Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol

Abstract

Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.

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“…[260] Direct conversion of sclareol to sclareodiol 297,amore advanced intermediate for 296,i se ffected by Hyphozyma roseonigra,b ut this microorganism has al ow tolerance to the antimicrobial activity of (À)-sclareol (294). [261] Aiming at production of 296 independent from plantsourced materials,t he biogenetic sclareol pathway was transferred into E. coli. [262] Thea uthors have shown that the biosynthesis from geranylgeranyl diphosphate (GGPP) involved two diterpene cyclases( Scheme 34).…”

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“…Füre inen mçglichen industriellen Prozess weist Hyphozyma roseonigra aber eine zu geringe Toleranz gegenüber der antimikrobiellen Wirkung von (À)-Sclareol (294)a uf. [261] Mit dem Ziel einer von pflanzlichen Metaboliten unabhängigen Produktion von 296 wurde der Sclareol-Biosyntheseweg auf E. coli transferiert. [262] Es konnte gezeigt werden, dass die pflanzliche Biosynthese von 296 ausgehend von Geranylgeranyldiphosphat (GGPP) von zwei Te rpencyclasen vermittelt wird (Schema 34).…”