Inhibition of cytochrome P450 3A enzyme by Millettia aboensis : its effect on the pharmacokinetic properties of efavirenz and nevirapine

Nduka, Sunday Odunke; Okonta, Mathew Jegbefume; Ajaghaku, Daniel Lotanna; Amorha, Kosisochi Chinwendu; Ukwe, Chinwe Victoria

doi:10.1016/j.bjp.2016.10.008

Cited by 6 publications

(2 citation statements)

References 17 publications

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“…Blood samples were collected eight different time-points from the tail vein at 0, 0. 5, 1, 2, 4, 6, 8 and 24 h after administration, and loaded into new clean tubes containing EDTA as previously described 32 .…”

Section: Safety Evaluation Of Hcfp In Healthy Volunteers Although Hmentioning

confidence: 99%

Safety and immunostimulatory activity of the dietary supplement Houttuynia cordata Thunb. fermentation product in healthy volunteers

Woranam¹,

Prompipak²,

Mootsikapun³

et al. 2021

Preprint

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Houttuynia cordata Thunb. fermentation product (HCFP) is widely used in Thailand as a dietary supplement for immune support with no experimental verification. The aim of this study was to investigate the safety and immunomodulation of HCFP in healthy adult volunteers. The effect of HCFP on antiretroviral drugs (TDF and EFV) was also evaluated in Sprague Dawley rats. The basic characteristics and blood chemistry of ten healthy volunteers did not show any significant differences between before and after 4 weeks of intervention with a daily intake of HCFP, and no major adverse event was observed. However, the LDL-c level was significantly decreased after 4 weeks of intervention. Immunomodulation assay revealed that the percentage of neutrophil was significantly increased after 8 weeks of intervention in 30 healthy volunteers. Meanwhile, the mean of CD4+ and CD8+ T-cell ratio was not significantly increased after 8 weeks of intervention. In addition, the plasma concentrations of EFV and TDF in Sprague Dawley rats showed no significant difference between single drug group and combination with HCFP group, suggesting that HCFP has no effect on the plasma level of antiretroviral drugs.

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Section: Safety Evaluation Of Hcfp In Healthy Volunteers Although Hmentioning

confidence: 99%

Safety and immunostimulatory activity of the dietary supplement Houttuynia cordata Thunb. fermentation product in healthy volunteers

Woranam¹,

Prompipak²,

Mootsikapun³

et al. 2021

Preprint

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“…The CYP3A enzyme converts ERY into N-desmethylerythromycin and formaldehyde; therefore, the activity of CYP3A is proportional to the amount of formaldehyde formed. Nduka et al [ 14 , 15 ] used this principle to study the effects of Aframomum melegueta , Denniettia , and Millettia aboensis extracts on CYP3A activity in intestinal and liver microsomes. The cytochrome P450 enzymes in liver microsomes of poultry (turkey, duck, quail, and chicken) that are orthologous to cytochrome P450 enzymes in human and mouse liver microsomes are CYP1A1/2, CYP2A6, and CYP3A4 [ 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Erythromycin and Clarithromycin Metabolites Formed in Chicken Liver Microsomes Using Liquid Chromatography–High-Resolution Mass Spectrometry

Wang

Nam

Kim

et al. 2021

Foods

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Nontargeted analysis can be used for the rapid screening and confirmatory analysis of veterinary drugs and their metabolites, which are important for the comprehensive safety evaluation of animal-derived foods. Here, a novel nontargeted screening approach based on liquid chromatography coupled with electrospray ionization–high-resolution mass spectrometry (LC/ESI–HR-MS) was developed to determine erythromycin, clarithromycin, and their metabolites in chicken liver microsomes. Erythromycin and clarithromycin were incubated in vitro in the presence of NADPH for 60 min to generate metabolites in chicken liver microsomes. After the incubation, the supernatant was extracted using ultrasonic shaking, orbital shaking, and centrifugation before analysis using LC/ESI-HR-MS in positive ion mode on an Agilent Eclipse Plus C18 column (100 mm × 2.1 mm; i.d. 3.5 µm) with 0.1 percent formic acid-water and acetonitrile as the mobile phases for gradient elution at 0.4 mL/min. The results show that erythromycin can produce N-desmethyl-erythromycin A in chicken liver microsomes, but clarithromycin cannot produce N-desmethyl-clarithromycin in chicken liver microsomes. The N-desmethyl-erythromycin A and N-desmethyl-clarithromycin were tentatively identified in chicken liver microsomes using the established quick analytic method, which will provide a theoretical foundation for future research on pharmacokinetics and drug elimination in poultry.

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In vitro and in vivo cytochrome P450 3A enzyme inhibition by Aframomum melegueta and Denniettia tripetala extracts

Nduka

Okonta

Ajaghaku

et al. 2017

Asian Pacific Journal of Tropical Medicine

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Inhibition of cytochrome P450 3A enzyme by Millettia aboensis : its effect on the pharmacokinetic properties of efavirenz and nevirapine

Cited by 6 publications

References 17 publications

Safety and immunostimulatory activity of the dietary supplement Houttuynia cordata Thunb. fermentation product in healthy volunteers

Safety and immunostimulatory activity of the dietary supplement Houttuynia cordata Thunb. fermentation product in healthy volunteers

Identification of Erythromycin and Clarithromycin Metabolites Formed in Chicken Liver Microsomes Using Liquid Chromatography–High-Resolution Mass Spectrometry

In vitro and in vivo cytochrome P450 3A enzyme inhibition by Aframomum melegueta and Denniettia tripetala extracts

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