Analysis of the Indicating Value of Cardiac Troponin I, Tumor Necrosis Factor-α, Interleukin-18, Mir-1 and Mir-146b for Viral Myocarditis among Children

Abstract

Background/Aims: The primary objective of this study is to evaluate the diagnosis effect of serum protein factors and microRNAs for children suffering from viral myocarditis (VMC). Methods: The expression levels of serum cardiac troponin I (cTnI), interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) in both VMC and control groups were examined by using the Elisa Kit. The expression levels of miR-1 and miR-146b were measured through RT-PCR. Subsequently, the Receiver Operating Characteristic (ROC) curves were drawn based on the diagnostic results of VMC. Moreover, the Spearman correlation analysis was carried out to unveil the association between the indicator expression levels and the ultrasonic cardiogram results, including the left ventricular fractional shortening (FS) and left ventricular ejection fraction (EF). Results: It is found that the expression levels between the VMC and control group portrait significant differences with respect to cTnI, IL-18, TNF-α, miR-1 and mIR-146b (P < 0.05). The diagnostic thresholds for cTnI, IL-18, TNF-α were 160.00 ng/L, 2.26 ng/L and 1.14ng/L, respectively. The diagnostic thresholds for miR-1 and miR-146b were 0.75 and 1.27, respectively. Results from the Spearman correlation analysis showed that levels of the miR-1 were negatively correlated with FS and EF, while levels of the cTnI, IL-18, TNF-α and miR-146b were positively correlated with FS and EF. Conclusions: The expression levels of the TNF-α, IL-18 and cTnI and the expression levels of the miR-1 and miR-146b could be used to predict VMC among children and this approach may reinforce the diagnosis of VMC in clinical practices.

Comparative transcriptomic analysis of porcine peripheral blood reveals differentially expressed genes from the cytokine–cytokine receptor interaction pathway related to health status

“…It plays key roles in inflammatory and immune responses and has significant influence on the pathogenesis of diseases (Lukens et al 2012). Up-regulated serum IL-18 level was also reported in sepsis (Feng et al 2016), asthma (Xu et al 2017), and viral myocarditis (Wang et al 2016) and was reported to be involved in intestinal inflammation (Sivakumar et al 2002;Siegmund 2010) and colitis (Kanai et al 2001;Ishikura et al 2003).…”

Upregulated Serum MiR-146b Serves as a Biomarker for Acute Ischemic Stroke

“…There is no significance of the expression of serum miR-146b among 5 AIS groups in this study (data not shown), which may suggest that miR-146b may be a biomarker for AIS but not for separating subtypes of AIS. In fact, miR-146b is one of the most-studied miRs [32,33], and especially in the vascular smooth muscle cell (VSMCs) proliferation, differentiation, and phenotypic switching. In cardiovascular system, miR-146b is not only important for heart and vascular development but also plays an essential role in cardiac pathological factors, such as hypertrophy and ischemia, and has been regarded as a new therapeutic target for vascular disease [34][35][36].…”

The Role of Non-coding RNAs in Viral Myocarditis

“…MiR-1 decreased significantly in VMC, which was negatively correlated with the left ventricular shortening fraction and left ventricular ejection fraction. MiR-146b was significantly up-regulated in VMC, which was positively correlated with those two indexes (Wang D. et al, 2016 ). Therefore, miR-1 and miR-146b may be potential disease markers of VMC.…”