Fucoidan induces G1 arrest of the cell cycle in EJ human bladder cancer cells through down-regulation of pRB phosphorylation

Park, Hye Young; Choi, Il‐Whan; Kim, Gi‐Young; Kim, Byung Woo; Kim, Wun-Jae; Choi, Yung Hyun

doi:10.1016/j.bjp.2015.03.011

Cited by 20 publications

(18 citation statements)

References 37 publications

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“…A wide variety of previous studies reported cell cycle arrest in G1 or G2/M phase after fucoidan treatment [22,36,46]. A previous study showed that fucoidan from F. vesiculosus caused G0/G1 arrest of HT29 colon cancer cells after 24 h of treatment and it was dose-dependent (0, 50 and 100 µg/mL).…”

Section: Fucoidan Causes G1 Phase Cell Cycle Arrest In Mg63 Cellsmentioning

confidence: 96%

“…Recently, it has been highlighted that cell proliferation, apoptosis, adhesion, invasion and metastasis represent potential biological targets for treating osteosarcoma [35], and that one of the most potent natural drugs for this application may be fucoidan. Fucoidanhas been shown to inhibit cancer cells by inducing apoptosis via several mechanisms [21,22,24,29,33,[36][37][38][39][40][41], such as inhibition of cell proliferation and invasion by targeting cell cycle proteins and the PI3K-Akt-mTOR pathway [42], inhibition of Vascular Endothelial Growth Factor and Matrix Metalloproteinases [30], enhancing ubiquitin-dependent TGFβ receptor degradation [23,43] and regulating the miR-29c/ADAM12 and miR-17-5p/PTEN axes [44].This study simultaneously compares the anti-cancer activities of crude fucoidans from two brown seaweed species F. vesiculosus and S. filipendula, and the effects of low-, medium-and high-MW (LMW, MMW and HMW) fractions of fucoidan derived from S. filipendula against MG63 osteosarcoma cells. Using size-fractionated fucoidan, we also demonstrate that as the molecular weight of fucoidan increases, the anti-cancer activity of fucoidan also increases.…”

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confidence: 99%

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Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

et al. 2020

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Fucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anti-cancer activities of crude fucoidans from Fucus vesiculosus and Sargassum filipendula, and effects of low (LMW, 10–50 kDa), medium (MMW, 50–100 kDa) and high (HMW, >100 kDa) molecular weight fractions of S. filipendula fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in F. vesiculosus crude fucoidan compared to S. filipendula crude fucoidan. MMW had the highest levels of sugars, acids and sulphation among molecular weight fractions. There was a dose-dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but F. vesiculosus fucoidan and HMW had the strongest effects. G1-phase arrest was induced by F. vesiculosus fucoidan, MMW and HMW, however F. vesiculosus fucoidan treatment also caused accumulation in the sub-G1-phase. Mitochondrial damage occurred for all fucoidan-types, however F. vesiculosus fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress-induced apoptosis-like cell death for F. vesiculosus fucoidan and features of stress-induced necrosis-like cell death for S. filipendula fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.

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Section: Fucoidan Causes G1 Phase Cell Cycle Arrest In Mg63 Cellsmentioning

confidence: 96%

mentioning

confidence: 99%

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

et al. 2020

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“…A and B: One Way ANOVA, using Tukey's multiple comparisons test, indicated that the differences in the mean values among the treatment groups are greater than would be expected by chance, i.e., there is a statistically significant difference (p<0.001), except for treatments indicated in the text. [7,8,10,17,20,21,61,62,426,459,471,472,482,483,494,510,515,[517][518][519][520][521].…”

Section: And Mcf-12a Cellsmentioning

confidence: 99%

“…The significant elevation in the extent of cell death in MCF-7 cells is associated with apoptotic hallmarks. In this regard, it has recently been demonstrated that FUC induced G1 arrest of the cell cycle in EJ human bladder cancer cells via downregulation of pRB phosphorylation [517]. An earlier http://etd.uwc.ac.za/ report also asserted that FUC stimulated G1 arrest of the cell cycle in cultured human nonsmall-cell bronchopulmonary carcinoma cells [422].…”

Section: Cell Cyclementioning

confidence: 99%

Effects of Fucoidan and Chemotherapeutic Agent Combinations on Malignant and Non-malignant Breast Cell Lines

Abudabbus

Badmus

Shalaweh

et al. 2017

CPB

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Fucoidan is an effective antitumor agent, either alone or in combination with cisplatin, doxorubicin and taxol in MCF-7 breast cancer cells. Drug combinations that discriminate between cancerous and non-cancerous cells afford a plausible and viable strategy of attaining therapeutic efficacy and avoiding possible toxicity and side effects. These findings suggest that fucoidan is a promising candidate for cancer combination therapies.

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“…The 5year survival rate for osteosarcoma is 60%-70%. Recently, it has been highlighted that cell proliferation, apoptosis, adhesion, invasion, and metastasis represent potential biological targets for treating osteosarcoma [35] and one of the most potent natural drugs for this application may be fucoidan as it has been shown to inhibit cancer cells by inducing apoptosis via several mechanisms [21,22,41,24,29,33,[36][37][38][39][40] such as inhibition of cell proliferation and invasion by targeting cell cycle proteins and PI3K-Akt-mTOR pathway [42], inhibition of Vascular Endothelial Growth Factor and Matrix Metalloproteinases [30], enhancing ubiquitin-dependent TGFβ receptor degradation [23,43] and regulating the miR-29c/ADAM12 and miR-17-5p/PTEN axes [44].…”

mentioning

confidence: 99%

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

Gupta¹,

Silva²,

Radziun³

et al. 2019

Preprint

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Fucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anticancer activities of crude fucoidans from Fucus vesiculosus and Sargassum filipendula, and effects of low (LMW, 10-50kDa), medium (MMW, 50-100kDa) and high (HMW, >100kDa) molecular weight fractions of S. filipendula fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in F. vesiculosus crude fucoidan compared to S. filipendula crude fucoidan. MMW had highest the levels of sugars, acids and sulphation among molecular weight fractions. There was a dose dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but F. vesiculosus fucoidan and HMW had the strongest effects. G1-phase arrest was induced by F. vesiculosus fucoidan, MMW and HMW, however F. vesiculosus fucoidan treatment also caused accumulation in sub G1-phase. Mitochondrial damage occurred for all fucoidan-types, however F. vesiculosus fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress induced apoptosis-like cell death for F. vesiculosus fucoidan but features of stress-induced necrosis-like cell death for S. filipendula fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.

show abstract

Fucoidan induces G1 arrest of the cell cycle in EJ human bladder cancer cells through down-regulation of pRB phosphorylation

Cited by 20 publications

References 37 publications

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

Effects of Fucoidan and Chemotherapeutic Agent Combinations on Malignant and Non-malignant Breast Cell Lines

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

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