Antioxidant, DNA damage protective, neuroprotective, and α-glucosidase inhibitory activities of a flavonoid glycoside from leaves of Garcinia gracilis

Supasuteekul, Chonlakan; Nonthitipong, Wanroong; Tadtong, Sarin; Likhitwitayawuid, Kittisak; Tengamnuay, Parkpoom; Sritularak, Boonchoo

doi:10.1016/j.bjp.2016.01.007

Cited by 18 publications

(21 citation statements)

References 35 publications

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“…Figure 1 shows the electrophoretic pattern of DNA derived from pBR322 plasmid in the absence and presence of EEPS. Band on lane 7 shows pBR322 plasmid DNA in the absence of Fenton reagent, which was mostly in the supercoiled form with high electrophoretic mobility, as similarly observed in other studies [ 33 ]. Exposure of plasmid DNA to •OH radicals was shown to cause DNA strand breakage as indicated by open circular form with low mobility in electrophoreses (band on lane 1).…”

Section: Resultssupporting

confidence: 83%

Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

Simamora

Santoso

Timotius

et al. 2020

International Journal of Food Science

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Premna serratifolia, commonly known as Arogo in Tentena-Sulawesi, is a popular vegetable. As a promising herbal tea and food ingredient, further investigation is required to find the best knowledge for medicinal use of P. serratifolia leaves. This research investigated the antioxidant activity of the ethanol (EEPS) and water (WEPS) extracts of P. serratifolia leaves, based on their scavenging activities on DPPH radicals and their reducing capacities (CuPRAC, total antioxidant/phosphomolybdenum, and ferric thiocyanate reducing power assays). The DNA-protecting effect by EEPS was tested using pBR322 plasmid DNA against •OH radical-induced damage. The inhibition potentials of both extracts against several enzymes related to metabolic diseases (α-glucosidase, α-amylase, xanthine oxidase, and protease) were evaluated. The phytochemical analysis was conducted by an LC-QTOF-MS/MS technique. EEPS proved to be a better antioxidant and had higher phenolic content compared to WEPS. EEPS demonstrated a protective effect on DNA with recovery percentage linearly correlated with EEPS concentrations. Strong inhibition on α-glucosidase and α-amylase was observed for EEPS; however, EEPS and WEPS showed weak inhibitions on xanthine oxidase and protease. LC-QTOF-MS/MS analysis identified seven main components in EEPS, namely scroside E, forsythoside A and forsythoside B, lavandulifolioside, diosmin, nobilin D, campneoside I, and isoacteoside. These components may be responsible for the observed enzymes inhibitions and antioxidant properties. Premna serratifolia leaves can be an appropriate choice for the development of nutraceutical and drug preparations.

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Section: Resultssupporting

confidence: 83%

Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

Simamora

Santoso

Timotius

et al. 2020

International Journal of Food Science

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“…Significant DNA damage protection activity in extracts such as [Barley (Salar et al 2017a); pearl millet (Salar et al 2017b); Mung beans (Xiao et al 2015); Grape seeds (Aybastier et al 2018); Ashtvarga (Giri et al 2017); Amazon moss (Fernandes et al 2018);Sugarcane (Abbas et al 2014); Eulophia nuda Lindl. (Kumar et al 2013); Honey (Habib et al 2014); Carissa carandas leaves (Verma et al 2015); Bael flower (Chandrasekara et al 2018); Teucrium polium and Stachys iberica (Tepe et al 2011); Sphallerocarpus gracilis seeds (Gao et al 2014); Garcinia gracilis leaves (Supasuteekul et al 2016) was reported. Protocol for the preparation of extracts for DNA damage protection activity is summarized in Fig.…”

Section: Use Of Natural Resources For Dna Damage Protection Activitymentioning

confidence: 99%

DNA damage protection: an excellent application of bioactive compounds

Kaur

Purewal

Sandhu

et al. 2019

Bioresour. Bioprocess.

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Discovery of deoxyribonucleic acid (DNA) solved out the mystery of cellular functioning and abnormality in cellular metabolism. Understanding the coding of DNA resulted in enormous medical growth and helps the researchers in finding the genes which trigger major chronic diseases in humans. Further, the structural and sequential analysis brought humans into a new era of medical industry. The advancement in understanding of DNA could be a boon for agricultural sector as it allowed the farmers/breeders to choose better varieties with disease resistant features. In developing nations where the staple foods suffers with micronutrient deficiencies and stress conditions, DNA modifications and repair mechanism could solve out their problems. The role of DNA damaging factors and repair mechanism plays a crucial role in occurrence of certain disorders. Extracts prepared from various natural resources could either stop or slow down the process of DNA damage. This will help to eradicate major disorders related to DNA from human race. Further on the basis of type and dose of natural extracts, it would ease the planning of diets for patients suffering from chronic disorders. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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“…Genus garcinia terdiri dari sekelompok tanaman obat dengan agen terapi yang potensial, hal ini dikarenakan seluruh bagian dari tumbuhan telah banyak digunakan secara global sebagai ethnomedicine yang dibuktikan dengan penelitian (2). Beberapa senyawa tersebut menunjukkan berbagai aktivitas biologis, termasuk inhibitor alpha glukosidase (3), antibakteri (4), antivirus (5), neuroprotektif dan antioksidan (6), sitotoksik (7), antiproliferative (8), antiinflamasi (9). Aktivitas antioksidan adalah salah satu efek farmakologi dari tanaman Garcinia yang sedang dikembangkan.…”

Section: Pendahuluanunclassified

AKTIVITAS ANTIOKSIDAN FRAKSI DARI DAUN Garcinia kydia Roxburgh

Martha¹,

Elya

Hanafi

2020

MFF

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Garcinia kydia Roxburgh merupakan salah satu spesies dari genus garcinia, yang secara khusus belum banyak diketahui aktivitas senyawa bioaktif, namun pada penelitian sebelumnya terbukti berpotensi sebagai agen penghambat α-glukosidase. Pada penelitian ini, akan dilakukan investigasi aktivitas antioksidan terhadap berbagai fraksi yang diperoleh dari daun G. kydia Roxb. Metode penelitian dilakukan dengan menentukan aktivitas antioksidan melalui analisis in-vitro menggunakan microplete reader dengan metode radical scavenging (Metode DPPH) dan ferric reducing antioxidant power (Metode FRAP). Hasil menunjukkan bahwa fraksi yang paling aktif dari ekstrak etil asetat adalah FEA8 (DPPH) dan FEA7 (FRAP), sedangkan fraksi paling aktif dari ekstrak metanol adalah FMT4 (DPPH) dan FMT6 (FRAP). Aktivitas antioksidan tertinggi di antara empat fraksi adalah FEA7 dan FEA8. Aktivitas fraksi FEA7 dan FEA8 ditentukan oleh nilai EC50, sehingga diperoleh masing-masing 9,73 dan 9,26 μg/mL (DPPH); 10,28 dan 13,94 μg/mL (FRAP). Garcinia kydia Roxb. berpotensi sebagai agen antioksidan dengan mekanisme yang berbeda. Sehingga hasil penelitian terkait terdapatnya potensi antioksidan tersebut dapat memberikan alternatif terapi diabetes yang disertai komplikasi stres oksidatif.

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Antioxidant, DNA damage protective, neuroprotective, and α-glucosidase inhibitory activities of a flavonoid glycoside from leaves of Garcinia gracilis

Cited by 18 publications

References 35 publications

Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

DNA damage protection: an excellent application of bioactive compounds

AKTIVITAS ANTIOKSIDAN FRAKSI DARI DAUN Garcinia kydia Roxburgh

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