Brave New (Microbial) World: implications for nasal and sinus disorders

Desrosiers, Martin; Valera, Fabiana Cardoso Pereira

doi:10.1016/j.bjorl.2019.09.001

Cited by 7 publications

(7 citation statements)

References 15 publications

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“…This study indicates the probiotic potential of C. accolens with the potential of this species to help shape a dysbiotic microbiome in the context of CRS by interfering with the growth of MSSA and MRSA in planktonic and biofilm form. Some beneficial nasal bacteria have been evidenced in providing beneficial functions to restore the sinonasal microbiome composition and improving immune health in patients with CRS through direct pathogen inhibition, secretion of a bioactive molecule or nutrient competition [19]. Our results show that C. accolens strains isolated from the sinonasal cavities of non-CRS control patients have antimicrobial activity against MSSA and MRSA strains isolated from the sinonasal cavities of CRS patients.…”

Section: Discussionmentioning

confidence: 66%

Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients

Menberu

Cooksley

et al. 2021

Pathogens

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Corynebacterium accolens is the predominant species of the healthy human nasal microbiota, and its relative abundance is decreased in the context of chronic rhinosinusitis (CRS). This study aimed to evaluate the antimicrobial potential of C. accolens isolated from a healthy human nasal cavity against planktonic and biofilm growth of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) clinical isolates (CIs) from CRS patients. Nasal swabs from twenty non-CRS control subjects were screened for the presence of C. accolens using microbiological and molecular techniques. C. accolens CIs and their culture supernatants were tested for their antimicrobial activity against eight S. aureus and eight MRSA 4CIs and S. aureus ATCC25923. The anti-biofilm potential of C. accolens cell-free culture supernatants (CFCSs) on S. aureus biofilms was also assessed. Of the 20 nasal swabs, 10 C. accolens CIs were identified and confirmed with rpoB gene sequencing. All isolates showed variable antimicrobial activity against eight out of 8 S. aureus and seven out of eight MRSA CIs. Culture supernatants from all C. accolens CIs exhibited a significant dose-dependent antibacterial activity (p < 0.05) against five out of five representative S. aureus and MRSA CIs. This inhibition was abolished after proteinase K treatment. C. accolens supernatants induced a significant reduction in metabolic activity and biofilm biomass of S. aureus and MRSA CIs compared to untreated growth control (p < 0.05). C. accolens exhibited antimicrobial activity against S. aureus and MRSA CIs in both planktonic and biofilm forms and holds promise for the development of innovative probiotic therapies to promote sinus health.

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Section: Discussionmentioning

confidence: 66%

Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients

Menberu

Cooksley

et al. 2021

Pathogens

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“…5,6,17 The structure of the microbiome is an important factor in the recurrence of infections, and its modification with isolated bacterial strain supplementation results in an improvement in patients. 18 Three months of isolation of children not only led to a decrease in upper respiratory tract infections but also improved nasal patency, regardless of the grade of the tonsil hypertrophy. In our study, snoring remained the only symptom dependent on the size of the pharyngeal tonsil.…”

Section: Discussionmentioning

confidence: 99%

Impact of Isolation on Adenoid Size and Symptoms in Preschool Children Who Previously Qualified for Adenoidectomy: A Case–Control Study

et al. 2021

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Objectives: To analyze the role of factors that influence adenoid-related symptoms, and the influence of 3 months of isolation in preschool children who were qualified for adenoidectomy. Methods: This was a cohort study of the impact of 3 months of isolation on children aged 3 to 6 years with adenoid-related symptoms and endoscopically confirmed grade II and III adenoid hypertrophy. The children had previously qualified for adenoidectomy. After 3 months, 141 children were asked about their symptoms, 71 of whom were randomly chosen for medical examination, including endoscopic adenoid examination. Additionally, pre- and post-isolation tympanometry results were analyzed. Results: In our study, significant or mild improvements in health were observed in approximately 73% of the children; 92% of the surveyed parents reported that their children exhibited improved nasal patency, 63% of children discontinued snoring, and 30% of children showed a decrease in snoring. Data collected through surveys correlated with endoscopic findings wherein the size of the adenoid decreased by an average of 5.4%, but the amount of mucus covering the adenoid decreased more significantly in 76% of patients, which may be the main problem determining symptoms reported before isolation. In addition, the amount of residual mucus in the nasopharynx significantly affected the middle ear effusion in adenoid hypertrophy. Conclusions and Relevance: This study found that isolation in preschool children stabilizes the bacterial microbiome of the nose and nasopharynx, thereby having a significant effect not only on the number of recurrent infections of the upper respiratory tract but also on the patency of the nose. The only symptom that depended on the size of the pharyngeal tonsil hypertrophy was snoring. There was a weak correlation between prevalence of infections and adenoid size. During the isolation period, the adenoid size reduced by approximately 5.4% only, statistically more significant in the group with grade III adenoid hypertrophy, but the prevalence of infections rapidly decreased. Residual mucous in the nasopharynx found to be in correlation with middle ear effusion.

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“…In preclinical studies, the organism was well tolerated by sinus epithelium, and at selected concentrations induced production of the anti‐inflammatory factor IL‐10 by peripheral blood mononuclear cells 22 . An early clinical study has been reported to show improvement in symptom and endoscopic scores with twice‐daily use of L. lactis for 14 days in subjects who had failed endoscopic sinus surgery, and hopefully more clinical information will be forthcoming 4 . It is possible that the microbiome of patients who did not respond well to sinus surgery may be more favorably altered by this product.…”

Section: Discussionmentioning

confidence: 99%

“…Low‐pressure, high‐volume saline is commonly used to irrigate the sinonasal cavities, deliver topical medications, or both. Available in the United States and Canada since 2018, Lactococcus lactis W136 (ProbioRinse) is marketed as a probiotic and can be purchased in packet form for topical rinse application in the nose and sinuses 4 . Cho et al co‐cultured different Pseudomonas aeruginosa strains with this commercial L. lactis 5 .…”

Section: Introductionmentioning

confidence: 99%

Microbiomics of irrigation with xylitol or Lactococcus lactis in chronic rhinosinusitis

Lambert

Gill

et al. 2021

Laryngoscope Investig Oto

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Objective Topical sinonasal rinse therapies may alter the local microbiome and improve disease control in chronic rhinosinusitis (CRS). The objective of this study was to examine microbiome changes in post‐surgical CRS patients when rinsing with commercially available products containing xylitol or Lactococcus lactis. Methods A crossover‐type protocol with a washout period was designed. Swab samples from anterior ethmoid cavities of CRS patients were collected prospectively at baseline. Subjects were provided packets containing either L. lactis W136 or xylitol in non‐blinded fashion and instructed to add it to their rinse bottles daily for 28 days, after which another swab was taken. A saline wash‐out period was completed and a third swab taken. A final 28‐day regimen of the opposite product was followed by a final swab. DNA extraction and sequencing of the 16S rRNA gene allowed for global microbiome analysis. Results We enrolled 25 subjects with CRS and 10 controls resulting in 70 adequate samples. Increased detection of Lactococcus was observed after use of L. lactis. No significant trends in alpha or beta diversity as a result of treatment were observed. SNOT‐22 score did not change significantly following treatment with xylitol, L. lactis, or saline. Conclusion We did not detect any major clinical or microbiome‐level effect due to treatment with two topical rinse products. Further research is needed to elucidate their clinical utility and possible probiotic effect. Level of Evidence 3.

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Brave New (Microbial) World: implications for nasal and sinus disorders

Cited by 7 publications

References 15 publications

Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients

Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients

Impact of Isolation on Adenoid Size and Symptoms in Preschool Children Who Previously Qualified for Adenoidectomy: A Case–Control Study

Microbiomics of irrigation with xylitol or Lactococcus lactis in chronic rhinosinusitis

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