ikhlas A.; Beltrame, Flávio Luís

doi:10.1016/j.bjp.2016.11.002

Cited by 13 publications

(13 citation statements)

References 39 publications

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“…This effect has been observed for the hexane extract of the latex from E. umbellata and other matrices obtained from Euphorbia species, against leukemia cell lineages. [10,12,14,25,26] The cell cycle analysis demonstrated that the treatment with Dcm increased S and G2/M population (compared with the negative control), as observed to doxorubicin ( Figure 4). Similar data already demonstrated the same effect of doxorubicin in HL-60 cell and some lymphoma cell line cycle, showing an increase of S and G2/M phase cell population, which appears to be related with the upregulation of cyclin B1 and cyclin A2, and expression of topoisomerase IIα.…”

Section: Cytotoxic Assaymentioning

confidence: 68%

“…It is already established in literature the influence of terpenes on cytotoxic activity, especially diterpenes, which are present in Dcm and Eth. [15] Intermediate polarity fractions were more cytotoxic to tumoral cells, including leukemia cell lines; [12,22,23] this may be a result of the chemical affinity of terpenes (di-and triterpenes) with such solvents. [15] Therefore, some mechanisms were evaluated for these two fractions against HL-60 lineage cells trying to increase the Figure 3).…”

Section: Cytotoxic Assaymentioning

confidence: 99%

“…[26] On the other hand, Luz et al [14] described that hexane extract from the latex of E. umbellata promoted cell cycle arrest in phase G0/G1 for Jurkat cells, and a similar result was obtained for Jurkat cells treated with chloroform extract from E. umbellata barks and K-562 cells treated with E. umbellata aerial parts extracts. [10,12] Probably these changes in the cycle arrest can be related with the concentration of the compounds in the vegetal matrix used in these experiments. [26] Other mechanisms studied include the analysis of mitochondrial transmembrane potential and caspases activation.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

“…All the fractions were analyzed by gas chromatography-mass spectrometry (GC/MS) as described by Kanunfre et al [12] Wiley and National Institute of Standards and Technology (NIST) library (version 2.0) were used to assist in compound identification. Further identification was based on the relative retention times compared with the reference standards and the literature values.…”

Section: Chemical Analysismentioning

confidence: 99%

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Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Cruz

Kanunfre

Andrade

et al. 2020

Chemistry & Biodiversity

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The current study was carried out by a bioguided fractionation of a hexane extract of the latex of Euphorbia umbellata against leukemic cells. Samples were analyzed by NMR, GC/MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC/MS analysis. MTT reduction assay demonstrated that HL‐60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices were higher than 22.44. Bioguided‐fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.

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Section: Cytotoxic Assaymentioning

confidence: 68%

Section: Cytotoxic Assaymentioning

confidence: 99%

Section: Cytotoxic Assaymentioning

confidence: 99%

Section: Chemical Analysismentioning

confidence: 99%

See 2 more Smart Citations

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Cruz

Kanunfre

Andrade

et al. 2020

Chemistry & Biodiversity

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“…6 It is known for its high medicinal value and have strong anti-plasmodial, antibacterial, antifungal activity. 7,8 Extracts of S. glauca have been used for treatment of gastrointestinal disorders. 9 Thus, in order to deeply comprehend, we evaluated S. glauca leaf extract for preliminary phytochemical screening, total phenolic content, and in vitro antioxidant potential was analyzed by ferric ion reducing antioxidant power assay, phosphomolybdenum assay, DPPH assay and in vitro anticancer activity was evaluated on T-24 bladder cancer cells by MTT assay.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Puranik¹,

Ghagane²,

Nerli³

et al. 2017

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Objective: Screening of preliminary phytochemicals, evaluation of in vitro antioxidant and in vitro anticancer activities of Simarouba glauca leaf extracts on T-24 Bladder cancer cell line. Materials and Methods: Herbal extraction was carried out by Soxhlet method using chloroform, ethylacetate, methanol, ethanol, aqueous and hydroalcohol. Phytochemical investigation was done using biochemical tests. Total phenolic content was estimated by Folin-Ciocalteu reagent (FCR) method. Antioxidant potential of leaf extracts was analyzed by Ferric ion reducing antioxidant power (FRAP) assay, Phosphomolybdenum (PM) assay and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. In vitro anticancer activity on T-24 bladder cancer cell line was assessed by MTT assay. Statistical analysis used: Statistical analysis of data was performed by analysis of variance (one-way ANOVA) and level of statistical significance between groups was carried out using GraphPad Prism version 5.0 for Windows (GraphPad Software, San Diego, CA, USA). Results: Phytochemical analysis revealed the presence of rich secondary metabolite present in all the solvent extracts. Hydroalcoholic extract showed highest presence of phenolic content (92.38±0.29 mg/g) GAE. Ethanol and methanol extract showed highest antioxidant capacity in DPPH, FRAP and PM assay as compared to the other extracts based on the test performed. The results confirmed that ethanol extract significantly (p<0.05) inhibited T-24 cell line with IC 50 value (533.55±25.02 µg/mL) as compared to standard drug doxorubicin (0.16µM/mL). Conclusions: The results of the present findings strengthen the potential property of Simarouba glauca as a resource for the discovery of novel antioxidant and anticancer agents.Key words: Antioxidant, Anticancer, Bladder Cancer, Phytochemical, Simarouba glauca. Key Messages: The use of medicinal plants have been practiced to treat cancer aliments across the world. Our study demonstrates the implication of herbal extracts to inhibit cancer cell lines at in vitro levels. However, the further approaches at in vivo level using lead compounds will give core insights of S. glauca extracts on Bladder cancer.

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Bioactive Secondary Plant Metabolites from Euphorbia umbellata (PAX) BRUYNS (Euphorbiaceae)

Oliveira

Souza

Fontana

et al. 2022

Chemistry & Biodiversity

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The species Euphorbia umbellata has been used to treat inflammatory diseases, cancer, and ulcers. Biological activities reported in the literature, including antiproliferative, cytotoxic and anti‐inflammatory, are attributed to the chemical constituents present in its composition as terpenes and polyphenolic compounds. The most recurrently verified metabolites in the Euphorbiaceae family plant species are terpenes, of which euphol is a major constituent with broadly reported cytotoxic, antinociceptive and anti‐inflammatory effects; it frequently appears in various extracts obtained from the plant. Euphol has a documented inhibitory effect on neutrophil chemotaxis and can modulate the complement system. Since complement system activation is intimately intertwined with autoimmune and inflammatory diseases, tumor growth promotion and metastasis, plant metabolites from Euphorbia umbellata might influence the outcomes of inflammatory processes. We believe that this is the first review presenting the current knowledge on Euphorbia umbellata secondary metabolites and their biological activities.

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Euphorbia umbellata bark extracts – an in vitro cytotoxic study

Cited by 13 publications

References 39 publications

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Bioactive Secondary Plant Metabolites from Euphorbia umbellata (PAX) BRUYNS (Euphorbiaceae)

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