Sheep as a large animal model for cochlear implantation

Trinh, Thuy-Trân; Cohen, Clara; Boullaud, L.; Cottier, Jean-Philippe; Bakhos, D.

doi:10.1016/j.bjorl.2021.02.014

Cited by 12 publications

(6 citation statements)

References 23 publications

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“…The fenestra cochleae was easily observed in the large hypotympanic recess suggesting that surgical training for electrode array insertion or extraction of perilymph samples could be performed in rabbit. This result was in accordance with Trinh et al (2022) in sheep. Our CT images could not define all turns of the cochlea and its internal anatomy; in contrast to Ekdale (2013) in placental mammals and Joshi et al (2012) in humans, this may be due to a low quality CT scanner that used in our study.…”

Section: Discussionsupporting

confidence: 93%

Anatomy of the rabbit inner ear using computed tomography and magnetic resonance imaging

El-Hameed

El-Shafey

Metwally

et al. 2023

Anat Histol Embryol

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Anatomically, the inner ear is a highly complex organ of intricate design, composed of a bony labyrinth that encases the same-shaped membranous labyrinth. It is difficult to study the three-dimensional anatomy of the inner ear because the relevant structures are very small and embedded within the petrous temporal bone, one of the densest bones in the body. The current study aimed to provide a detailed anatomic reference for the normal anatomy of the rabbit's inner ear. As a study model, ten healthy adults New Zealand White rabbit heads were used. Six heads were used for macroscopic evaluation of the bony and membranous labyrinths. The remaining four heads were evaluated radiographically, where 3D images were generated of the bony and membranous labyrinths using data sets from computed tomography (CT) and magnetic resonance imaging (MRI), respectively. The anatomical structures were identified and labelled according to NominaAnatomicaVeterinaria (NAV). Our study revealed that CT and MRI are the optimal cross-sectional imaging modalities for investigating such tiny and often inaccessible inner ear structures. As high-quality scanners are not readily available to veterinarians, the CT and MRI images generated by this research were of lower quality; therefore, high-quality dissections were used to identify/support structures seen in these images. In conclusion, this study provides one of the first investigations that uses multislice CT scans and MRI to study the rabbit's inner ear and its correlation with the corresponding anatomical images. Both anatomical, CT and MRI images will serve as a reference for interpreting pathologies relative to the rabbit's inner ear.

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Section: Discussionsupporting

confidence: 93%

Anatomy of the rabbit inner ear using computed tomography and magnetic resonance imaging

El-Hameed

El-Shafey

Metwally

et al. 2023

Anat Histol Embryol

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“…Yet, rodents show little anatomical, biological, or metabolic similarity to humans with respect to the auditory system [ 12 ]. Anatomically, the sheep cochlea has two and a half turns, similar to humans, and has been investigated in a computed tomography study [ 13 ]. Unlike the guinea pig, the anatomy of the round window membrane of the sheep shows multiple similarities with humans [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…In rodents and small animals, it is difficult to collect cochlear fluids such as perilymph given the anatomy of the inner ear. In large animals, the perilymph volume is larger and easier to sample through the round window [ 13 , 18 , 19 ]. For these reasons, sheep may be a better animal model and was therefore used in the present study.…”

Section: Discussionmentioning

confidence: 99%

Immediate-Early Modifications to the Metabolomic Profile of the Perilymph Following an Acoustic Trauma in a Sheep Model

Boullaud

Blasco

Caillaud

et al. 2022

JCM

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The pathophysiological mechanisms of noise-induced hearing loss remain unknown. Identifying biomarkers of noise-induced hearing loss may increase the understanding of pathophysiological mechanisms of deafness, allow for a more precise diagnosis, and inform personalized treatment. Emerging techniques such as metabolomics can help to identify these biomarkers. The objective of the present study was to investigate immediate-early changes in the perilymph metabolome following acoustic trauma. Metabolomic analysis was performed using liquid chromatography coupled to mass spectrophotometry to analyze metabolic changes in perilymph associated with noise-induced hearing loss. Sheep (n = 6) were exposed to a noise designed to induce substantial hearing loss. Perilymph was collected before and after acoustic trauma. Data were analyzed using univariate analysis and a supervised multivariate analysis based on partial least squares discriminant analysis. A metabolomic analysis showed an abundance of 213 metabolites. Four metabolites were significantly changed following acoustic trauma (Urocanate (p = 0.004, FC = 0.48), S-(5’-Adenosyl)-L-Homocysteine (p = 0.06, FC = 2.32), Trigonelline (p = 0.06, FC = 0.46) and N-Acetyl-L-Leucine (p = 0.09, FC = 2.02)). The approach allowed for the identification of new metabolites and metabolic pathways involved with acoustic trauma that were associated with auditory impairment (nerve damage, mechanical destruction, and oxidative stress). The results suggest that metabolomics provides a powerful approach to characterize inner ear metabolites which may lead to identification of new therapies and therapeutic targets.

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“…Large animals, such as sheep, present their own challenges: Limited temporal bone volume; Substantial differences in mastoid and ossicular chain structure; Divergent electrophysiological characteristics; High costs; Operational difficulties [3]. Dogs face issues like lengthy research durations, breeding and taming difficulties, aggressive nature, ethical concerns, and high costs [4].…”

Section: Introductionmentioning

confidence: 99%

Current Situation and Progress of Pig Model in Otology Research

Liu,

Ai,

Lin

et al. 2024

JBM

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Pig models are widely used in otological research. The establishment of common pig and miniature pig animal models has opened up new fields in otological research and is also an ideal large otological animal model. This article introduces the applications, current status, progress, advantages, and issues of using pigs as otologic animal models. It summarizes current research on pigs in the fields of hearing disorders, otitis, vertigo, cochlea, gene editing, and tissue engineering, among other otologic and audiological areas. These models are valuable for translating basic medical science into clinical applications. Based on this, platforms have been established for studying deafness and vertigo, cochlear implantation experiments, stem cell and gene therapy, and tissue engineering. These serve as ideal experimental models for the prevention and treatment of ear diseases, pointing toward new directions. This will bolster the promotion and application of pig models in the fields of tissue engineering and gene editing in the future.

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Sheep as a large animal model for cochlear implantation

Cited by 12 publications

References 23 publications

Anatomy of the rabbit inner ear using computed tomography and magnetic resonance imaging

Anatomy of the rabbit inner ear using computed tomography and magnetic resonance imaging

Immediate-Early Modifications to the Metabolomic Profile of the Perilymph Following an Acoustic Trauma in a Sheep Model

Current Situation and Progress of Pig Model in Otology Research

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