A validated and densitometric HPTLC method for the simultaneous quantification of reserpine and ajmalicine in Rauvolfia serpentina and Rauvolfia tetraphylla

Abstract

Please cite this article in press as: Pandey, D.K., et al. A validated and densitometric HPTLC method for the simultaneous quantification of reserpine and ajmalicine in Rauvolfia serpentina and Rauvolfia tetraphylla. Revista Brasileira de Farmacognosia (2016), http://dx.Available online xxx Keywords:High performance thin layer chromatography Reserpine Ajmalicine R. serpentina and R. tetraphylla a b s t r a c t High performance thin layer chromatographic method (HPTLC) has been developed for the quantification of reserpine and ajmalicine in root part of two different population of Rauvolfia serpentina (L.) Benth. ex Kurz and Rauvolfia tetraphylla L., Apocynaceae, collected from Punjab and Uttarakhand. HPTLC of methanolic extract of root containing indole alkaloids, i.e., reserpine and ajmalicine, was performed on TLC Silicagel 60 F 254 (10 cm × 10 cm) plates with toluene:ethyl acetate:formic acid (7:2:1), as mobile phase. Quantification of the reserpine and ajmalicine was performed in the absorption-reflection mode at 268 nm. The recovery of reserpine and ajmalicine were 99.3 and 98.7% respectively. The calibration curves were linear for both the reserpine and ajmalicine, in the range of 200-1200 ng. HPTLC densitometry has been performed for the estimation of reserpine and ajmalicine in root part of R. serpentina and R. tetraphylla for the first time. The method is simple, rapid and cost effective and can be used for routine analysis of ajmalicine and reserpine in different Rauvolfia species as well as for quality control of herbal drugs containing Rauvolfia species.

Identification of suitable substitute for Sarpagandha (Rauvolfia serpentina (L.) Benth. ex Kurz) by phytochemical and pharmacological evaluation

“…Rauvolfia is well known for its alkaloid content [4] and the same was estimated based on the reaction with bromocresol green (BCG). The highest alkaloid content was showed by R. tetraphylla (0.90%), followed by R. serpentina (0.48 %).…”

“…Leaves are simple, alternate, elliptic lanceolate, 5-10 cm × 3-4 cm, acuminate, peduncle terminal, erect, 4-6 flowered. Rauvolfia verticillata is an erect shrub growing up to 3 m tall and also a prolific source of heterocyclic indole alkaloids [4].…”

High‐performance liquid chromatographic and subcritical fluid chromatographic separation of α‐arylated ß‐carboline, N‐alkylated tetrahydroisoquinolines and their bioisosteres on polysaccharide‐based chiral stationary phases

“…Interest in the research of compounds containing the 1,2,3,4‐tetrahydroisoquinoline ring system has recently come into view, since compounds bearing this skeleton exert major biological activities . Several derivatives are common nucleus in synthetic structures, while other compounds are extracted from natural sources.…”

“…Both enantiomers of 1‐methyl‐6,7‐dimethoxy‐1,2,3,4‐tetrahydroisoquinoline (salsolidine) are naturally occurring compounds . The pharmaceutically valuable 1‐substituted 1,2,3,4‐tetrahydro‐ß‐carboline skeleton is also a common building block of several alkaloids, including the naturally occurring reserpine, which shows antitumor activity as well as antihypertensive and neuroprotective effects .…”

Fluorimetric detection of reserpine in mouse serum through online post-column electrochemical derivatization

“…To date, several works have focused on the analysis of reserpine in tablets [ 4 , 5 ], plants [ 6 – 8 ], plasma [ 9 ] and urine [ 10 ]. Various methods, such as adsorptive stripping voltammetry [ 11 , 12 ], high-performance thin layer chromatography [ 13 ], spectrofluorimetry [ 14 – 16 ], chemiluminescence [ 17 ] and capillary electrophoresis [ 18 , 19 ], have been used for reserpine detection. These methods, however, are unsuitable for the online separation and direct detection of reserpine.…”