The role of microRNAs in enteroviral infections

Wu, Jing; Шен, Ли; Chen, Jian Guo; Xu, Huaxi; Mao, Lingxiang

doi:10.1016/j.bjid.2015.06.011

Cited by 20 publications

(15 citation statements)

References 65 publications

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“…NF-κB signaling-related molecules IRAK2 were found modulated by miR-1246 and miR-320a, and TRAF4 was found modulated by miR-1246 and miR-760-3p in PPRV infected lung of goats in the present study. Interferon (IFN)-mediated pathway is a crucial part of the cellular response against viral infection (Wu et al, 2015). TRAF6, a major element in IFN production (Yoshida et al, 2008) was suppressed by PPRV-induced miR-21-3p and miR-320a in the lung of sheep.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

et al. 2017

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Peste des petits ruminants (PPR) is one of the highly contagious viral disease, characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, primarily affecting sheep and goats. Reports suggested variable host response in goats and sheep and this host response vis-a-vis the expression of microRNAs (miRNAs) has not been investigated. Here, miRNAs were sequenced and proteomics data were generated to identify the role of differentially expressed miRNA (DEmiRNA) in PPR virus (PPRV) infected lung and spleen tissues of sheep and goats. In lungs, 67 and 37 DEmiRNAs have been identified in goats and sheep, respectively. Similarly, in spleen, 50 and 56 DEmiRNAs were identified in goats and sheep, respectively. A total of 20 and 11 miRNAs were found to be common differentially expressed in both the species in PPRV infected spleen and lung, respectively. Six DEmiRNAs—miR-21-3p, miR-1246, miR-27a-5p, miR-760-3p, miR-320a, and miR-363 were selected based on their role in viral infections, apoptosis, and fold change. The target prediction analysis of these six selected DEmiRNAs from the proteome data generated, revealed involvement of more number of genes in lung and spleen of goats than in sheep. On gene ontology analysis of host target genes these DEmiRNAs were found to regulate several immune response signaling pathways. It was observed that the pathways viz. T cell receptor signaling, Rap1 signaling, Toll-like receptor signaling, and B cell receptor signaling governed by DEmiRNAs were more perturbed in goats than in sheep. The data suggests that PPRV-induced miR-21-3p, miR-320a, and miR-363 might act cooperatively to enhance viral pathogenesis in the lung and spleen of sheep by downregulating several immune response genes. The study gives an important insight into the molecular pathogenesis of PPR by identifying that the PPRV—Izatnagar/94 isolate elicits a strong host response in goats than in sheep.

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Section: Discussionmentioning

confidence: 99%

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

et al. 2017

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“…MicroRNAs (miRNAs) were recently put forward as a convincible marker that circulates in the bloodstream [31]. MiRNA is involved in many biological processes in cells and its expression is associated with cancers and other diseases, such as enteroviral infections [32]. Furthermore, miRNA has been considered as a biomarker for diagnosing various diseases in clinical practices [33,34].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Indicating Value of Cardiac Troponin I, Tumor Necrosis Factor-α, Interleukin-18, Mir-1 and Mir-146b for Viral Myocarditis among Children

Wang

Cui

et al. 2016

Cell Physiol Biochem

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Background/Aims: The primary objective of this study is to evaluate the diagnosis effect of serum protein factors and microRNAs for children suffering from viral myocarditis (VMC). Methods: The expression levels of serum cardiac troponin I (cTnI), interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) in both VMC and control groups were examined by using the Elisa Kit. The expression levels of miR-1 and miR-146b were measured through RT-PCR. Subsequently, the Receiver Operating Characteristic (ROC) curves were drawn based on the diagnostic results of VMC. Moreover, the Spearman correlation analysis was carried out to unveil the association between the indicator expression levels and the ultrasonic cardiogram results, including the left ventricular fractional shortening (FS) and left ventricular ejection fraction (EF). Results: It is found that the expression levels between the VMC and control group portrait significant differences with respect to cTnI, IL-18, TNF-α, miR-1 and mIR-146b (P < 0.05). The diagnostic thresholds for cTnI, IL-18, TNF-α were 160.00 ng/L, 2.26 ng/L and 1.14ng/L, respectively. The diagnostic thresholds for miR-1 and miR-146b were 0.75 and 1.27, respectively. Results from the Spearman correlation analysis showed that levels of the miR-1 were negatively correlated with FS and EF, while levels of the cTnI, IL-18, TNF-α and miR-146b were positively correlated with FS and EF. Conclusions: The expression levels of the TNF-α, IL-18 and cTnI and the expression levels of the miR-1 and miR-146b could be used to predict VMC among children and this approach may reinforce the diagnosis of VMC in clinical practices.

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“…Since their discovery in 1993, a range of knowledge has been accumulated, showing the importance of miRNA in cellular physiological and pathological processes, including cell development, metabolism, proliferation, differentiation, and apoptosis (Ebert and Sharp, 2012 ; Yates et al, 2013 ). Over the past few decades, mounting evidence has supported the notion that the miRNAs may serve as key regulators of viral entry and replication (Wu et al, 2015 ). This is perhaps best illustrated by the observation that hsa-miR-122, a liver-specific miRNA, is able to bind with the 5′ UTR of hepatitis C virus (HCV) to stabilize HCV genomic RNA (Jopling et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…To date, it has become increasingly clear that the miRNAs play a pivotal role in EV71 replication by altering the level of cellular miRNAs, which in turn regulates the expression of viral or host genes (Cui et al, 2010 ; Wu et al, 2015 ; Xun et al, 2015 ). Here, it is noteworthy that the downregulation of DGCR8 (DiGeorge critical region 8), an essential cofactor for the biogenesis of miRNAs, results in the inhibition of EV71 infection (Lui et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Host MicroRNA hsa-miR-494-3p Promotes EV71 Replication by Directly Targeting PTEN

Zhao

Xiong²,

Xu³

et al. 2018

Front. Cell. Infect. Microbiol.

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Many cellular processes are driven by spatially and temporally regulated microRNAs (miRNAs)-dependent signaling events. Substantial evidence collected over the years indicates that miRNAs are pivotal regulators that contribute to the initiation and development of EV71-related disorders. Importantly, so far, no clinical trial has been undertaken to address the effect of miRNAs on EV71-related diseases. In this study, we show that EV71 infection results in up-regulation of hsa-miR-494-3p levels, and that EV71-induced hsa-miR-494-3p impacts PI3K/Akt signaling pathway by targeting PTEN. However, very little is known about the relationship between hsa-miR-494-3p and EV71 infection. The overall goal of the study is to get a better insight into whether or not hsa-miR-494-3p is involved in the EV71 infection. We found that the EV71 infection induces cellular apoptosis, and that this process can be counteracted by the over-expression of hsa-miR-494-3p mimics. We also present evidence that cell lines deficient in hsa-miR-494-3p are more sensitive to EV71-induced cell death than the corresponding control cells. Collectively, these findings confirm and extend the pervious observation suggesting that disturbances in miRNAs expression can influence EV71 propagation. In addition, they lend strong support to the ideas that hsa-miR-494-3p-mediated signaling pathway plays an important role in the EV71 replication, and that this may have profound implications on our views on EV71-related diseases.

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The role of microRNAs in enteroviral infections

Cited by 20 publications

References 65 publications

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

Analysis of the Indicating Value of Cardiac Troponin I, Tumor Necrosis Factor-α, Interleukin-18, Mir-1 and Mir-146b for Viral Myocarditis among Children

Host MicroRNA hsa-miR-494-3p Promotes EV71 Replication by Directly Targeting PTEN

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