Effect of exogenous phytohormones treatment on glycyrrhizic acid accumulation and preliminary exploration of the chemical control network based on glycyrrhizic acid in root of Glycyrrhiza uralensis

Li, Yan-peng; Yu, Chunxia; Qiao, Jing; Zang, Yimei; Yu, Xiaoyan; Ren, Guosheng; Wang, Li; Zhang, Xinyue; Liu, Chunsheng

doi:10.1016/j.bjp.2016.02.009

Cited by 18 publications

(13 citation statements)

References 19 publications

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“…In this study, the positive correlation between GA 3 , ZR, DHZR, MeJA, and active components in the roots suggest that these four phytohormones may also regulate the accumulation of active components. Li et al demonstrated that GA 3 at 40 mg/L applied in June significantly promoted the accumulation of glycyrrhizic acid in G. uralensis root [ 29 ]. However, whether applied GA 3 can be used to increase the content of other active components remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Effect of Abscisic Acid on Accumulation of Five Active Components in Root of Glycyrrhiza uralensis

Qiao

Luo

et al. 2017

Molecules

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Licorice is one of the most generally used herbal medicines in the world; however, wild licorice resources have decreased drastically. Cultivated Glycyrrhiza uralensis Fischer are the main source of licorice at present, but the content of main active components in cultivated G. uralensis are lower than in wild G. uralensis. Therefore, the production of high-quality cultivated G. uralensis is an urgent issue for the research and production fields. In this study, the content of five active components and seven endogenous phytohormones in cultivated G. uralensis (two-year-old) were determined by high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA), respectively. Furthermore, different concentrations (25–200 mg/L) of exogenous abscisic acid (ABA) were sprayed on the leaves of G. uralensis in the fast growing period. Results showed that ABA, zeatin riboside (ZR), and dihydrozeatin riboside (DHZR) had strong correlation with active components. In addition, the content of five active components increased remarkably after ABA treatment. Our results indicate that ABA is significantly related to the accumulation of active components in G. uralensis, and the application of exogenous ABA at the proper concentration is able to promote the accumulation of main components in G. uralensis.

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Section: Discussionmentioning

confidence: 99%

Effect of Abscisic Acid on Accumulation of Five Active Components in Root of Glycyrrhiza uralensis

Qiao

Luo

et al. 2017

Molecules

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“…Glycyrrhiza uralensis Fisch. is a popular medicinal plant generally used in Chinese medicine for its therapeutic actions of nourishing qi, alleviating pain, and tonifying spleen (Li et al 2016). These effects can be attributed to the abundant pharmacologically active substances in its dried roots and rhizomes.…”

Section: Introductionmentioning

confidence: 99%

Growth-promoting bacteria alleviates drought stress of G. uralensis through improving photosynthesis characteristics and water status

Zhang

Xie

Zhang

et al. 2019

Journal of Plant Interactions

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“…However, most cultivated licorice has problems such as low quality, especially the content of GA is quite different from that of the wild licorice, which seriously affects the clinical e cacy of licorice and the development of its application. Therefore, it is of great signi cance to improve the content of GA and produce the high quality cultivated licorice in the research industry and production elds [8][9]. At present, some researchers are devoted to increasing the content of GA in cultivated licorice, such as planting in local areas, improving cultivation techniques [9] [10], improving the cultivation environment [11] [12] and extending planting years [13], etc.…”

Section: Introductionmentioning

confidence: 99%

“…At present, lots of literatures have shown that there is an interconnected and interrelated metabolic network in plant secondary metabolic synthesis pathways [8] [24] . Each secondary metabolite pathway of licorice is not isolated.…”

Section: Introductionmentioning

confidence: 99%

“…There are close connections between the various metabolic pathways. The common substrate is the node to form a network, and the different metabolic pathways within the network inyeract with each other [8]. In previous study, we applied different concentrations of Abscisic acid, Gibberellin, Methyl jasmonate, 6-Benzylaminopurine, Brassinolide and Auxin to licorice.…”

Section: Introductionmentioning

confidence: 99%

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Special Genotypes of ABA Key Functional Genes NCEDs Members in the Glycyrrhizic Acid Synthesis Regulatory Network Promote Glycyrrhizic Acid Synthesis in Licorice

Xuan

Shen

et al. 2021

Preprint

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BackgroundThe glycyrrhizic acid biosynthesis pathway does not exist in isolation, but is connected with the biosynthesis pathways of other secondary metabolites in licorice and finally forms a network. Our previous study found that exogenous spraying of appropriate concentration of abscisic acid (ABA) could increase the content of glycyrrhizic acid (GA) in licorice. However, the mechanism of action remains unknown. We aim to understand the molecular mechanism of ABA promoting the synthesis of GA in licorice and find the molecular marker for the high content of GA germplasm material.MethodsWe analyzed the expression of the key gene of β-AS for GA synthesis after applying ABA, the key functional genes NCED1, NCED3 and NCED4 in the process of ABA synthesis were overexpression, and analyzed the relationship between the SNP polymorphism of the NCED1, NCED3, NCED4 and the content of the GA and ABA in 13 different provenances of licorice with the grey correlation analysis. ResultsThe appropriate concentration of ABA treatment could increase the content of the GA through improving the expression of β-AS. There were significant differences in the content of ABA and GA among the 13 provenances, and the 3 members of the NCEDs family of different provenances had abundant SNP variation sites. Grey correlation and overexpression of NCEDs function both showed that the effect of promoting the synthesis of ABA and GA: NCED1 437 bp G type > NCED3 966 bp G type > NCED4 845 bp A type. All of the above indicated that NCEDs gene variation was the reason for the diversity of GA and ABA content. When selecting high GA germplasm, more priority should be given to NCED1 gene 437 bp G type, NCED3 gene 966 bp G type and NCED4 gene 845 bp A type. ConclusionThis study provides a basis for the selection of excellent GA content germplasm of licorice, and provide some reference for producing a high quality cultivated licorice.

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Effect of exogenous phytohormones treatment on glycyrrhizic acid accumulation and preliminary exploration of the chemical control network based on glycyrrhizic acid in root of Glycyrrhiza uralensis

Cited by 18 publications

References 19 publications

Effect of Abscisic Acid on Accumulation of Five Active Components in Root of Glycyrrhiza uralensis

Effect of Abscisic Acid on Accumulation of Five Active Components in Root of Glycyrrhiza uralensis

Growth-promoting bacteria alleviates drought stress of G. uralensis through improving photosynthesis characteristics and water status

Special Genotypes of ABA Key Functional Genes NCEDs Members in the Glycyrrhizic Acid Synthesis Regulatory Network Promote Glycyrrhizic Acid Synthesis in Licorice

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