The role of MMP-2 and MMP-9 in the metastasis and development of hypopharyngeal carcinoma

The pathological mechanism of colon cancer is very complicated. Therefore, exploring the molecular basis of the pathogenesis of colon cancer and finding a new therapeutic target has become an urgent problem to be solved in the treatment of colon cancer. ATP plays an important role in regulating the progression of tumor cells. P2 × 7 belongs to ATP ion channel receptor, which is involved in the progression of tumors. In this study, we explored the effect and molecular mechanism of ATP-mediated P2 × 7 receptor on the migration and metastasis of colon cancer cells. The results showed that ATP and BzATP significantly increased the inward current and intracellular calcium concentration of LOVO and SW480 cells, while the use of antagonists (A438079 and AZD9056) could reverse the above phenomenon. We found that ATP promoted the migration and invasion of LOVO and SW480 cells and is dose-dependent on ATP concentration (100–300 μM). Similarly, BzATP (10, 50, and 100 μM) also significantly promoted the migration and invasion of colon cancer cells in a concentration-dependent manner. While P2 × 7 receptor antagonists [A438079 (10 μM), AZD9056 (10 μM)] or P2 × 7 siRNA could significantly inhibit ATP-induced colon cancer cell migration and invasion. Moreover, in vivo experiments showed that ATP-induced activation of P2 × 7 receptor promoted the growth of tumors. Furthermore, P2 × 7 receptor activation down-regulated E-cadherin protein expression and up-regulated MMP-2 mRNA and concentration levels. Knocking down the expression of P2 × 7 receptor could significantly inhibit the increase in the expression of N-cadherin, Vimentin, Zeb1, and Snail induced by ATP. In addition, ATP time-dependently induced the activation of STAT3 via the P2 × 7 receptor, and the STAT3 pathway was required for the ATP-mediated invasion and migration. Our conclusion is that ATP-induced P2 × 7 receptor activation promotes the migration and invasion of colon cancer cells, possibly via the activation of STAT3 pathway. Therefore, the P2 × 7 receptor may be a potential target for the treatment of colon cancer.

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“…E-cadherin and MMP-2 are key factors in tumor migration and invasion (Padmanaban et al, 2019;Song et al, 2019).…”

Section: Atp-induced the Activation Of P2 × 7 Receptor Affects The Exmentioning

confidence: 99%

Activation of P2×7 Receptor Promotes the Invasion and Migration of Colon Cancer Cells via the STAT3 Signaling

Zhang

Luo

et al. 2020

Front. Cell Dev. Biol.

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“…In high-risk NBs, tumor cells acquire the ability to degrade extracellular matrix (ECM) components to penetrate the basement membrane of blood vessels and metastasize by activating matrix metalloproteinases (MMPs) [ 7 , 8 ]. MMP-2 and MMP-9, also known as gelatinases, have been shown to play an important role in the invasion and metastasis of many cancers [ 9 , 10 , 11 , 12 , 13 ]. Therefore, targeting cancer cell migration, and thus preventing metastasis formation, is the rationale for more effective anticancer therapy [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Posidonia oceanica (L.) Delile Dampens Cell Migration of Human Neuroblastoma Cells

et al. 2021

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Neuroblastoma (NB) is a common cancer in childhood, and lethal in its high-risk form, primarily because of its high metastatic potential. Targeting cancer cell migration, and thus preventing metastasis formation, is the rationale for more effective cancer therapy against NB. Previous studies have described the leaf extract from Posidonia oceanica marine plant (POE) as an antioxidant, anti-inflammatory agent and inhibitor of cancer cell migration. This study aims to examine the POE anti-migratory role in human SH-SY5Y neuroblastoma cells and the underlying mechanisms of action. Wound healing and gelatin zymography assays showed that POE at early times inhibits cell migration and reduces pro-MMP-2 release into culture medium. By monitoring expression level of key autophagy markers by Western blot assay, a correlation between POE-induced cell migration inhibition and autophagy activation was demonstrated. Cell morphology and immunofluorescence analyses showed that POE induces neurite formation and neuronal differentiation at later times. These results suggest POE might act against cell migration by triggering early nontoxic autophagy. The POE-induced cellular morphological change toward cell differentiation might contribute to prolonging the phytocomplex anti-migratory effect to later times. Overall, these results encourage future in vivo studies to test POE applicability in neuroblastoma treatment.

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“…When overexpressed, these enzymes can damage the integrity of the basement membrane and extracellular matrix by proteolysis, which are important barriers against cell invasion 5 . In particular, MMP-2 and MMP-9 favor the metastasis of tumor cells 6 .…”

Section: Introductionmentioning

confidence: 99%

Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma

Martins

Barros

Ferreira

et al. 2021

Rev. Assoc. Med. Bras.

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OBJECTIVE: This study aims to investigate the association between the immunohistochemical expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and plasma zinc in women with fibroadenoma. METHODS:This cross-sectional study included 37 premenopausal women with fibroadenoma. Waist circumference and body mass index of the participants were measured. Plasma zinc concentrations were determined using atomic flame absorption spectrophotometry. Fragments of breast tissue were fixed and incubated with primary mouse monoclonal antibodies (monoclonal antibodies matrix metalloproteinase -2 -507 and monoclonal antibodies matrix metalloproteinase -9-439). Semi-quantitative analysis of matrix metalloproteinase-2 and matrix metalloproteinase-9 immunoreactivity was performed. Spearman's test and Friedman's test were used for statistical analyses. The p<0.05 were considered statistically significant. RESULTS:The average age of the participants was 32.81±9.51 years. The body mass index and waist circumference values were within the normal range. The mean plasma zinc concentration was 42.73±13.84 μg/dL, with 94.6% inadequacy. A statistically significant difference was found between the positive expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 (p=0.0184). There was no significant correlation between the matrix metalloproteinase expression and the plasma zinc levels.CONCLUSIONS: Women with fibroadenoma had hypozincemia and positive expression of metalloproteinases.

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The role of MMP-2 and MMP-9 in the metastasis and development of hypopharyngeal carcinoma

Cited by 44 publications

References 30 publications

Activation of P2×7 Receptor Promotes the Invasion and Migration of Colon Cancer Cells via the STAT3 Signaling

Activation of P2×7 Receptor Promotes the Invasion and Migration of Colon Cancer Cells via the STAT3 Signaling

Posidonia oceanica (L.) Delile Dampens Cell Migration of Human Neuroblastoma Cells

Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma

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