Elastin increases biofilm and extracellular matrix production of  Aspergillus fumigatus

Brandão, Ildnay de Souza Lima; Oliveira-Moraes, Heloiza Maria da Silva; Souza-Motta, Cristina Maria de; Oliveira, Neiva Tinti de; Magalhães, Oliane Maria Correia

doi:10.1016/j.bjm.2017.10.004

Cited by 9 publications

(5 citation statements)

References 43 publications

(86 reference statements)

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“…What is the role of host DNA and proteins in the organization of the Aspergillus matrix? The addition of exogenous material (DNA and proteins) resulted in greater structural integrity of the carbohydrate matrix (541). What is the exact role of the biofilm in the formation of the granuloma?…”

Section: Some Unexplored Biological Features Of a Fumigatusmentioning

confidence: 99%

Aspergillus fumigatus and Aspergillosis in 2019

2019

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SUMMARY Aspergillus fumigatus is a saprotrophic fungus; its primary habitat is the soil. In its ecological niche, the fungus has learned how to adapt and proliferate in hostile environments. This capacity has helped the fungus to resist and survive against human host defenses and, further, to be responsible for one of the most devastating lung infections in terms of morbidity and mortality. In this review, we will provide (i) a description of the biological cycle of A. fumigatus; (ii) a historical perspective of the spectrum of aspergillus disease and the current epidemiological status of these infections; (iii) an analysis of the modes of immune response against Aspergillus in immunocompetent and immunocompromised patients; (iv) an understanding of the pathways responsible for fungal virulence and their host molecular targets, with a specific focus on the cell wall; (v) the current status of the diagnosis of different clinical syndromes; and (vi) an overview of the available antifungal armamentarium and the therapeutic strategies in the clinical context. In addition, the emergence of new concepts, such as nutritional immunity and the integration and rewiring of multiple fungal metabolic activities occurring during lung invasion, has helped us to redefine the opportunistic pathogenesis of A. fumigatus.

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Section: Some Unexplored Biological Features Of a Fumigatusmentioning

confidence: 99%

Aspergillus fumigatus and Aspergillosis in 2019

2019

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“…To test this hypothesis and according to the results of AFM roughness analysis, hydrophobicity of hyphal biofilm was estimated using hydrophobic fluorescent silica microparticles. 28,51 Indeed, incubation of NGs altered the hydrophobicity of formed biofilm (Figure S14). Higher silica bead absorption was observed for the control sample, in comparison to lower absorption of particles on the fungal cells treated with empty NGs, especially those comprised from PG-BA polymers.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In contrast, to control samples, biofilms incubated with empty NGs float in the medium without spontaneous attachment to the polystyrene surface (data not are shown). To test this hypothesis and according to the results of AFM roughness analysis, hydrophobicity of hyphal biofilm was estimated using hydrophobic fluorescent silica microparticles. , …”

Section: Resultsmentioning

confidence: 99%

“…Spore suspension of A. fumigatus (100 μL, 2 × 10 5 s/mL) was mixed with RPMI 1640 medium (200 μL) and incubated for 24 h at 37 °C. After that, the medium was exchanged to contain 1 mg/mL empty or of AmB-loaded NGs (2 × MIC) and incubated for further 24 h. Samples were further washed with 0.2 M KNO 3 solution (pH 6.5) and treated with 1:1 v/v suspension of fluorescent orange sulfate-modified latex microspheres (0.5 × 10 9 particles/mL, 0.5 μm, Sigma-Aldrich, Germany) in the KNO 3 solution as described previously 28 and incubated for 30 min at RT. After that, biofilms were washed and analyzed by fluorescence microscopy (exc.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Engineering Nanogels for Drug Delivery to Pathogenic Fungi Aspergillus fumigatus by Tuning Polymer Amphiphilicity

Horvat

Böjte

et al. 2020

Biomacromolecules

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Invasive aspergillosis is a serious threat to immunodeficient and critically ill patients caused mainly by the fungus Aspergillus fumigatus. Here, poly(glycidol)-based nanogels (NGs) are proposed as delivery vehicles for antifungal agents for sustained drug release. NGs are formed by simple self-assembly of random copolymers, followed by oxidative cross-linking of thiol functionalities. We investigate the impact of copolymer amphiphilicity on NG interaction with mature fungal hyphae in order to select the optimal drug delivery system for model antifungal drug amphotericin B. The results show that drug-loaded NGs decrease minimal inhibitory concentration (MIC) for around four times and slow down the fungal biofilm synthesis at concentrations lower than MIC. Our results suggest that amphiphilicity of nanoparticle's polymer matrix is an important factor in understanding the action of nanocarriers toward fungal cells and should be considered in the development of nanoparticle-based antifungal therapy.

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“…Next, the biofilms were fixed overnight using 2.5% glutaraldehyde suspension; the coverslips were further rinsed three times using sterile PBS and were dehydrated using a series of ethanol gradients, respectively (50%, 70%, 80%, 90%, 95%) for 15 min. Then, the coverslips were dehydrated twice using 100% ethanol for 20 min (Brandão et al 2018 ). The coverslips were dried by a vacuum freeze dryer (INDEP Mini-E) and were observed by scanning electron microscope (SEM) at a magnification of × 4000 (GeminiSEM 500).…”

Section: Methodsmentioning

confidence: 99%

Effect of L-HSL on biofilm and motility of Pseudomonas aeruginosa and its mechanism

Tang,

Lin,

Yao

et al. 2024

Appl Microbiol Biotechnol

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Pseudomonas aeruginosa (P. aeruginosa) biofilm formation is a crucial cause of enhanced antibiotic resistance. Quorum sensing (QS) is involved in regulating biofilm formation; QS inhibitors block the QS signaling pathway as a new strategy to address bacterial resistance. This study investigated the potential and mechanism of L-HSL (N-(3-cyclic butyrolactone)-4-trifluorophenylacetamide) as a QS inhibitor for P. aeruginosa. The results showed that L-HSL effectively inhibited the biofilm formation and dispersed the pre-formed biofilm of P. aeruginosa. The production of extracellular polysaccharides and the motility ability of P. aeruginosa were suppressed by L-HSL. C. elegans infection experiment showed that L-HSL was non-toxic and provided protection to C. elegans against P. aeruginosa infection. Transcriptomic analysis revealed that L-HSL downregulated genes related to QS pathways and biofilm formation. L-HSL exhibits a promising potential as a therapeutic drug for P. aeruginosa infection. Key points • Chemical synthesis of N-(3-cyclic butyrolactone)-4-trifluorophenylacetamide, named L-HSL. • L-HSL does not generate survival pressure on the growth of P. aeruginosa and can inhibit the QS system. • KEGG enrichment analysis found that after L-HSL treatment, QS-related genes were downregulated.

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Elastin increases biofilm and extracellular matrix production of Aspergillus fumigatus

Cited by 9 publications

References 43 publications

Aspergillus fumigatus and Aspergillosis in 2019

Aspergillus fumigatus and Aspergillosis in 2019

Engineering Nanogels for Drug Delivery to Pathogenic Fungi Aspergillus fumigatus by Tuning Polymer Amphiphilicity

Effect of L-HSL on biofilm and motility of Pseudomonas aeruginosa and its mechanism

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