Detection of the mec A gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction

Rocchetti, Taisa Trevizani; Martins, Katheryne Benini; Faccioli-Martins, Patrícia Yoshida; Oliveira, Rogério Antônio de; Mondelli, Alessandro Lia; Fortaleza, Carlos Magno Castelo Branco; Cunha, Maria de Lourdes Ribeiro de Souza da

doi:10.1016/j.bjid.2018.02.006

Cited by 27 publications

(22 citation statements)

References 23 publications

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“…Overall, 57.14% of isolated S. aureus possess the mecA gene. These results are consistent with those of Rocchetti et al [37], who reported that 50.6% of S. aureus isolated from blood culture bottles in Brazil had the mecA gene. On the other hand, our results are contrary to those of Sajit Khan et al [38] in South India, who found that 94% of clinical MRSA were carriers of the mecA gene.…”

Section: Discussionsupporting

confidence: 93%

“…This difference could be explained by a low transfer of the mecA gene within S. aureus strains from CHU-Z Abomey-Calavi/Sô-Ava. Furthermore, several studies show that the mecA gene is also present in coagulase-negative staphylococci [37]. It is important that this work can help improve new therapy options to limit the spread of these pathogens.…”

Section: Discussionmentioning

confidence: 99%

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Pathogenicity and Molecular Characterization of Staphylococcus aureus Strains Isolated from the Hospital Environment of CHU-Z Abomey-Calavi/Sô-Ava (Benin)

Socohou

Sina

Dégbey

et al. 2021

BioMed Research International

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Staphylococcus aureus is a major human pathogen present on a third of the healthy population. The bacterium possesses an extensive arsenal of virulence factors. The pathogenicity is linked with S. aureus high plasticity and its exceptional ability to incorporate foreign genetic material. The aim of the present study was to perform molecular characterization of Staphylococcus aureus strains isolated from the clinical environment of the CHU-Z Abomey-Calavi/Sô-Ava. Isolation of Staphylococcus aureus bacterium was performed on Chapman agar. Toxin production by isolated S. aureus strains was investigated using the radial immunoprecipitation technique. A colorimetric assay was used to evaluate Staphylococcus aureus lipase (SA-Lipase) production. Finally, the expression of antibiotic resistance genes and genes encoding toxins production was investigated. Our data suggest that none of the isolated Staphylococcus aureus strains expressed the investigated toxin genes. Interestingly, SA-Lipase was produced by 14.28% of our isolated S. aureus strains. The mecA gene was present in 57.14% of the isolated strains, while PVL and TSST-1 genes were identified in 2.85 and 7.14% of S. aureus, respectively. Significant genetic diversity was observed along the hospital environment S. aureus strains. The present study reveals the level of virulence of S. aureus strains isolated in the different units of CHU-Z Abomey Calavi/Sô-Ava through the production of lipase, PVL, and epidermolysins. The molecular study has favored a genetic characterization within the isolated strains.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Pathogenicity and Molecular Characterization of Staphylococcus aureus Strains Isolated from the Hospital Environment of CHU-Z Abomey-Calavi/Sô-Ava (Benin)

Socohou

Sina

Dégbey

et al. 2021

BioMed Research International

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“…However, these systems are very expensive and are not widely available, especially in low-resource settings. Several molecular techniques, including PCR-based methods, were tested in positive blood culture samples with high sensitivity and specificity within 3 h [ 6 , 7 ]. However, they also require special equipment and well-trained operating personnel.…”

Section: Introductionmentioning

confidence: 99%

Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

et al. 2020

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Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

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“…These tests have proven to be highly accurate in detecting the strains of Staphylococcus sp. possessing mecA gene [5][6][7][8] (91.6-100% concordance with the phenotypic method), resulting in shorter duration of the vancomycin therapy. 9,10 Compared with the standard blood culture, RMTs reported that sensitivities are between 87 and 94% 6,11 ; however, specificity still remains 100%.…”

Section: Introductionmentioning

confidence: 99%

Clinical Impact of BioFire Film Array in Pediatric Sepsis

Moraru

Chatani

Martínez

et al. 2019

J Pediatr Infect Dis

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Objective Rapid molecular tests are important components of antimicrobial stewardship programs (ASP) and for early and accurate diagnosis of sepsis etiology. Their utility in helping providers to de-escalate therapy remains a subject of debate, especially among patients with chronic conditions and indwelling devices. Methods We have conducted a retrospective cohort study of medical records from August 1, 2016 to July 31, 2018, following the implementation at our institution of the BioFire FilmArray blood culture identification panel (BioFire Diagnostics, Salt Lake City, Utah, United States). We have assessed the clinical impact of the assay looking at antimicrobial optimization and time needed to make this change in different pediatric wards. Results Two hundred and twenty-nine patients were included in our analysis. We identified a significant statistical difference between different pediatric wards regarding antimicrobial therapy optimization (p < 0.001) and between the type of therapeutic attitude related to BioFire result, escalation versus de-escalation, or no change (p < 0.03). The likelihood for an infectious disease consult requested upon BioFire result to lead to an antimicrobial regimen optimization was also statistically significant (p < 0.01). The positive BioFire results were associated with antimicrobial escalation in 81.4% of cases, with approximately 75% of changes made within 12 hours of the assay result. Conclusion Our study suggests that BioFire Film Array does not help de-escalating the therapy in patients with chronic disorders. Physician education and level of confidence in the assay remains an essential obstacle in maximizing the test performance in clinical practice. In recent years, the market for more sensitive and comprehensive rapid molecular diagnostic tests has evolved rapidly, requiring effective ASP to optimize the management of infectious diseases.

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Detection of the mec A gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction

Cited by 27 publications

References 23 publications

Pathogenicity and Molecular Characterization of Staphylococcus aureus Strains Isolated from the Hospital Environment of CHU-Z Abomey-Calavi/Sô-Ava (Benin)

Pathogenicity and Molecular Characterization of Staphylococcus aureus Strains Isolated from the Hospital Environment of CHU-Z Abomey-Calavi/Sô-Ava (Benin)

Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Clinical Impact of BioFire Film Array in Pediatric Sepsis

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