First identification of clinical isolate of a Novel “NDM-4” producing Escherichia coli ST405 from urine sample in Pakistan

Qamar, Muhammad Usman; Walsh, Timothy R.; Toleman, Mark A.; Saleem, Sidrah

doi:10.1016/j.bjm.2018.02.009

Cited by 11 publications

(3 citation statements)

References 8 publications

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“…PCR, sequencing, and MLST results showed that all E. coli isolates harbored the bla NDM-4 and mcr-1 genes, and have the sequence type ST405, which is firstly reported in Lebanon and the world. However, the bla NDM-4 E. coli ST405 isolates have already been reported in various studies from hospitalized patients in China and Pakistan [29,30]. Also, the presence of plasmid-mediated mcr-1 E. coli ST405 has been recently described in farmlands in Algeria [31].…”

Section: Discussionmentioning

confidence: 99%

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

Al-Bayssari

Dagher

Hamoui

et al. 2021

J Infect Dev Ctries

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Introduction: The increasing incidence of infections caused by multidrug-resistant bacteria is considered a global health problem. This study aimed to investigate this resistance in Gram-negative bacteria isolated from patients hospitalized in North-Lebanon. Methodology: All isolates were identified using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antibiotic susceptibility testing was achieved using disk diffusion, E-test and Broth microdilution methods. Phenotypic detection of carbapenemase was carried out using the CarbaNP test. RT-PCR, standard-PCR and sequencing were performed to detect resistance genes and oprD gene. Conjugal transfer was carried out between our isolates and Escherichia coli J53 to detect the genetic localization of resistance genes. MLST was conducted to determine the genotype of each isolate. Results: Twenty-three carbapenem-resistant Enterobacterales of which eight colistin-resistant Escherichia coli, and Twenty carbapenem-resistant Pseudomonas aeruginosa were isolated. All isolates showed an imipenem MIC greater than 32 mg/mL with MICs for colistin greater than 2 mg/L for E. coli isolates. All the Enterobacterales isolates had at least one carbapenemase-encoding gene, with E. coli isolates coharboring blaNDM-4 and mcr-1 genes. Moreover, 16/20 Pseudomonas aeruginosa harbored the blaVIM-2 gene and 18/20 had mutations in the oprD gene. MLST revealed that the isolates belonged to several clones. Conclusions: We report here the first description in the world of clinical E. coli isolates coharboring blaNDM-4 and mcr-1 genes, and K. pneumoniae isolates producing NDM-6 and OXA-48 carbapenemases. Also, we describe the emergence of NDM-1-producing E. cloacae in Lebanon. Screening for these isolates is necessary to limit the spread of resistant microorganisms in hospitals.

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Section: Discussionmentioning

confidence: 99%

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

Al-Bayssari

Dagher

Hamoui

et al. 2021

J Infect Dev Ctries

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“…Similarly, another study from Karachi, Pakistan also documented 57% mortality in neonates due to NDM producing bacteria mainly K. pneumoniae [13]. Up till now there are 19 (NDM-1 to NDM-19) variants have been identified in different parts of the world [41]. Among these NDM-1, NDM-5 and NDM-7 are most prevalent variants with a hyper variable region.…”

Section: Class B Carbapenemase Producing K Pneumoniaementioning

confidence: 95%

Antimicrobial Resistance

Qamar¹,

Rasool²,

Jahan³

et al. 2019

Antimicrobials, Antibiotic Resistance, Antibiofilm Strategies and Activity Methods

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Klebsiella pneumoniae (K. pneumoniae) pose an emerging threat to public health sector worldwide. They are one of the potent nosocomial pathogens and cause variety of infections including pneumonia, septicaemia, wound infections, urinary tract infections and catheter-associated infections. From the last two decades, these pathogens are becoming more powerful due to the acquisition of resistomes on different types of plasmids and transposons. There are four main mechanisms of antibacterial resistance such as efflux pump, target alteration, membrane permeability and notably enzymes hydrolysis. K. pneumoniae produce different types of enzymes but most importantly extended spectrum-β-lactamase (ESBL), carbapenemase and metallo-β-lactamase (MBL). K. pneumoniae carbapenemases (KPCs) and New Delhi metallo-β-lactamase (NDM) producing isolates displayed resistance not only against the β-lactam drugs (penicillins, cephalosporins and carbapenems) but also to other classes of antibiotics (aminoglycosides and quinolones). Therapeutic options available to treat serious infections caused by these extensively drug-resistant pathogens are limited to colistin, tigecycline and fosfomycin. Hence, combination therapy has also been recommended to treat such bacteria with clinical side effects, therefore, new treatment regime must be required. Moreover, we are relying on conventional diagnostic tools, however, novel techniques must be required for robust identification of multi-drug-resistant bacteria.

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“…Extraintestinal pathogenic E. coli (ExPEC) is a leading cause of severe infections and has become increasingly resistant to broader classes of antimicrobial agents (Peirano et al, 2013). NDM-producing ST405, phylogenetic group D E. coli is an emerging ExPEC pathogen with sporadic reports from Canada, Denmark, Spain, the United Kingdom, Pakistan, Italy, India, and China (Jakobsen et al, 2014; Peirano et al, 2014; Toleman et al, 2015; Ranjan et al, 2016; Barrado et al, 2018; Qamar et al, 2018; Zhang et al, 2018). bla NDM -carrying IncB/O, IncFII, IncFIA, and IncFIb type plasmids have been identified in ST405 E. coli strains.…”

Section: Introductionmentioning

confidence: 99%

Genotypic and Phenotypic Characterization of Clinical Escherichia coli Sequence Type 405 Carrying IncN2 Plasmid Harboring blaNDM-1

et al. 2019

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We report a bla NDM -carrying ST405 Escherichia coli recovered from the abdominal fluid of a patient in Shandong, China. This strain belonged to the high-risk phylogenetic group D and carried the virulence genes, papG II, papG III, papC , and iroN. In addition to bla NDM-1 , this isolate carried the quinolone resistance gene acc(6′)-Ib and extended-spectrum β-lactamase (ESBL) genes bla CTX-M-15 and bla CTX-M-14 . bla NDM-1 was located on a 41 Kb IncN2 self-transmissible plasmid. The IncN2 plasmid named as pJN24NDM1 was fully sequenced and analyzed. Genome comparative analysis showed that IncN2 plasmids harboring carbapenem-resistance genes possessed conserved backbones and variable accessory regions. Phylogenetic analysis of 87 IncN plasmids based on orthologous genes indicated that 9 IncN2 plasmids fell into one phylogenetic clade, while 4 IncN3 plasmids were in two phylogenetic clades of the 74 IncN1 plasmids. The presence of IncN2 plasmids harboring bla NDM in the high-risk clone ST405 E. coli raises serious concerns for its potential of dissemination.

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First identification of clinical isolate of a Novel “NDM-4” producing Escherichia coli ST405 from urine sample in Pakistan

Cited by 11 publications

References 8 publications

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

Antimicrobial Resistance

Genotypic and Phenotypic Characterization of Clinical Escherichia coli Sequence Type 405 Carrying IncN2 Plasmid Harboring blaNDM-1

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