Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines

Thomas, Anvy Susan; Saravanakumar, Rupachandra; Gupta, Pratiksha V.

doi:10.1016/j.bjp.2016.08.003

Cited by 14 publications

(3 citation statements)

References 18 publications

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“…The cytotoxicity was induced in a concentration dependent manner. Similar results have been observed in the protein extract of Morinda pubescens, which shows the maximum cell viability of the Vero cells at a concentration of 100µg/ml (Thomas et al, 2017). Another protein known as Galanthus nivalis agglutinin showed a minimal inhibitory effect on murine and human cancer cell lines when tested for cytotoxicity (Sandström et al, 2004).…”

Section: Discussionsupporting

confidence: 78%

Anti HCV activity and expression inhibition of HCC markers by protein extract from Iberis gibraltarica

et al. 2024

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Hepatitis C virus infection (HCV) is the foremost reason of progressive hepatic fibrosis and cirrhosis, with an elevated risk of hepatocellular carcinoma (HCC) development. Medicinal plants have been used for human health benefits for several years, but their therapeutic potential needs to be explored. The main objective of this study was to figure out the in vitro antiviral and anticancer characteristics of total crude protein of Iberis gibraltarica against HCV and HCC. Total crude protein of Iberis gibraltarica was isolated and quantified. The level of cytotoxicity was measured against the HepG2 cell line and it shows no significant cytotoxicity at the concentration of 504µg/ml. The anti-HCV effect was determined by absolute quantification via real time RT-PCR method and viral titer was reduced up to 66% in a dose dependent manner against the total protein of Iberis gibraltarica. The anticancer potential of Iberis gibraltarica was also examined through mRNA expression studies of AFP and GPC3 genes against the total protein of Iberis gibraltarica-treated HepG2 cells. The results show up to 90% of the down-regulation expression of AFP and GPC3. The obtained results indicate the therapeutic potential of total protein of Iberis gibraltarica against HCV and hepatocellular carcinoma in vitro.

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Section: Discussionsupporting

confidence: 78%

Anti HCV activity and expression inhibition of HCC markers by protein extract from Iberis gibraltarica

et al. 2024

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“…In the observed pattern of seaweed residual samples (Figure S1A,B), shoulders and low intensity resonances appear in the range 2540-2150 cm −1 , attributed to the stretching modes of the C≡C and C=C moieties. In the range from 1740 to 1388 cm −1 , strong features arose verifying the presence of COOH (C=O) and amino acids (N-H) [27,28]. The peaks between 1082 and 1020 cm −1 were due to the C-O bond stretching mode of polysaccharides and the features from 879 to 790 cm −1 were attributed to C=C stretching vibrations [29].…”

Section: Ft-ir Spectra Of Marine-derived Materialsmentioning

confidence: 99%

Chemical and Biological Profiling of Fish and Seaweed Residues to Be Applied for Plant Fertilization

Maroulis,

Matsia,

Lazopoulos

et al. 2023

Agronomy

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Brown algae and fish waste contain high-value compounds with potentially beneficial effects on plant growth. Several commercial fertilizer products are currently available, but the characteristics of the materials are usually not well-described. Fish and seaweed residues originating from the Norwegian coast are available, after industrial processing, which may be combined into complete fertilizers exerting additional effects on crop plants (biostimulants). In this study, raw samples of fish and seaweed residues were investigated using ecofriendly technologies (drying, leaching), targeting search and isolation of potential biostimulants, followed by physicochemical characterization (elemental analysis, UV–visible, FT-IR, ICP-MS, ICP-OES, electrical conductivity, pH, etc.). Organic solvent extractions were employed to determine the available mineral content, micro- and macro-nutrients, antioxidant compounds, and amino acid content by chemical hydrolysis. The in vitro biotoxicity profile (cell viability, morphology, migration) of the generated extracts was also perused, employing Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) along with sensitive neuronal eukaryotic cell lines N2a58 and SH-SY5Y, to assess their time- and concentration-dependent efficacy as antimicrobials and agents counteracting oxidative stress. The analytical composition of all raw materials showed that they contain important nutrients (K, P, Ca, N) as well as organic compounds and amino acids (Gly, Asp, Glu, Leu, Phe) capable of acting as plant biostimulants. Concurrently, the inherently high conductivity values and salt content necessitated leaching processes, which result in Na+ and K+ decreasing by more than ~60% and justifying further their use in soil treatment formulations. The aforementioned results and assertions, combined with physical measurements (pH, electrical conductivity, etc.) on naturally occurring and dried samples as well as green solvent extracts, formulated a physicochemical profile reflecting well-defined inorganic–organic species that might function as biostimulants. The collective physicochemical and biological properties support the notion that appropriate mixtures of marine organism residues may be efficient fertilizers for crop plants and concurrently possess biostimulant characteristics.

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“…One novel peptide called BG-4 was isolated, purified, and characterized from Momordica charantia plant has shown activity against HCT-116 and HT-29 human colon cancer cells as shown by trypsin inhibitor assay (Dia & Krishnan, 2016). Also, another protein extracted from the leaves of Morinda pubescens has shown cytotoxic activity against A549 human cancer cell line (Thomas et al, 2017). In another study, proteins extracted from Gynura procumbens showed anti-cancer activity in breast cancer cell line, MDA-MB-231 as shown by lactate dehydrogenase (LDH) cytotoxicity assay (Hew et al,2013).…”

Section: Herbal Medicine In Cancer Therapymentioning

confidence: 99%

Proteomic Evaluation and Cytotoxicity of Red Maple (Acer rubrum) Leaves

Alshammari¹

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Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines

Cited by 14 publications

References 18 publications

Anti HCV activity and expression inhibition of HCC markers by protein extract from Iberis gibraltarica

Anti HCV activity and expression inhibition of HCC markers by protein extract from Iberis gibraltarica

Chemical and Biological Profiling of Fish and Seaweed Residues to Be Applied for Plant Fertilization

Proteomic Evaluation and Cytotoxicity of Red Maple (Acer rubrum) Leaves

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