Staphylococcus aureus in tonsils of patients with recurrent tonsillitis: prevalence, susceptibility profile, and genotypic characterization

Cavalcanti, Veraluce Paolini; Camargo, Lívia Arantes; Moura, Fabiano Santana; Fernandes, Eduardo Vignoto; Lamaro‐Cardoso, Juliana; Silva, Carla Afonso da; André, Maria Cláudia Porfirio

doi:10.1016/j.bjid.2018.12.003

Cited by 18 publications

(16 citation statements)

References 32 publications

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“…In this study, the percentage of Methicillin Resistant S. aureus (MRSA) isolates among children with tonsillitis was 15.8%. This is higher than studies done in Germany (0.8%) [37], Lahore (5.5%) [39], Japan (0.8%) [40], Brazil (3.3%) [24] and Ethiopia (2.3%) [41], but lower than studies done in the USA (16%) [42], Benin (17.95%) [5] and Uganda (32%) [43]. The variation between the studies might be due to geographical variations, age and child contact to hospitalized patients who could have contracted the MRSA from hospitals.…”

Section: Discussioncontrasting

confidence: 57%

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Bacterial Tonsillitis and Antimicrobial Resistance Profiles Among Children Within Five Years of Age At Hargeisa Group of Hospital, Somaliland: A Cross-Sectional Study

Darod

Melese

Kibret³

et al. 2021

Preprint

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Background Tonsillitis is the third most frequently diagnosed infection in the pediatrics age group around the world. It causes significant morbidity and loss of school attendance in children. The emergence of drug resistance in bacterial tonsillitis is getting higher every year. However, data on the drug resistance profiles of bacterial causes of tonsillitis among children within five years of age is not available in Somaliland.Therefore; this study determined the bacterial causes of tonsillitis and their antimicrobial resistance profiles among children within five years of age at Hargeisa Group of Hospital, Somaliland. Methods A cross-sectional study was conducted from March to July 2020. A total of 374 children within five years of age were included using convenient sampling method. Throat swabs were collected from children, processed and bacterial species were identified using standard bacteriological procedures. Antimicrobial susceptibility was done using disc diffusion method. Data on demographic variables and clinical profiles were collected using structured questionnaires. Logistic regression analysis was computed to identify factors associated with bacterial tonsillitis. P-values < 0.05 were taken as statistically significant. Results The median age of children included in the study was 4 years. Overall, 120(32.1%)(95% CI 27.4–36.8%) of children had culture confirmed bacterial tonsillitis. Of them,23(19.2%) had mixed infections.The most frequent bacterial isolates were Streptococcus pyogenes 78(55%), Staphylococcus aureus 42 (29%) and Streptococcus pneumoniae 10(7%). Isolates revealed 83.3–100 % rate of resistance to ampicillin. S. aureus was resistant to clarithromycin (38%) while 60% of S. pneumoniae isolates were resistant to gentamicin. The overall multidrug resistance (MDR) was 50.4% and 52.6% of S.pyogenes and 60% of S.pneumoniae were MDR. History of tonsillitis(AOR = 0.12; 95% CI = 0.06–0.21), difficulty of swallowing(AOR = 6.99; 95% CI = 3.56–13.73), weight loss (AOR = 0.33; 95% CI = 0.186–0.597) and attending school (AOR = 2.98; 95% CI = 1.64–5.42) were found to be associated with tonsillitis among children within five years of age. Conclusions Bacterial tonsillitis with high degree of ampicillin resistance, mixed infections and MDR isolates are major concerns in children within five years of age at Hargeisa, Somaliland. Therefore, treatment of cases should be guided by regular culture and antimicrobial susceptibility testing to prevent disease consequences and critical drug resistance.

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Section: Discussioncontrasting

confidence: 57%

“…[46], but was higher than studies done in Brazil (24.6%) [24]. On the other hand, the resistance pro le of S. pneumoniae to erythromycin (30%) is similar to a study done in Malaysia, with a rate of (30%) but different from studies done in China (56%, 20%) [47], Lithuania (78.8%) [48] and Ethiopia (12.4%) [49].…”

Section: Discussionmentioning

confidence: 51%

Bacterial Tonsillitis and Antimicrobial Resistance Profiles Among Children Within Five Years of Age At Hargeisa Group of Hospital, Somaliland: A Cross-Sectional Study

Darod

Melese

Kibret³

et al. 2021

Preprint

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“…In the present study, of the 27 S. aureus isolated, were found resistance that are similar to the study developed by Cavalcanti et al 29 , that found 83.6% (53/123) of resistance to penicillin, 26.2% (16/123) to cefoxitin, 16.4% (10/123) to erythromycin, 11.5% (7/123) to tetracycline, 9.8% (6/123) to clindamycin, and 100% of susceptibility to gentamicin.…”

Section: Discussionsupporting

confidence: 92%

Resistance profile from Staphylococcus aureus and Pseudomonas aeruginosa Obtained from tracheostomized children

Almeida

Barros

Ayres

et al. 2020

Preprint

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Background The tracheostomized patients exhibit high risks of bacterial infections, because the tracheal tube acts as a gateway to these microorganisms. The objective was to characterize microbiologically the tracheal secretion of tracheostomized children, to evaluate the biofilm formation, and to study the phenotypic and molecular profile of antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated. Methods The study collected 88 tracheal secretion samples. The material processed by phenotypic tests were performed for bacterial identification. For identification of the biofilm, the Congo red agar test and the plaque microtiter test were used, and the qPCR method was used to resistance verification. Results Were obtained 27 samples of S. aureus and 71 of P. aeruginosa . All S. aureus samples were positive for biofilm formation on Congo red agar test. In antibiogram test, S. aureus showed resistance to seven drugs. Regarding the identification of resistance genes, were amplificated bla Z in 42.8% from S. aureus and mec A in 28.6% of them. Pseudomonas aeruginosa presented resistance to eight drugs. The most frequent chromosomal genes were bla OXA with 66.7% and bla KPC with 58.3%. To plasmidial DNA, was highlighted bla NDM with 58.3% positive. ConclusionThe S. aureus and P. aeruginosa characterization of colonization from lower respiratory tract associated with the use of the device in tracheotomy patients, and the physiology and antimicrobial resistance profile, will help health professionals to choose the most appropriate treatment to be administered in children with tracheotomy, increasing the chances of airway restoration and decannulation.

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“…In the present study, of the 27 S. aureus isolated, were found resistance that are similar to the study developed by Cavalcanti et al 29 30,31 .…”

Section: Discussionsupporting

confidence: 91%

Resistance Profile From Staphylococcus Aureus And Pseudomonas Aeruginosa Obtained From Tracheostomized Children

Almeida

Barros

Ayres

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The tracheostomized patients exhibit high risks of bacterial infections, because the tracheal tube acts as a gateway to these microorganisms. The objective was to characterize microbiologically the tracheal secretion of tracheostomized children, to evaluate the biofilm formation, and to study the phenotypic and molecular profile of antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated. Methods The study collected 88 tracheal secretion samples. The material processed by phenotypic tests were performed for bacterial identification. For identification of the biofilm, the Congo red agar test and the plaque microtiter test were used, and the qPCR method was used to resistance verification. Results Were obtained 27 samples of S. aureus and 71 of P. aeruginosa . All S. aureus samples were positive for biofilm formation on Congo red agar test. In antibiogram test, S. aureus showed resistance to seven drugs. Regarding the identification of resistance genes, were amplificated bla Z in 42.8% from S. aureus and mec A in 28.6% of them. Pseudomonas aeruginosa presented resistance to eight drugs. The most frequent chromosomal genes were bla OXA with 66.7% and bla KPC with 58.3%. To plasmidial DNA, was highlighted bla NDM with 58.3% positive. Conclusion The S. aureus and P. aeruginosa characterization of colonization from lower respiratory tract associated with the use of the device in tracheotomy patients, and the physiology and antimicrobial resistance profile, will help health professionals to choose the most appropriate treatment to be administered in children with tracheotomy, increasing the chances of airway restoration and decannulation.

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Staphylococcus aureus in tonsils of patients with recurrent tonsillitis: prevalence, susceptibility profile, and genotypic characterization

Cited by 18 publications

References 32 publications

Bacterial Tonsillitis and Antimicrobial Resistance Profiles Among Children Within Five Years of Age At Hargeisa Group of Hospital, Somaliland: A Cross-Sectional Study

Bacterial Tonsillitis and Antimicrobial Resistance Profiles Among Children Within Five Years of Age At Hargeisa Group of Hospital, Somaliland: A Cross-Sectional Study

Resistance profile from Staphylococcus aureus and Pseudomonas aeruginosa Obtained from tracheostomized children

Resistance Profile From Staphylococcus Aureus And Pseudomonas Aeruginosa Obtained From Tracheostomized Children

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Staphylococcus aureus in tonsils of patients with recurrent tonsillitis: prevalence, susceptibility profile, and genotypic characterization

Cited by 18 publications

References 32 publications

Bacterial Tonsillitis and Antimicrobial Resistance Profiles Among Children Within Five Years of Age At Hargeisa Group of Hospital, Somaliland: A Cross-Sectional Study

Bacterial Tonsillitis and Antimicrobial Resistance Profiles Among Children Within Five Years of Age At Hargeisa Group of Hospital, Somaliland: A Cross-Sectional Study

Resistance profile from Staphylococcus aureus&nbsp;and Pseudomonas aeruginosa Obtained from tracheostomized children

Resistance Profile From Staphylococcus Aureus And Pseudomonas Aeruginosa Obtained From Tracheostomized Children

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Resistance profile from Staphylococcus aureus and Pseudomonas aeruginosa Obtained from tracheostomized children