Intestinal microsporidiosis in a reference center

Costa, Loredana; Marson, Fernando Augusto Lima; Teixeira, Angela Terezinha Lauand Sampaio; Sales, Célia Regina Mendes; Levy, Carlos Emílio

doi:10.1016/j.bjid.2013.07.004

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…Microsporidian spores were also identified in 1.3% of immunosuppressed patients in the city of Goiânia (Goiás, Brazil) (de Souza Jr & García-Zapata 2006), and in 36% of rheumatic disease patients undergoing anti-tumour necrosis factor therapy/anti-rheumatic drug treatment as compared to 4% of controls without diarrhoea (Aikawa et al 2011). In 2013, Costa et al (2013) described high positivity (40.3%) for microsporidia in patients who underwent transplantation surgery in the University Clinical Hospital, including bone marrow (75%), kidney (22%), and liver (3%) transplantation. These studies have shown that microsporidiosis is associated with chronic diarrhoea in immunocompromised patients and other immunosuppressed individuals in Brazil.…”

Section: Discussionmentioning

confidence: 99%

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Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Yamashiro

Fiúza

Teixeira

et al. 2017

Mem. Inst. Oswaldo Cruz

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BACKGROUND Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spores, but can also result from direct contact with spores through broken skin, eye lesions, and sexual transmission, depending on the microsporidian species. Although there are reports of E. bieneusi found in humans and animals in Brazil, there are no published studies of environmental samples examined by molecular methods.OBJECTIVES The purpose of this study was to verify the presence of E. bieneusi in raw sewage and treated effluent from a combined system by molecular methods.METHODS Raw sewage and treated effluent samples collected from a combined system were analysed for the presence of E. bieneusi using the internal transcriber spacer (ITS) region of E. bieneusi by nested polymerase chain reaction.FINDINGS The analysis revealed E. bieneusi presence and a novel genotype (EbRB) in one raw sewage sample and one treated effluent.MAIN CONCLUSIONS The presence of E. bieneusi in final effluent indicates that the combined system may not remove microsporidian spores. This study is the first report of E. bieneusi in environmental samples in Brazil.

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Section: Discussionmentioning

confidence: 99%

“…During January 2013 to June 2014, Costa et al (2013) verified several cases of microsporidiosis among transplant patients and HIV+ individuals attended by the University Clinical Hospital (University of Campinas, Campinas, Brazil) by microscopy techniques.…”

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confidence: 97%

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Yamashiro

Fiúza

Teixeira

et al. 2017

Mem. Inst. Oswaldo Cruz

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“…Isospora belli), Microsporidia (Enterocytozoon bieneusi and Encephalitozoon intestinalis), and Strongyloides stercoralis, although current parasites are also met (Entamoeba histolytica, Giardia intestinalis, Trichuris trichiura, Ascaris lumbricoides, and Ancylostoma duodenale) [2,5]. The resurgence of intestinal parasitic infections, previously considered being sporadic, no pathogenic or with transient pathogenic potential in immunecompetent individual, continues to attract more interest in HIV-positive patients because of their high frequency and their gravity [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Opportunistic and other intestinal parasites infections among HIVpositive patients in the era of combination antiretroviral therapy and preventive treatment in Ouagadougou, Burkina Faso

Yacouba

2017

J HIV Clin Sci Res

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Background: One of the particularities of human immunodefi ciency virus (HIV) infection in the tropics is its frequent association with parasitic diseases so frequent in this area. Aim: The aim of this study was to update the epidemiological and clinical profi le of intestinal parasites among HIV-positive patients in the era of combination antiretroviral therapy (ART) and preventive treatment in Ouagadougou, Burkina Faso. Materials and Methods: A cross-sectional study was conducted in a single health care system, Yalgado Ouédraogo University hospital, between January 2013 and December 2014. A total of 131 HIVinfected patients were included in the study. Blood and Stool samples were collected for CD4 counts and for intestinal parasitic examination using direct, formol-ether concentration, a concentration method using sodium chloride solution, modifi ed Ziehl-Neelsen and Weber modifi ed trichrome. Odds ratio was calculated to estimate the risk attributable to different factors with confi dence intervals. Chi-square (X 2) or Fisher's Exact Test statistical analysis was used to test level of signifi cance at p<0.05 using SPSS 20.0.0. Results: A prevalence of 73.3% of intestinal parasites was recorded. Most of the parasitic infections were protozoa (97.9%) with few helminths (2.1%). Cryptosporidium spp. (29.2%), was the most commonly found parasite followed by Entamoeba coli (23.9%) and Isospora belli (21.9%). There were 76 cases (78.5%) of single infection and 20 cases (21.5%) of mixed infection. Paradoxically, patients who use preventive treatment were more infected with opportunistic parasites (p= 0.05, OR=2.16, 95% CI= 1.06-4.40). In multivariate analysis, patients with diarrhea (OR=4.04, 95% CI=1.94-8.41), and illiterate (OR=3.70, 95% CI=1.68-8.14) had higher risk of opportunistic parasites. The diarrheic patients were 0.29 times more likely to be infected with opportunistic parasites. Conclusion: Despite the advent of combination ART and preventive treatment of opportunistic infections, intestinal parasites remain very prevalent and associated with diarrhea among patients with HIV in Ouagadougou.

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Intestinal microsporidiosis in a reference center

Cited by 2 publications

References 5 publications

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Opportunistic and other intestinal parasites infections among HIVpositive patients in the era of combination antiretroviral therapy and preventive treatment in Ouagadougou, Burkina Faso

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