Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Moghannem, Saad A.; Farag, Mohamed; Shehab, Amr M.; Azab, Mohamed Salah

doi:10.1016/j.bjm.2017.05.012

Cited by 46 publications

(19 citation statements)

References 33 publications

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“…To be more precise, R. minuta’ CRMEP was stimulated by the highest quantity of soybean powder. The same result was obtained by Moghannem, Farag, Shehab, & Azab, 2018 who stated that as carbon and protein source gradually climbed up to the highest points, EPS production sharply rose.…”

Section: Resultssupporting

confidence: 83%

Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658

Samadlouie

Jahanbin

jalali

2020

Food Science & Nutrition

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Several strains of microorganism are capable of converting carbohydrates into extracellular polysaccharide. The preset research is a first effort made to optimize extracellular polysaccharide (CRMEP) by Rhodotorula minuta ATCC 10658 using one factor at time and response surface methods. One factor at time was applied in the initial screening of substrates prior to optimization study. Of all the substrates examined, starch as carbon source and defatted soy bean powder as protein source were discovered to be best for CRMEP production. Response surface analysis revealed that 15 g/L starch and 30g/L defatted soy bean powder were the optimal chemical conditions. The model predicted 13.22 g/L for CRMEP, which went along with the experimentally observed result. Purification of CRMEP by anion‐exchange column of DEAE‐cellulose yielded RMEP. Structural investigation indicated that the main chain of RMEP was composed of (1 → 3) and (1 → 4)‐linked mannopyranosyl residues, with branches attached to O‐6 of some (1 → 3)‐linked mannopyranosyl residues. The branches were composed of Glcp‐(1 → residues.

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Section: Resultssupporting

confidence: 83%

Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658

Samadlouie

Jahanbin

jalali

2020

Food Science & Nutrition

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“…In terms of flocculant production by microorganisms, current research is mostly conducted at the laboratory scale, while the mass production of flocculants is still beyond expectations [ 187 , 188 ]. The mass production of microorganism-based bioflocculant requires abundant and a continuous supply of cultivation medium [ 189 ].…”

Section: Limitations and Future Challengesmentioning

confidence: 99%

“…The mass production of microorganism-based bioflocculant requires abundant and a continuous supply of cultivation medium [ 189 ]. Currently, the cultivation of flocculant-producing microorganisms still utilizes factory-made medium (conventional chemicals) as nutrients [ 187 , 188 ]. It is suggested that the mass production of microorganism-based bioflocculants should consider the utilization of waste products [ 190 , 191 ] or local resources as a growing medium [ 190 , 192 ].…”

Section: Limitations and Future Challengesmentioning

confidence: 99%

Challenges and Opportunities of Biocoagulant/Bioflocculant Application for Drinking Water and Wastewater Treatment and Its Potential for Sludge Recovery

Kurniawan

Abdullah

Imron

et al. 2020

IJERPH

147

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The utilization of metal-based conventional coagulants/flocculants to remove suspended solids from drinking water and wastewater is currently leading to new concerns. Alarming issues related to the prolonged effects on human health and further pollution to aquatic environments from the generated nonbiodegradable sludge are becoming trending topics. The utilization of biocoagulants/bioflocculants does not produce chemical residue in the effluent and creates nonharmful, biodegradable sludge. The conventional coagulation–flocculation processes in drinking water and wastewater treatment, including the health and environmental issues related to the utilization of metal-based coagulants/flocculants during the processes, are discussed in this paper. As a counterpoint, the development of biocoagulants/bioflocculants for drinking water and wastewater treatment is intensively reviewed. The characterization, origin, potential sources, and application of this green technology are critically reviewed. This review paper also provides a thorough discussion on the challenges and opportunities regarding the further utilization and application of biocoagulants/bioflocculants in water and wastewater treatment, including the importance of the selection of raw materials, the simplification of extraction processes, the application to different water and wastewater characteristics, the scaling up of this technology to a real industrial scale, and also the potential for sludge recovery by utilizing biocoagulants/bioflocculants in water/wastewater treatment.

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“…By studying the mutual factor interactions among the variables over various values in a statistically effective manner, RSM simplifies the process optimization based on general statistics principles. 29 Due to the accuracy of the experiment, the central composite design (CCD) is applied frequently in RSM. By finding out the mutual influence and comprehensive effects of the main variables, informative results can be obtained.…”

Section: Introductionmentioning

confidence: 99%

Development of a New Bioprocess for Clean Diosgenin Production through Submerged Fermentation of an Endophytic Fungus

et al. 2021

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Diosgenin is used widely to synthesize steroidal hormone drugs in the pharmaceutical industry. The conventional diosgenin production process, direct acid hydrolysis of the root of Dioscorea zingiberensis C. H. Wright (DZW), causes large amounts of wastewater and severe environmental pollution. To develop a clean and effective method, the endophytic fungus Fusarium sp. CPCC 400226 was screened for the first time for the microbial biotransformation of DZW in submerged fermentation (SmF). Statistical design and response surface methodology (RSM) were implemented to develop the diosgenin production process using the Fusarium strains. The environmental variables that significantly affected diosgenin yield were determined by the two-level Plackett–Burman design (PBD) with nine factors. PBD indicates that the fermentation period, culture temperature, and antifoam reagent addition are the most influential variables. These three variables were further optimized using the response surface design (RSD). A quadratic model was then built by the central composite design (CCD) to study the impact of interaction and quadratic effect on diosgenin yield. The values of the coefficient of determination for the PBD and CCD models were all over 0.95. P -values for both models were 0.0024 and <0.001, with F -values of ∼414 and ∼2215, respectively. The predicted results showed that a maximum diosgenin yield of 2.22% could be obtained with a fermentation period of 11.89 days, a culture temperature of 30.17 °C, and an antifoam reagent addition of 0.20%. The experimental value was 2.24%, which was in great agreement with predicted value. As a result, over 80% of the steroidal saponins in DZW were converted into diosgenin, presenting a ∼3-fold increase in diosgenin yield. For the first time, we report the SmF of a Fusarium strain used to produce diosgenin through the microbial biotransformation of DZW. A practical diosgenin production process was established for the first time for Fusarium strains. This bioprocess is acid-free and wastewater-free, providing a promising environmentally friendly alternative to diosgenin production in industrial applications. The information provided in the current study may be applicable to produce diosgenin in SmF by other endophytic fungi and lays a solid foundation for endophytic fungi to produce natural products.

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Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Cited by 46 publications

References 33 publications

Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658

Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658

Challenges and Opportunities of Biocoagulant/Bioflocculant Application for Drinking Water and Wastewater Treatment and Its Potential for Sludge Recovery

Development of a New Bioprocess for Clean Diosgenin Production through Submerged Fermentation of an Endophytic Fungus

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