Genome sequence of Streptomyces mangrovisoli MUSC 149 T isolated from intertidal sediments

Ser, Hooi‐Leng; Tan, Wen Si; Mutalib, Nurul Syakima Ab; Yin, Wai Fong; Chan, Kok‐Gan; Goh, Bey Hing; Lee, Learn‐Han

doi:10.1016/j.bjm.2017.01.013

Cited by 20 publications

(21 citation statements)

References 15 publications

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“…Before DNA library preparation with Nextera ™ DNA Sample Preparation kit (Nextera, USA), genomic DNA quality was checked with Nano-Drop spectrophotometer (Thermo Scientific, Waltham, MA, USA) and a Qubit version 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA). Subsequently, DNA library quality was examined with Bioanalyzer 2100 high sensitivity DNA kit (Agilent Technologies, Palo Alto, CA) [44][45][46] . Whole genome shotgun project of MUSC 1J T was conducted on an Illumina MiSeq platform with MiSeq Reagent Kit 2 (2 × 250 bp; Illumina Inc., Madison, WI, USA).…”

Section: Data Descriptionmentioning

confidence: 99%

Genome sequence of bioactive streptomycete isolated from mangrove forest in East Malaysia, Streptomyces monashensis MUSC 1JT

Ser¹,

Law²,

Tan³

et al. 2019

Prog Drug Discov Biomed Sci

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Members of Streptomyces are known to be prolific producers of bioactive compounds, including antibacterials, antioxidants, neuroprotective agents as well as immunomodulatory compounds. Isolated from East Malaysia, Streptomyces monashensis MUSC 1JT (=DSM 103626T =MCCC 1K03221T) was initially described as a novel streptomycete using a polyphasic approach. Based on phylogenetic analyses, the closely strains related to MUSC 1JT were identified as Streptomyces corchorusii DSM 40340T (98.7 %), Streptomyces olivaceoviridis NBRC 3066T (98.7 %), Streptomyces canarius NBRC 13431T (98.6 %), and Streptomyces coacervatus AS-0823T (98.4 %). Seven day fermentative extracts of MUSC 1JT exhibited potent antioxidant activity and significant cytotoxic activity against colon cancer cell lines, thus the strain was selected for whole genome sequencing. The genome size of MUSC 1JT is described to be 10.3 Mbps with G + C content of 71.50%. Based on antiSMASH analysis, the strain possessess great genomic potential, having fifty nine biosynthetic gene clusters related to production of secondary metabolites and antibiotics. Therefore, these results serve a foundation for further in-depth investigation to harness its bioactive potential for the development of highly valuable pharmaceutical products.

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Section: Data Descriptionmentioning

confidence: 99%

Genome sequence of bioactive streptomycete isolated from mangrove forest in East Malaysia, Streptomyces monashensis MUSC 1JT

Ser¹,

Law²,

Tan³

et al. 2019

Prog Drug Discov Biomed Sci

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show abstract

“…Genomic DNA quality was evaluated with NanoDrop spec-trophotometer (Thermo Scientific, Waltham, MA, USA) and a Qubit version 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA). Subsequently, DNA library was prepared using Nextera ™ DNA Sample Preparation kit (Nextera, USA) and the quality of DNA library was checked with Bioanalyzer 2100 high sensitivity DNA kit (Agilent Technologies, Palo Alto, CA) [24] . Whole genome shotgun project of MUSC 119 T The assembled genome was annotated using Rapid Annotation using Subsystem Technology (RAST) [25] .…”

Section: Data Descriptionmentioning

confidence: 99%

Whole genome sequence of Streptomyces humi strain MUSC 119T isolated from intertidal soil

Ser¹,

Tan²,

Yin³

et al. 2019

Prog Drug Discov Biomed Sci

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Over the past few decades, microorganisms have made major contribution in natural product research, particularly those from the genus Streptomyces. Streptomyces humi MUSC 119T was previously isolated as novel streptomycete from mangrove soil in Malaysia. During the screening programme for bioactive strains, this strain was discovered to possess antioxidant activity – scavenging and reducing accumulation of free radicals in biochemical assays. Consequently, whole genome sequencing was performed to evaluate genomic potential of the strain. Based on our analysis, the genome size of MUSC 119T is described to be 10.01 Mbps with G + C content of 71.80%. Based on antiSMASH analysis, the strain possess great genomic potential, having nine biosynthetic gene clusters displaying high similarities to known gene clusters. These findings indicates that mangrove Streptomyces species like MUSC 119T may potentially play an important role in drug development process, while the availability of its whole genome sequences allows further manipulation to isolate and identify compound of interest.

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“…Genomic DNA of Vibrio sp. SALL 6 was extracted using Masterpure TM DNA purification kit (Epicentre, Il lumina Inc., Madison, WI, USA) before performing RNase (Qiagen, USA) treatment [29,30] . The DNA quality was quantified using NanoDrop spectrophotometer (Thermo Scientific, Waltham, MA, USA) and a Qubit version 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA).…”

Section: Data Descriptionmentioning

confidence: 99%

Genome sequence of Vibrio sp. SALL 6 isolated from shellfish

Letchumanan¹,

Ser²,

Tan³

et al. 2019

Prog Micobes Mol Biol

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Members of the Vibrionaceae family are well known as foodborne pathogen that cause hazard to human in many forms of clinical infection and also affecting aquaculture via infection to livestock. This pathogen has caused seafood associated gastroenteritis cases in many countries including United States, Asian, and South East Asian countries. Antibiotics are usually used as prophylactic and therapeutic to manage the rising Vibrio infections, however, this in turn led to emergence of antibiotic resistant strains in the environments. Vibrio sp. SALL 6 isolated from shellfish was selected for genome sequencing to further explore its antimicrobial traits. Here, a high-quality genome sequence of Vibrio sp. SALL 6 is reported, while its genome reveals a potential for future antibiotic resistance managements.

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Genome sequence of Streptomyces mangrovisoli MUSC 149 T isolated from intertidal sediments

Cited by 20 publications

References 15 publications

Genome sequence of bioactive streptomycete isolated from mangrove forest in East Malaysia, Streptomyces monashensis MUSC 1JT

Genome sequence of bioactive streptomycete isolated from mangrove forest in East Malaysia, Streptomyces monashensis MUSC 1JT

Whole genome sequence of Streptomyces humi strain MUSC 119T isolated from intertidal soil

Genome sequence of Vibrio sp. SALL 6 isolated from shellfish

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