A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli

Bagheri, Samane; Gargari, Seyed Latif Mousavi; Rasooli, Iraj; Nazarian, Shahram; Alerasol, Masoome

doi:10.1016/j.bjid.2013.07.012

Cited by 12 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, ETEC is one of the most common bacteria responsible for diarrhea in different parts of the world (18). The ETEC bacteria threaten the lives of many people, especially children under five years old all around the world and also in Iran (4,5,19).…”

Section: Discussionmentioning

confidence: 99%

Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates

Esfandiari

Amani

Fouladi

et al. 2016

Arch Clin Infect Dis

Self Cite

View full text Add to dashboard Cite

Background: Enterotoxigenic Escherichia coli (ETEC) is the most common agent, which causes diarrhea. ETEC is colonized along the cells and produces enterotoxins leading to diarrhea. Different detection methods have been utilized for detection of ETEC heat Labile Toxin (LT) toxins or respective genes. These methods have disadvantages such as high costs and labor time and limitations in handling many samples simultaneously.

show abstract

Section: Discussionmentioning

confidence: 99%

Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates

Esfandiari

Amani

Fouladi

et al. 2016

Arch Clin Infect Dis

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, we fused cstH and eltB coding sequences with a suitable (EAAAK)4 linker, and (EAAAK)4 sequences were introduced among different domains for more flexibility and efficient separation. Our successful experience of using four repeated EAAAK sequences in chimeric gene has shown that it could lead to logically acceptable results [19, 22]. …”

Section: Discussionmentioning

confidence: 99%

“…The cells were trypsinized and spread on LB agar plates. After 18 h incubation, the numbers of colonies in test and control plates were counted and the number of neutralized bacteria by antibody was calculated [22, 23]. …”

Section: Methodsmentioning

confidence: 99%

Immunogenicity of a Fusion Protein Comprising Coli Surface Antigen 3 and Labile B Subunit of Enterotoxigenic Escherichia coli

Alerasol

Latif

Gargari

1996

Iranian Biomedical Journal (IBJ); ISSN 1028-852x

Self Cite

View full text Add to dashboard Cite

“…Isolation of E. coli from milk represent a serious public health hazard because some strains of E .coli may be belongs to enteropathogenic or toxigenic or both groups which causes sever gastrointestinal disturbance (Thomas et al, 2017). Enterotoxigenic E. coli (ETEC) is one of the most common bacteria responsible for diarrhea in different parts of the world (Bagheri et al, 2014). Like other gastrointestinal infectious diseases, they are caused by lack of sanitation and most often contamination transfers from contaminated food or water (Walker et al, 2007;Marchou, 2013).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and virulence determinants of Escherichia coli isolated from raw cows milk

Younis¹,

Awad²,

Ghabour³

2018

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Raw milk plays an important role in the survival and transport of pathogenic bacteria includingEscherichia coli strains. This study was performed to determine the existence of E. coli in raw milk intended for human consumption and its associated virulence determinants. In this context, a total of 232 milk samples were obtained from different dairy shops located at Mansoura city and its surrounding villages. Milk samples were subjected for bacteriological and serological examination of E. coli. Furthermore, E. coli strains were tested for its haemolytic activity on blood agar plates. The recovered E. coli strains were also screened by Polymerase chain reaction (PCR) for the presence of enterotoxins including heat -labile (LT), heat-stable (ST) toxins and haemolysin (hly) genes. The recovery rate of E. coli was 14.65% (34/232). Among the recovered E. coli strains, 12 different E. coli serotypes were identified namely, O26:H11 (n꞊6), O111:H2 (n꞊5), O128:H2 (n꞊5), O91:H21 (n꞊4), O124 (n꞊3), O127:H6 (n꞊3), O103:H21 (n꞊2), O153 (n꞊1), O113:H4 (n꞊2), O6 (n꞊1), O121:H7 (n꞊1) and O146 (n=1). Regarding PCR results, 31(91.19%) E. coli strains harbored STa and seven strains carried hly gene (20.59%) while non E. coli isoates harbord LT gene. Conclusively, raw milk can be considered as serious source of pathogenic E. coli, therefore, proper management practices and effective control measures are recommended to improve milk hygiene and sanitation.

show abstract

A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli

Abstract: The results indicate efficacy of the recombinant chimeric protein as an effective immunogen, which induces strong humoral response as well as protection against ETEC adherence and toxicity.

Cited by 12 publications

References 21 publications

Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates

Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates

Immunogenicity of a Fusion Protein Comprising Coli Surface Antigen 3 and Labile B Subunit of Enterotoxigenic Escherichia coli

Prevalence and virulence determinants of Escherichia coli isolated from raw cows milk

Contact Info

Product

Resources

About