Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin

Abstract

CopaibaLarvicidal Nanoemulsion Oleoresin A B S T R A C T Copaiba (Copaifera duckei Dwyer, Fabaceae) oleoresin is an important Amazonian raw material. Despite its insecticidal potential, poor water solubility remains a challenge for the development of effective and viable products. Nanotechnology has emerged as a promising area to solve this problem, especially oil-in-water nanoemulsions. On this context, the aim of the present study was to develop oil-in-water nanoemulsions using copaiba oleoresin dispersed through a high internal phase; and evaluate its potential insecticidal action against Aedes aegypti larvae. Overall, 31 formulations were prepared, ranging from 11.5 ± 0.2 to 257.3 ± 4.1 nm after one day of manipulation. Some of them reached small mean droplet sizes (< 200 nm) and allowed achievement of a nanoemulsion region. The formulation consisted of 5% (w/w) of copaiba oil, 5% (w/w) of surfactant and 90% (w/w) of water, which presented mean droplet size of 145.2 ±0.9 nm and polidispersity of 0.378 ± 0.009 after one day of manipulation, and these were evaluated for larvicidal potential. According to mortality level (250 ppm -93.3 after 48 h), this nanoemulsion was classified as a promising insecticidal agent against Aedes aegypti larvae. The present study allowed the development of low-cost ecofriendly green natural-based nanoformulations with potential larvicidal activity, using a nanobiotechnology approach.

Effects of a nanoemulsion with Copaifera officinalis oleoresin against monogenean parasites of Colossoma macropomum: A Neotropical Serrasalmidae

“…oleoresin (Dias et al., ). The oleoresin from Copaifera duckei was heated prior to emulsification, as a means for generating oil‐in‐water nanoemulsions (Rodrigues et al., ). However, it is worth mentioning that some loss of volatiles may occur on this step.…”

“…The mean droplet size of nanoemulsions is often within the range of 20-200 nm, although this criterion may vary according to different authors (Bajerski et al, 2016;Solans, Izquierdo, Nolla, & Azemar, 2005; Sol e, Solans, Maestro, Gonz alez, & Guti errez, 2012;Thomas et al, 2013;Valentim et al, 2018). The main advantages of nanoemulsions are system stability, potential improvement of bioavailability, enhanced water solubility (oil-in-water nanoemulsion), and other properties are due precisely to the nanosized particles (Ostertag, Weiss, & McClements, 2012;Rodrigues et al, 2014). The intrinsic low solubility or immiscibility of internal phase components makes this type of novel delivery system very promising for controlling fish diseases (Rodrigues et al, 2014;Thomas et al, 2013).…”

“…The main advantages of nanoemulsions are system stability, potential improvement of bioavailability, enhanced water solubility (oil-in-water nanoemulsion), and other properties are due precisely to the nanosized particles (Ostertag, Weiss, & McClements, 2012;Rodrigues et al, 2014). The intrinsic low solubility or immiscibility of internal phase components makes this type of novel delivery system very promising for controlling fish diseases (Rodrigues et al, 2014;Thomas et al, 2013). Moreover, nanosize potentially increases the bioactivity of the compounds used (Irache et al, 2011), such that this is very promising for effective treatment, even at lower concentrations than usual for antiparasitic products.…”

Pequiá-Based Nanoemulsion Highlights an Important Amazon Fruit (caryocar villosum (aubl.) pers.)

“…Considering that small droplets, including in the range of nanoemulsions, are intrinsically associated to enhancement of physical stability, it is worth mentioning that rHLB determination is a useful tool to generate nanoemulsions. On this context, several studies with this approach obtained plant-based nanoemulsions using natural oils [18,[20][21][22][23]32,33].…”

“…They are very promising to enhance water solubility of vegetal oils and improve chemical stability and bioavailability of bioactive substances. On this context, several studies aiming to obtain nanoemulsions were carried out with important oils from natural origin [18][19][20][21][22][23]. However, despite great biological potential of Caryocar villosum fruits, to our knowledge, no study was carried out aiming to obtain nanoemulsions using its oil.…”