Genotypic and Phenotypic Characterization of Clinical Escherichia coli Sequence Type 405 Carrying IncN2 Plasmid Harboring blaNDM-1

Hao, Yingying; Shao, Chunhong; Geng, Xu; Bai, Yuanyuan; Jin, Yan; Lu, Zhiming

doi:10.3389/fmicb.2019.00788

Cited by 9 publications

(10 citation statements)

References 33 publications

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“…BLASTp search using the amino acid sequence of RepA SGI1 showed 10–60% similarities to IncN RepA proteins, depending on the subgroup of IncN family. RepA SGI1 significantly differed (<10% identity) from the RepA proteins of IncN1 plasmids ( 43 ), and appeared much more similar (50–60% identity) to those of IncN2 group ( 42 , 41 ) and pN- Cit classified as IncN3 ( 44 ). The phylogenetic reconstruction based on the RepA proteins did not support the separation of IncN2 and IncN3 groups, while IncN1 was clearly distinct from them and possibly forms a different family.…”

Section: Resultsmentioning

confidence: 93%

“…The repA transcript was previously reported to be one of the less abundant mRNAs synthesized from a chromosomally integrated island ( 24 ) and it was not clear whether this gene is functional at all. Pfam search indicated that the putative RepA protein of SGI1 belongs to the RepA_C-domain-containing proteins and further comparative analysis suggested that it is related to the replication initiator proteins of the broad host range plasmids of IncN family ( 42 ) ( Supplementary Figure S1 ). BLASTp search using the amino acid sequence of RepA SGI1 showed 10–60% similarities to IncN RepA proteins, depending on the subgroup of IncN family.…”

Section: Resultsmentioning

confidence: 99%

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IncC helper dependent plasmid-like replication of Salmonella Genomic Island 1

Szabó

Murányi

Kiss

2021

Nucleic Acids Research

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The Salmonella genomic island 1 (SGI1) and its variants are mobilized by IncA and IncC conjugative plasmids. SGI1-family elements and their helper plasmids are effective transporters of multidrug resistance determinants. SGI1 exploits the transfer apparatus of the helper plasmid and hijacks its activator complex, AcaCD, to trigger the expression of several SGI1 genes. In this way, SGI1 times its excision from the chromosome to the helper entry and expresses mating pore components that enhance SGI1 transfer. The SGI1-encoded T4SS components and the FlhDC-family activator proved to be interchangeable with their IncC-encoded homologs, indicating multiple interactions between SGI1 and its helpers. As a new aspect of this crosstalk, we report here the helper-induced replication of SGI1, which requires both activators, AcaCD and FlhDCSGI1, and significantly increases the stability of SGI1 when coexists with the helper plasmid. We have identified the oriVSGI1 and shown that S004-repA operon encodes for a translationally coupled leader protein and an IncN2/N3-related RepA that are expressed under the control of the AcaCD-responsive promoter PS004. This replicon transiently maintains SGI1 as a 4–8-copy plasmid, not only stabilizing the island but also contributing to the fast displacement of the helper plasmid.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

IncC helper dependent plasmid-like replication of Salmonella Genomic Island 1

Szabó

Murányi

Kiss

2021

Nucleic Acids Research

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“…One additional difference between the two plasmids was the presence of Tn 5403 transposon in p47733_OXA-181. The Tn 5403 transposon has been previously found in bla NDM– 1 -positive IncN2 plasmids, like plasmid pJN24NDM1 characterized from a ST405 E. coli from China ( Hao et al, 2019 ). On the other hand, plasmid p50595_OXA-181 was almost identical to plasmids p1-Ec-BERN-042 (100% coverage, 99.99% identity; GenBank accession no.…”

Section: Resultsmentioning

confidence: 99%

Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals

et al. 2021

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The aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged to “high risk” clones ST147, ST11, and ST15, while the Escherichia coli isolate was assigned to ST167. All isolates expressed resistance against most β-lactams, including carbapenems, while retaining susceptibility to colistin. Furthermore, analysis of WGS data showed that all four isolates co-produced OXA-48- and NDM-type carbapenemases, in different combinations (Kpn47733: blaNDM–5 + blaOXA–181; Kpn50595: blaNDM–1 + blaOXA–181; Kpn51015: blaNDM–1 + blaOXA–244; Eco52418: blaNDM–5 + blaOXA–244). In Kpn51015, the blaOXA–244 was found on plasmid p51015_OXA-244, while the respective gene was localized in the chromosomal contig of E. coli Eco52418. On the other hand, blaOXA–181 was identified on a ColKP3 plasmid in isolate Kpn47733, while a blaOXA–181-carrying plasmid being an IncX3-ColKP3 fusion was identified in Kpn50595. The blaNDM–1 gene was found on two different plasmids, p51015_NDM-1 belonging to a novel IncH plasmid group and p51015_NDM-1 being an IncFK1-FIB replicon. Furthermore, the blaNDM–5 was found in two IncFII plasmids exhibiting limited nucleotide similarity to each other. In both plasmids, the genetic environment of blaNDM–5 was identical. Finally, in all four carbapenemase-producing isolates, a diverse number of additional replicons, some of these associated with important resistance determinants, like blaCTX–M–15, arr-2 and ermB, were identified. In conclusion, this study reports the first description of OXA-244-producing Enterobacterales isolated from Czech hospitals. Additionally, our findings indicated the genetic plurality involved in the acquisition and dissemination of determinants encoding OXA/NDM carbapenemases.

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“…35 IncN2 plasmids were reported in E. coli from human hosts and carried bla NDM-1 gene in Thailand, 36 Australia, 37 and China. 38 IncX1 plasmid was present in two isolates, both are ESBL-producing non-ST131 isolates. IncX1 encoding qnrS1 gene was identified in a previous study in a quinolone-resistant E. coli isolate from animal source.…”

Section: Discussionmentioning

confidence: 98%

Plasmid Replicon Diversity of Clinical Uropathogenic Escherichia coli Isolates from Riyadh, Saudi Arabia

Alqasim¹,

Jaffal²,

Almutairi³

et al. 2022

J. Pure Appl. Microbiol.

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The aim of this study was to identify and compare the plasmid replicons of clinical uropathogenic Escherichia coli (UPEC) isolates, involving extended spectrum β-lactamase (ESBL)-positive and ESBL-negative, E. coli ST131 and non-ST131 and various ST131 subclones. Plasmid replicon typing on 24 clinical UPEC isolates was carried out using polymerase chain reaction-based replicon typing. A statistical analysis was performed to assess the associations between plasmid replicon types and ESBL carriage, and to evaluate the link between ST131 isolates and high replicon carriage. Eight replicons, I1α, N2, Iγ, X1, FIIS, K, FIA, and FII were detected. The FII was the most common replicon identified here. ESBL-positive E. coli isolates were highly associated with I1α, N2, Iγ, X1, and FIIS replicons, while FIA was present only in ESBL-negative group. ST131 isolates were highly associated with I1α and N2 replicons compared to non-ST131. No link was found between replicon carriage and the number or type of ESBLs in E. coli isolates. The diversity observed in replicon patterns of our clinical E. coli isolates indicates that they might be originated from different sources. The presence of replicons reported previously in animal sources suggests a possible transfer of antimicrobial resistance between animal and human bacterial isolates.

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Genotypic and Phenotypic Characterization of Clinical Escherichia coli Sequence Type 405 Carrying IncN2 Plasmid Harboring blaNDM-1

Cited by 9 publications

References 33 publications

IncC helper dependent plasmid-like replication of Salmonella Genomic Island 1

IncC helper dependent plasmid-like replication of Salmonella Genomic Island 1

Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals

Plasmid Replicon Diversity of Clinical Uropathogenic Escherichia coli Isolates from Riyadh, Saudi Arabia

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