Rapid bacterial identification by MALDI-TOF MS directly from blood cultures and rapid susceptibility testing: A simple approach to reduce the turnaround time of blood cultures

Barth, Patricia Orlandi; Roesch, Eliane Würdig; Lutz, Larissa; Souza, Ândrea Celestino de; Goldani, Luciano Zubaran; Pereira, Dariane Castro

doi:10.1016/j.bjid.2022.102721

Cited by 7 publications

(3 citation statements)

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“…Early appropriate antibiotic therapy is necessary to improve prognosis of bloodstream infections, as the median survival rate decreases by 7.6% per hour of delay in implementing proper treatment ( Kumar et al, 2006 , 2009 ; Perez and Bonomo, 2019 ). In fact, the same bacterial pellet used for DOT-MGA directly from blood samples, can be used for the species identification by MALDI-TOF allowing to obtain both the identification and the susceptibility of the pathogen causing sepsis in the same day of the blood culture positivity ( Barth, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF

et al. 2022

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IntroductionInfections caused by multidrug-resistant microorganisms have become increasingly common in hospital environments around the world. Gram-negative bacilli stands out among multidrug-resistant bacteria mostly due to the production of carbapenemase enzymes which lead to resistance to most β-lactam antibiotics including the carbapenems. As a consequence, polymyxins have been reintroduced in the clinic as a last resort to treat infections caused by Gram-negative bacilli resistant to carbapenems. However, the only reliable method to evaluate the susceptibility to polymyxins is the broth microdilution, a laborious and time-consuming technique. Among infections caused by multidrug-resistant bacteria, bloodstream infections are the most worrisome as they can lead to sepsis and septic shock with high mortality rates.ObjectiveConsidering the severity of sepsis and the need for a treatment guided for the susceptibility test in vitro, this work aimed to evaluate a rapid method of polymyxins susceptibility either from colonies grown on agar or directly from positive blood culture bottles using the technology of MALDI-TOF.MethodsThe method was based on the “direct on target microdroplets growth assay” (DOT-MGA) originally developed by Idelevich and collaborators with some modifications (Adapted DOT-MGA). Isolates of Enterobacterales and non-fermenting Gram-negative bacilli resistant to carbapenems were obtained from patients attending a tertiary care hospital in southern Brazil and tested as follows: 122 isolates from colonies grown on agar plates and 117 isolates directly from spiked positive blood cultures.ResultsThe adapted DOT-MGA presented 95 and 100% of categorical agreement considering the colonies grown on agar plates and directly from positive blood cultures, respectively.DiscussionThe adapted DOT-MGA test proved to be a reliable technique to evaluate the susceptibility to polymyxins to be used in microbiology laboratories with the MALDI-TOF equipment.

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Section: Discussionmentioning

confidence: 99%

Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF

et al. 2022

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“…Out of the MALDI MS instruments used, Microex of Bruker is the most extensively used instrument for microbial identication. [49][50][51][52][53] Fig. 2 represents the different sample preparation methods that have been in practice for the MALDI MS iden-tication of bacterial isolates.…”

Section: Methods Of Sample Preparationsmentioning

confidence: 99%

“…Out of the MALDI MS instruments used, Microflex of Bruker is the most extensively used instrument for microbial identification. 49–53…”

Section: Maldi Ms Spectrum-based Identification Of Microbial Pathogensmentioning

confidence: 99%

A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

et al. 2023

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Rapid colorimetric polymyxin B microelution directly from positive blood bottles: because patients with serious infections should not have to wait for results of culture-based methodologies

Collar,

Becker,

Moreira

et al. 2024

Eur J Clin Microbiol Infect Dis

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Rapid bacterial identification by MALDI-TOF MS directly from blood cultures and rapid susceptibility testing: A simple approach to reduce the turnaround time of blood cultures

Cited by 7 publications

References 23 publications

Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF

Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF

A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

Rapid colorimetric polymyxin B microelution directly from positive blood bottles: because patients with serious infections should not have to wait for results of culture-based methodologies

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