“…To test the current disease state and a possible scenario of overlapped outbreaks, a multiplexed combination of arboviruses and COVID-19 was used. The WHO recommends multiplexing ZIKV, DENV, and CHIKV as an ideal detection method, and this combination was found to allow virus distinction in possible co-circulation situations ( do Rosario and de Siqueira, 2020 ; WHO, 2016 ). Qualitative multiplex immunoassays using a threshold value to define positive or negative results were demonstrated with randomly spiked samples in PBS (i.e., anti-ZIKV NS1, anti-DENV NS1, anti-CHIKV E2, anti-ZIKV/DENV NS1, anti-ZIKV/CHIKV NS1/E2, anti-DENV/CHIKV NS1/E2, anti-ZIKV/DENV/CHIKV NS1/E2, anti-SARS-CoV-2 RBD, and all biomarkers).…”