Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma

Abstract

OBJECTIVE: This study aims to investigate the association between the immunohistochemical expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and plasma zinc in women with fibroadenoma. METHODS:This cross-sectional study included 37 premenopausal women with fibroadenoma. Waist circumference and body mass index of the participants were measured. Plasma zinc concentrations were determined using atomic flame absorption spectrophotometry. Fragments of breast tissue were fixed and incubated with primary mouse monoclonal antibodies (monoclonal antibodies matrix metalloproteinase -2 -507 and monoclonal antibodies matrix metalloproteinase -9-439). Semi-quantitative analysis of matrix metalloproteinase-2 and matrix metalloproteinase-9 immunoreactivity was performed. Spearman's test and Friedman's test were used for statistical analyses. The p<0.05 were considered statistically significant. RESULTS:The average age of the participants was 32.81±9.51 years. The body mass index and waist circumference values were within the normal range. The mean plasma zinc concentration was 42.73±13.84 μg/dL, with 94.6% inadequacy. A statistically significant difference was found between the positive expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 (p=0.0184). There was no significant correlation between the matrix metalloproteinase expression and the plasma zinc levels.CONCLUSIONS: Women with fibroadenoma had hypozincemia and positive expression of metalloproteinases.

Relationship between Trace Elements and Matrix Metalloproteinases 2 and 9 and their Tissue Inhibitors in Medullary Thyroid Carcinoma

“…In our study, Cu, Fe, and Mn showed no relationship with MMPs, their TIMPs and CT, whereas Zn was inversely related with the MMPs of the study, but not with their TIMPs. Similarly to our results, situations of hypozincemia have been reported to be directly related to positive expression of MMPs 2 and 9, but in non-tumorous samples [30]. Moreover, although TIMPs are usually designed to interact with Zn ions in the catalytic domain, thus interfering with enzymatic activity [31], Zn did not correlate with TIMPs in our samples.…”