Laticifer proteins from Plumeria pudica inhibit the inflammatory and nociceptive responses by decreasing the action of inflammatory mediators and pro-inflammatory cytokines

Fernandes, Heliana B.; Machado, Douglas L.; Dias, Juliana R.; Brito, Tarcísio Vieira de; Batista, Jalles Arruda; Silva, Renan O.; Pereira, Anna C. T. C.; Ferreira, Gustavo Portela; Ramos, Márcio V.; Medeiros, Jand Venes R.; Aragão, Karoline S.; Ribeiro, Ronaldo A.; Barbosa, André Luiz R.; Oliveira, Jefferson Soares de

doi:10.1016/j.bjp.2015.05.003

Cited by 20 publications

(15 citation statements)

References 43 publications

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“…Additionally, tissue MDA levels were similar among groups for both acute and subchronic evaluation of LPPp treatment. In fact, we expected LPPp treatment not to interfere in overall values of MPO, GSH and MDA, since LPPp inhibited their alterations when inflammatory process was induced (Fernandes et al, 2015;Santana et al,2018, Oliveira et al,2019Oliveira et al,2021). Additionally, no signs of oxidative damage or inflammatory process were observed in histopathological evaluation of kidneys, livers and spleens of animals treated with LPPp for 10 or 20 days, corroborating the results of biochemical evaluation.…”

Section: Discussionmentioning

confidence: 93%

“…2,432) used for identification of the botanical material collected is deposited at the Delta do Parnaíba Herbarium of Federal University of Piauí, Brazil. The latex collected was processed as previously described (Fernandes et al, 2015). During collection, the latex was placed in distilled water at a dilution ratio of 1:1 (v/v) and the mixture was gently stirred.…”

Section: Plant Materials and Latex Processingmentioning

confidence: 99%

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Acute and Subchronic Toxicity Assessment of Proteins from Plumeria pudica Latex in Mice

Souza¹,

Oliveira²,

Moita³

et al. 2021

RSD

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Latex proteins extracted from Plumeria pudica (LPPp) have anti-inflammatory activity in different experimental models. Considering this relevant result, we evaluated the toxicological aspects of the treatment of mice with LPPp. Acute and subchronic toxicities were determined by daily intraperitoneal administration of 40 mg/kg of LPPp for 10 or 20 days, respectively, and were followed by behavioral, hematological, biochemical and histopathological evaluation. Results showed no significant changes in body weight and organs of animals treated with LPPp. Total and differential blood leukocyte counts of LPPp groups did not differ from controls. There was no significant difference in aspartate aminotransferase, alanine aminotransferase, creatinine and urea measurements for the group treated for 20 days. The level of glutathione in the kidney was significantly higher in animals treated with LPPp in the acute toxicity test, but no differences were observed for the subchronic evaluation. The concentration of malondialdehyde and myeloperoxidase activity in the organs did not differ from controls. Histopathological examination of kidney, spleen and liver tissue of animals treated with LPPp revealed normal structures or reversible alterations. Intraperitoneal LD50 of LPPp was higher than 2,000 mg/kg. The data obtained reveal that LPPp is nontoxic at 40 mg/kg.

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Section: Discussionmentioning

confidence: 93%

Section: Plant Materials and Latex Processingmentioning

confidence: 99%

Acute and Subchronic Toxicity Assessment of Proteins from Plumeria pudica Latex in Mice

Souza¹,

Oliveira²,

Moita³

et al. 2021

RSD

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“…RANKL is produced by osteoblasts and bone marrow stromal cells, and its signal is essential for the induction of osteoclastogenesis, which results in alveolar bone loss (Soedarsono et al, 2006). LPPp was previously documented to reduce the levels of TNFα during inflammatory process (Fernandes et al, 2015;Oliveira et al, 2019), suggesting that the effect of LPPp on bone resorption should occur via modulation of pro-inflammatory cytokines. However, more studies are necessary to clarify this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…The groups were as follows: saline (healthy animals without induction of periodontitis), periodontitis (animals with induced periodontitis and untreated), and LPPp (animals with induced periodontitis and submitted to daily treatment with intraperitoneal administration of LPPp at 40 mg/kg). The dose of 40 mg/kg was chosen because it was the best dose in which LPPp showed anti‐inflammatory and antioxidant activity in other in vivo experimental models (Fernandes et al., 2015; Oliveira et al., 2019; Santana et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

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Latex proteins from Plumeria pudica reduce ligature‐induced periodontitis in rats

et al. 2021

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Background: Previous studies have shown that latex proteins from Plumeria pudica (LPPp) have anti-inflammatory and antioxidant activity. Therefore, the aim of this study was to evaluate the effects in rats of LPPp on ligature-induced periodontitis, an inflammatory disease. Methods:The animals were divided into groups: saline (animals without induction of periodontitis), periodontitis (induced periodontitis and untreated) and LPPp (induced periodontitis and treated with 40 mg/kg). The following parameters were evaluated after 20 consecutive days of treatment: gingival bleeding index (GBI), probing pocket depth (PPD), alveolar bone height (ABH) and gingival myeloperoxidase (MPO) activity. In the hepatic tissue, malondialdehyde (MDA), glutathione (GSH) and histopathological alterations were evaluated. Blood levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured.Results: Significant reduction in GBI, PPD and gingival MPO activity and ABH was seen in animals treated with LPPp compared with periodontitis. Values of GSH, MDA, ALT and histopathological evaluation were preserved in animals treated with LPPp. Conclusions:Treatment with LPPp improved clinical aspects of periodontitis, reduced the blood and hepatic alterations and prevented alveolar bone loss. Data suggest that LPPp have potential for treatment of periodontitis.

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Chemistry, Biological Activities, and Uses of Latex from Selected Species of Apocynaceae

Naidoo¹,

Munsamy²,

Naidoo³

et al. 2021

Reference Series in Phytochemistry

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Laticifer proteins from Plumeria pudica inhibit the inflammatory and nociceptive responses by decreasing the action of inflammatory mediators and pro-inflammatory cytokines

Abstract: Please cite this article in press as: Fernandes, H.B., et al. Laticifer proteins from Plumeria pudica inhibit the inflammatory and nociceptive responses by decreasing the action of inflammatory mediators and pro-inflammatory cytokines. Revista Brasileira de Farmacognosia (2015), http://dx.

Cited by 20 publications

References 43 publications

Acute and Subchronic Toxicity Assessment of Proteins from Plumeria pudica Latex in Mice

Acute and Subchronic Toxicity Assessment of Proteins from Plumeria pudica Latex in Mice

Latex proteins from Plumeria pudica reduce ligature‐induced periodontitis in rats

Chemistry, Biological Activities, and Uses of Latex from Selected Species of Apocynaceae

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