Halistanol sulfate A and rodriguesines A and B are antimicrobial and antibiofilm agents against the cariogenic bacterium Streptococcus mutans

Lima, Bruna de Araújo; Lira, Simone Possedente de; Kossuga, M. H.; Gonçalves, Reginaldo Bruno; Berlinck, Roberto G. S.; Kamiya, Regianne Umeko

doi:10.1016/j.bjp.2014.11.002

Cited by 10 publications

(8 citation statements)

References 52 publications

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“…The minimal inhibitory concentration (MIC) test or broth microdilution assay was carried out using microplates (96 wells), following the procedure according to the Clinical and Laboratory Standards Institute [ 32 ] and Lima et al [ 33 ] for bacteria and EUCAST [ 34 ] for yeast, with some modifications. The McFarland Scale 0.5 determined the cell concentration.…”

Section: Methodsmentioning

confidence: 99%

Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

Porto

Almeida

Costa

et al. 2018

BMC Complement Altern Med

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BackgroundPropolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface.MethodsAn ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b* chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05).ResultsThe UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3.ConclusionsThe EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface.

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Section: Methodsmentioning

confidence: 99%

Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

Porto

Almeida

Costa

et al. 2018

BMC Complement Altern Med

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“…Minimal inhibitory response [8] MIC was determined by microtiter broth dilution method. The suspension of bacteria in as much as 100 μl of 10 −5 concentration was added to 96-well plates according to the group.…”

Section: Determining Minimal Inhibitory Response and Minimum Bactericmentioning

confidence: 99%

Potential of Javanese Turmeric Ethanol Extract in Inhibiting Streptococcus Sanguinis and Porphyromonas Gingivalis Biofilm Formation

Hutauruk

Suniarti²,

Djohan

2019

Int J App Pharm

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Objective: The purpose of the current study was to evaluate the potential of Javanese turmeric ethanol extract in the inhibition of the formation ofStreptococcus sanguinis and Porphyromonas gingivalis biofilms, individually and in combination.Methods: The concentration of P. gingivalis and S. sanguinis bacteria ranged from 0.5% to 25%. Inhibition assay for biofilm formation was conductedon a 96-well plate using brain heart infusion (BHI) agar enriched with 0.2% sucrose at 37oC for 18 h. After staining with 0.5% crystal violet, the opticaldensity was measured at 490 nm.Results: Javanese turmeric can potentially inhibit the biofilm formation of S. sanguinis (IC50 0.5%, IC90 15%) and P. gingivalis (IC50 15%) on single anddual species (IC50 0.5%, IC90 15%).Conclusion: Javanese turmeric has the potential to inhibit the formation of S. sanguinis and P. gingivalis biofilms.

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“…Compound 95 inhibited biofilm formation in two S. mutans strains at low MIC, but did not inhibit initial colonization by S. sanguinis . Such activity is highly desirable in preventative treatments that inhibits pathogenic bacteria via the disruption of biofilm formation without affecting the healthy normal microflora [ 64 ].…”

Section: Lipidsmentioning

confidence: 99%

“…were found to possess broad antimicrobial activities inhibiting both oral streptococci and pathogenic bacteria. These diketopiperazines are reported to modulate the LuxR-mediated quorum-sensing systems of Gram-negative and Gram-positive bacteria and are considered to influence cell-cell bacterial signaling, offering alternative ways to control biofilms by interfering with microbial communication [ 64 ]. Diketopiperazines, cyclo-( l -valyl- d -proline) ( 97 ) cyclo-( l -phenylalanyl- d -proline ( 98 ), were isolated from a Rheinheimera japonica strain KMM 9513 collected from shores off of the Sea of Japan.…”

Section: Peptidesmentioning

confidence: 99%

Current Status and Future Prospects of Marine Natural Products (MNPs) as Antimicrobials

et al. 2017

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The marine environment is a rich source of chemically diverse, biologically active natural products, and serves as an invaluable resource in the ongoing search for novel antimicrobial compounds. Recent advances in extraction and isolation techniques, and in state-of-the-art technologies involved in organic synthesis and chemical structure elucidation, have accelerated the numbers of antimicrobial molecules originating from the ocean moving into clinical trials. The chemical diversity associated with these marine-derived molecules is immense, varying from simple linear peptides and fatty acids to complex alkaloids, terpenes and polyketides, etc. Such an array of structurally distinct molecules performs functionally diverse biological activities against many pathogenic bacteria and fungi, making marine-derived natural products valuable commodities, particularly in the current age of antimicrobial resistance. In this review, we have highlighted several marine-derived natural products (and their synthetic derivatives), which have gained recognition as effective antimicrobial agents over the past five years (2012–2017). These natural products have been categorized based on their chemical structures and the structure-activity mediated relationships of some of these bioactive molecules have been discussed. Finally, we have provided an insight into how genome mining efforts are likely to expedite the discovery of novel antimicrobial compounds.

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Halistanol sulfate A and rodriguesines A and B are antimicrobial and antibiofilm agents against the cariogenic bacterium Streptococcus mutans

Cited by 10 publications

References 52 publications

Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

Potential of Javanese Turmeric Ethanol Extract in Inhibiting Streptococcus Sanguinis and Porphyromonas Gingivalis Biofilm Formation

Current Status and Future Prospects of Marine Natural Products (MNPs) as Antimicrobials

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