Diagnostic utility of salivary pepsin in laryngopharyngeal reflux: a systematic review and meta-analysis

Jing, Wuyang; Luo, Wenlong; Lou, Lan

doi:10.1016/j.bjorl.2022.10.050

Cited by 7 publications

(2 citation statements)

References 39 publications

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“…Frontiers in Pharmacology frontiersin.org existing studies use different detection methods, reagent manufacturers and production batch numbers, resulting in different detection results (Jing et al, 2023). Furthermore, saliva dilution and esophageal clearance may lead to low concentrations of pepsin that cannot be detected.…”

Section: Discussionmentioning

confidence: 99%

Tonghua Liyan granules in the treatment of Laryngopharyngeal reflux disease with stagnation of phlegm and qi syndrome: a randomized, double-blind, placebo-controlled study

Li,

Zheng,

Wang

et al. 2024

Front. Pharmacol.

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Background: Laryngopharyngeal reflux disease (LPRD) is an extraesophageal syndromic manifestation of gastroesophageal reflux disease (GERD). Despite the increasing incidence of and concern about LPRD, treatment with proton pump inhibitors (PPIs) is unsatisfactory. Here, LPRD was treated with Tonghua Liyan (THLY) granules in combination with PPIs to evaluate treatment efficacy and possible adverse reactions.Methods: Seventy-six LPRD patients with stagnation of phlegm and qi syndrome (SPQS) were randomly divided into an experimental group and a control group. The experimental group received THLY granules combined with rabeprazole capsules. The control group received THLY granule placebo combined with rabeprazole capsules. A parallel, randomized, double-blind, placebo-controlled clinical trial was conducted with these two groups. The treatment cycle was 8 weeks. The reflux symptom index (RSI), clinical symptom score, salivary pepsin content, reflux finding score (RFS) and gastroesophageal reflux disease questionnaire (GerdQ) were used to evaluate clinical efficacy. The final efficacy rate was evaluated according to the RSI and clinical symptom score.Results: Compared with those at baseline, all the indicators in the experimental group and control group significantly improved (p < 0.01). In terms of the RSI, clinical symptom score, and RFS, the experimental group had a higher degree of improvement (p < 0.05), and the overall efficacy rate was higher (p < 0.05). In terms of the salivary pepsin concentration and GerdQ, there was no significant difference between the test group and the control group (p > 0.05). Both groups of safety indicators showed no abnormalities and did not cause any allergic reactions in the body.Conclusion: Compared with PPIs alone, THLY granules combined with PPIs are more effective in the treatment of LPRD patients with SPQS in terms of symptoms and signs. This combination treatment, because of its higher clinical efficacy and lack of obvious adverse reactions, is worthy of clinical promotion and further in-depth study.Clinical Trial Registration:www.chictr.org.cn, identifier ChiCTR2100046614

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Section: Discussionmentioning

confidence: 99%

Tonghua Liyan granules in the treatment of Laryngopharyngeal reflux disease with stagnation of phlegm and qi syndrome: a randomized, double-blind, placebo-controlled study

Li,

Zheng,

Wang

et al. 2024

Front. Pharmacol.

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“… 4 The association between LPR and laryngeal carcinoma seems to be emerging. 5 , 6 Lifestyle modifications, such as weight loss, stopping tobacco/alcohol use, and refraining from lying down within three hours after dinner, can reduce LPRD. 7 Medications, including histamine-2 receptor antagonists and proton pump inhibitors, can help suppress acid production.…”

Section: Introductionmentioning

confidence: 99%

Analysis on the pharyngeal microbiota in patients with laryngopharyngeal reflux disease

Zhang,

Fan

2023

Brazilian Journal of Otorhinolaryngology

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Acidic Pepsin Affects Laryngeal Carcinoma Cell Growth and Invasion Through Glycolysis

Yu,

Li,

Bao

et al. 2024

Otolaryngol.--head neck surg.

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ObjectiveThe pathogenic mechanism underlying the effects of acidic pepsin in laryngeal cancer remains unclear. This study investigated whether acidic pepsin influences Glut‐1 expression and glycolytic activity in laryngeal carcinoma cells and whether it plays a role in the growth and migration of these cells through glycolysis.Study DesignIn vitro study.SettingA university‐affiliated hospital.MethodsLaryngeal carcinoma TU 212 and TU 686 cells were treated with acidic pepsin and 2‐deoxy‐d‐glucose (2‐DG), then transfected with Glut‐1 small interfering RNA (siRNA). Glucose uptake was detected by a radioimmunoassay counter, lactate secretion was detected by a lactic acid kit, and Glut‐1 expression was detected by western blotting. Cell viability, migration and invasion, and clonal formation were assessed using the Cell Counting Kit‐8, Transwell chamber, and clonal formation assays, respectively.ResultsAcidic pepsin significantly increased Glut‐1 expression in laryngeal carcinoma cells compared with the control group (P < .01). It also significantly enhanced 18F‐fluorodeoxyglucose (Cin/Cout) uptake, lactate secretion, cell viability, migration, invasion, and clonal formation in laryngeal carcinoma cells compared with the control group (P < .01). The glycolytic inhibitor 2‐DG and Glut‐1 siRNA significantly reversed the effects of acidic pepsin on laryngeal carcinoma cells (P < .01).ConclusionAcidic pepsin enhances the growth and migration of laryngeal carcinoma cells by upregulating Glut‐1, thus promoting glycolysis.

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Diagnostic utility of salivary pepsin in laryngopharyngeal reflux: a systematic review and meta-analysis

Cited by 7 publications

References 39 publications

Tonghua Liyan granules in the treatment of Laryngopharyngeal reflux disease with stagnation of phlegm and qi syndrome: a randomized, double-blind, placebo-controlled study

Tonghua Liyan granules in the treatment of Laryngopharyngeal reflux disease with stagnation of phlegm and qi syndrome: a randomized, double-blind, placebo-controlled study

Analysis on the pharyngeal microbiota in patients with laryngopharyngeal reflux disease

Acidic Pepsin Affects Laryngeal Carcinoma Cell Growth and Invasion Through Glycolysis

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