Essential oil from Ageratum fastigiatum reduces expression of the pro-inflammatory cytokine tumor necrosis factor-alpha in peripheral blood leukocytes subjected to in vitro stimulation with phorbol myristate acetate

Avelar-Freitas, Bethânia Alves; Almeida, Valéria Gomes de; Santos, Michaelle Geralda dos; Barroso, Poliana Ribeiro; Grael, Cristiane Fernanda Fuzer; Gregório, Luiz Elídio; Rocha‐Vieira, Etel; Brito-Melo, Gustavo Eustáquio Alvim

doi:10.1016/j.bjp.2015.03.002

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…Specially, at the 10% v/v concentration, the inhibitory effect on IFN-γ production was nearly 5 times greater than the cyclosporine effect for the assessed cell populations. Avelar-Freitas et al [ 24 ] observed that concentrations up to 1% v/v of DMSO did not change IFN-γ and TNF-α production when they were evaluated in the cytoplasm of human lymphocytes and neutrophils stimulated with PMA for 4 h. When evaluating DMSO anti-inflammatory activity over whole blood samples using ELISA assay, during 7 h incubation, Elisia et al (2016) [ 25 ] verified that DMSO reduced the secretion of 13 cytokines important in inflammatory response. It was shown that 0.5% DMSO concentration is able of inhibit IFN-γ secretion, while 2% inhibits TNF-α secretion.…”

Section: Discussionmentioning

confidence: 99%

Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and TNF-α, IFN-γ, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes

et al. 2017

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Dimethylsulfoxide (DMSO) is an amphipathic molecule composed of a polar domain characterized by the sulfinyl and two nonpolar methyl groups, for this reason it is able to solubilize polar and nonpolar substances and transpose hydrophobic barriers. DMSO is widely used to solubilize drugs of therapeutic applications and studies indicated that 10% v/v concentration did not modify culture viability when used to treat human peripheral blood mononuclear cells (PBMC). However, some DMSO concentrations could influence lymphocyte activation and present anti-inflammatory effects. Therefore, the objective of this study was to evaluate the effect of DMSO on lymphocyte activation parameters. Cell viability analysis, proliferation, and cytokine production were performed on PBMC from six healthy subjects by flow cytometry. The results indicated that 2.5% v/v DMSO concentrations did not modify lymphocytes viability. DMSO at 1% and 2% v/v concentrations reduced the relative proliferation index of lymphocytes and at 5% and 10% v/v concentrations reduced the percentage of total lymphocytes, cluster of differentiation 4+ (CD4+) T lymphocytes and CD8+ T lymphocytes interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) producers. Thus, it was concluded that DMSO has an in vitro anti-inflammatory effect by reducing lymphocyte activation demonstrated with proliferation reduction and the decrease of cytokine production.

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Section: Discussionmentioning

confidence: 99%

Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and TNF-α, IFN-γ, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes

et al. 2017

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“…The in vitro methods of anti-inflammatory potential evaluation include leukocyte viability tests and effects on the release of inflammatory or pro-inflammatory mediators such as interleukins, cytokines, and TNF-α, evaluation of inhibition of NO, COX and PGE inhibition assay, and NF-κB assay [23,30,33,45].…”

Section: Comparison Between Methods Used For Pharmacological Evaluatimentioning

confidence: 99%

“…Evaluation of inhibition of NO production in lipopolysaccharide-induced macrophages [30] Inhibition of COX and PGE [33] Leukocyte modulation and viability and production of cytokines and NO [23] Inhibition of NF-κB [45] In vivo…”

Section: In Vitromentioning

confidence: 99%

Anti-Inflammatory Activity of the Compositae Family and Its Therapeutic Potential

Lopes

Oliveira²,

Viçosa³

et al. 2020

Planta Med

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Compositae is the largest family of flowering plants, with more than 1600 genera and 22 000 species. It has many economic uses in foods, cosmetics, and pharmaceutics. The literature reports its numerous medicinal benefits and recognized anti-inflammatory activity. Thus, this study evaluated the technological trends of anti-inflammatory activity of Compositae, based on the survey of scientific databases, articles, and patents, as well as the website of the Brazilian National Health Regulatory Agency (ANVISA), which is responsible for registering and controlling of healthcare and cosmetic products in the Brazil. The survey was conducted between 2008 and 2018, in the databases Science Direct, Lilacs, PubMed, and Web of Science (main collection), as well as the SciELO Citation Index. The patent survey was carried out on the basis of the Derwent Innovations Index, an important source for worldwide patent consultation, which covers 20 y of registered patents. Despite the numerous studies involving species of the Compositae family in different models of anti-inflammatory activity, there are few records of patents or products on the market from these species for that purpose. Some species have a traditional use and are present even in the Phytotherapic Summary of the Brazilian Pharmacopeia. This review confirms the therapeutic potential of Compositae for the development of anti-inflammatory drugs and reinforces the need to develop competencies and reduce technological bottlenecks to promote research and innovation in biodiversity products.

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“…Estudos acerca do potencial biológico de óleos essenciais e extratos polares de Ageratum fastigiatum revelaram atividades antimicrobianas, analgésicas e anti-inflamatórias. Apesar do seu uso popular, pouco se sabe acerca do seu potencial sobre os parâmetros envolvidos em uma resposta inflamatória (AVELAR-FREITAS et al, 2015). Pode ser localizada em áreas antropogênicamente perturbadas e em áreas naturais do planalto da cidade de Diamantina.…”

Section: Ageratum Fastigiatumunclassified

“…Dentre as espécies, encontram-se plantas das famílias Asteraceae, Euphorbiacea, Fabaceae (AVELAR-FREITAS et al, 2015;AVELAR et al, 2011). Nota-se a necessidade de reunir informações existentes sobre essas plantas, a fim de se avançar nos estudos de bioprospecção, garantindo uso seguro e racional de tais plantas.…”

Section: Introductionunclassified

Plantas Medicinais Utilizadas na Região de Diamantina (MG), Cerrado e Campo Rupestre da Serra do Espinhaço (MG)

Campos

Avelar-Freitas

2018

Ens Cien C Biol Agra Saúde

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O uso de plantas medicinais é praticado na medicina popular, sobretudo, nos países em desenvolvimento. As plantas medicinais são elementos que constituem parte da biodiversidade e são largamente utilizadas desde os primórdios da civilização por vários povos e de diversas maneiras. Estas plantas têm se mostrado de importante relevância como medicamentos caseiros, visto que em suas composições possuem constituintes químicos eficazes para o tratamento de certas doenças. Neste estudo, o objetivo foi realizar uma revisão bibliográfica acerca das plantas medicinais utilizadas no município de Diamantina (MG) e seus entornos, a fim de compilar as informações obtidas para auxiliar nos avanços dos estudos. Utilizando os descritores Plantas medicinais, Diamantina e fitoterápicos foram encontrados 10 artigos, sendo as plantas mais citadas as espécies: Ageratum fastigiatum e Euphorbia tirucalli, as quais apresentaram as atividades sobre células do sistema imune em estudos in vitro. Novas pesquisas e estudos ainda são necessários para comprovação de propriedades farmacológicas e padronização do uso, portanto, ainda há poucas informações sobre a segurança e a confiabilidade das plantas medicinais e novos estudos devem ser apurados acerca do assunto.Palavras-chave: Plantas Medicinais. Vale do Jequitinhonha. Diamantina.AbstractThe use of medicinal plants is practiced by many populations, especially in developing countries. Medicinal plants are elements that constitute part of biodiversity and have been widely used since the dawn of civilization by various peoples and in various ways. They have been shown to be of significant relevance as home remedies, since in their compositions they have effectice chemical constituents for the treatment of certain diseases. In this work the objective was to carry out a bibliographical review about the medicinal plants used in the city of Diamantina (MG) and its surroundings, in order to compile the obtained information to support in the studies advances. Using the descriptors Medicinal plants, Diamantina and phytorerapics, 10 articles were found, the most cited being the species Ageratum fastigiatum and Euphorbia tirucalli, which presented the activities on the cells of the immune system in vitro studies. New research and studies are still necessary to prove pharmacological properties and standardization of the use, therefore, there is still little information about the safety and reliability of medicinal plants and new studies should be determined on the subject.Keywords: Medicinal Plants. Jequitinhonha Valley. Diamantina.

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Essential oil from Ageratum fastigiatum reduces expression of the pro-inflammatory cytokine tumor necrosis factor-alpha in peripheral blood leukocytes subjected to in vitro stimulation with phorbol myristate acetate

Cited by 8 publications

References 21 publications

Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and TNF-α, IFN-γ, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes

Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and TNF-α, IFN-γ, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes

Anti-Inflammatory Activity of the Compositae Family and Its Therapeutic Potential

Plantas Medicinais Utilizadas na Região de Diamantina (MG), Cerrado e Campo Rupestre da Serra do Espinhaço (MG)

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