Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp.

Abstract

Plant Growth Promoting Rhizobacteria (PGPR) have different mechanisms of action in the development of plants, such as growth promotion, production of phytohormones and antibiotic substances and changes in root exudates. These help to control plant diseases. In order to evaluate the potential of microorganisms in the control of Meloidogyne javanica and Ditylenchus spp., five rhizobacteria isolated from rhizosphere of garlic cultivated in the Curitibanos (SC) region were tested. Hatching chambers were set on Petri dishes, in which were added 10 mL of bacterial suspension and 1 mL of M. javanica eggs suspension, at the rate of 4500, on the filter paper of each chamber. The same procedure was performed with 300 juvenile Ditylenchus spp. The experimental design was completely randomized, with four replications. The evaluations were performed every 72 h for nine days. The antagonized population of nematodes was determined in Peters counting chamber, determining the percentage hatching (for M. javanica) and motility (for Ditylenchus spp). Isolates CBSAL02 and CBSAL05 significantly reduced the hatching of M. javanica eggs (74% and 54.77%, respectively) and the motility of Ditylenchus spp. (55.19% and 53.53%, respectively) in vitro. Isolates were identified as belonging to the genera Pseudomonas (CBSAL05) and Bacillus (CBSAL02).

The Use of Essential Oil and Hydrosol Extracted from Cuminum cyminum Seeds for the Control of Meloidogyne incognita and Meloidogyne javanica

“…However, the persistence and efficacy of the nematicides still in use are reduced due to enhanced degradation [ 3 , 4 ]. Researchers keep trying to find new molecules effective against plant parasitic nematodes [ 5 , 6 , 7 , 8 , 9 ]. Isolated phytochemicals from different plant species have been used as pesticides themselves or have served as model compounds for synthetic agrochemicals by the industry [ 10 ].…”

Intergrated Metagenomics and Metabolomics Analysis Discovers Nematicidal Microbes, Enzymes and Metabolites From the Plant Rhizosphere Microbiota

“…and Bacillus spp. could significantly reduce the hatching of eggs, the numbers of second stage juvenile of Meloidogyne and root galls [6,[59][60][61]. Escherichia coli secreted small molecules including indole, indole-3-carboxaldehyde and indole-3-acetic acid, as virulence factors to kill nematode [62].…”

“…Many rhizosphere microorganisms have been isolated and selected as biological agents for controlling root-knot nematodes [5][6][7]. However, application of microbial agents also raised a novel problem that is the control effect of microbial agents are often unstable in the field [8].…”

Intergrated metagenomics and metabolomics analysis discovers nematicidal microbes, enzymes and metabolites from the plant rhizosphere microbiota

“…Many microorganisms have been isolated and selected as biological agents for controlling root-knot nematodes [9][10][11]. However, the control effect of microbial agents are often unstable in field [12].…”