Association between toll-like receptor2 Arg677Trp and 597T/C gene polymorphisms and pulmonary tuberculosis in Zahedan, Southeast Iran

Naderi, Mohammad; Hashemi, Mohammad; Hazire-Yazdi, Leylisadat; Taheri, Mohsen; Moazeni‐Roodi, Abdolkarim; Eskandari-Nasab, Ebrahim; Bahari, Gholamreza

doi:10.1016/j.bjid.2012.12.009

Cited by 31 publications

(17 citation statements)

References 35 publications

(38 reference statements)

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“…Moreover, in a study on an Iranian population from Zahedan, a significant difference was reported in TLR2 597T/C polymorphism genotype between patients with TB and healthy controls. However, no significant difference was reported between the groups regarding Arg677Trp (C2029T) polymorphism (30).…”

Section: Discussionmentioning

confidence: 79%

Arg677Trp and Arg753Gln Polymorphisms in TLR2 Genes Detected in Patients With Tuberculosis in Golestan Province, Iran

Davoodi

Ghaemi

Jamali

et al. 2018

Jundishapur J Microbiol

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Introduction: Toll-like receptors (TLRs), especially TLR2, play an important role in the immune responses of patients with tuberculosis (TB). The prevalence of TB is estimated 38.15 per 100,000 population in Golestan Province, Iran, which is higher than the rates reported in other provinces. Objectives: The current study aimed at detecting Arg677Trp and Arg753Gln polymorphisms in TLR2 genes detected in patients with TB in Golestan province. Methods: A total of 130 blood samples were collected from patients with sputum smear-positive TB, and 130 blood samples were collected from healthy individuals. Two polymorphisms of TLR2 gene, i e, Arg677Trp and Arg753Gln, were investigated via polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) and sequencing methods. Results: History of corticosteroid use was more frequently reported in patients, compared to the controls, while the frequency of Bacillus calmette-guerin (BCG) vaccination was more reported in controls. PCR-SSCP analysis of the TLR2 Arg677Trp region showed an almost identical pattern for patients and controls, while 2 SSCP patterns were observed in the amplicon, related to the Arg753Gln polymorphism. DNA sequencing for the Arg677Trp polymorphism showed a duplicated pseudogene, similar to the mutant sequence (C > T), as this polymorphism cannot be considered conclusive. The Arg753Gln polymorphism was not found in the samples, although nucleotide deletion (G) was detected at position 808 among the patients. Conclusions: The Arg753Gln polymorphism was not involved in susceptibility to TB in the current study population. The presence of pseudogenes in some genes such as TLR2 necessitates careful interpretation of such studies.

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Section: Discussionmentioning

confidence: 79%

Arg677Trp and Arg753Gln Polymorphisms in TLR2 Genes Detected in Patients With Tuberculosis in Golestan Province, Iran

Davoodi

Ghaemi

Jamali

et al. 2018

Jundishapur J Microbiol

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“…Moreover, molecular epidemiological study has shown that GT repeat variation at the microsatellite region of exon 2 of the TLR2 gene is significantly associated with an increased risk of PTB . However, molecular epidemiological study did not find any significant association of R753Q (rs5743708) and R677W (no rs number) polymorphisms of TLR2 gene with the risk of PTB in Indian and Iranian populations . Molecular studies have shown that 22 bp deletion at the promoter region of TLR2 gene is capable to alter the promoter activity .…”

Section: Discussionmentioning

confidence: 96%

Association of VDR gene polymorphisms and 22 bp deletions in the promoter region of TLR2Δ22 (‐196‐174) with increased risk of pulmonary tuberculosis: A case‐control study in tea garden communities of Assam

Devi

Mukherjee

Chelleng

et al. 2018

Clinical Laboratory Analysis

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“…This case-control study was performed on 188 PTB patients and 169 age-and gender-matched healthy individuals. The enrollment process and study design are described elsewhere (17)(18)(19)(20)(21)(22)(23). Briefly, the cases were chosen from PTB patients admitted to a University-Affiliated Hospital (Bou-Ali Hospital, Zahedan, Iran, referral center for TB) with no clinical symptoms or family history of TB.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the cases were chosen from PTB patients admitted to a University-Affiliated Hospital (Bou-Ali Hospital, Zahedan, Iran, referral center for TB) with no clinical symptoms or family history of TB. TB was diagnosed by clinical symptoms, posterior-anterior chest radiography, presence of acid-fast-bacilli on a sputum smear, and culturing MTB organisms from a specimen taken from the patient and response to therapy, as described previously (20)(21)(22)(23). The project was approved by the local Ethics Committee of the Zahedan University of Medical Sciences and informed consent was obtained from all subjects.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of interferon-induced transmembrane protein-3 (IFITM3) rs7478728 and rs3888188 polymorphisms and the risk of pulmonary tuberculosis

et al. 2016

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Abstract. The current study aimed to examine the possible association between the interferon-induced transmembrane protein-3 (IFITM3) gene polymorphisms and risk of pulmonary tuberculosis (PTB) in a sample population. This case-control study was conducted on 188 PTB patients and 169 healthy subjects. The rs7478728 and rs3888188 variants of IFITM3 were genotyped using polymerase chain reaction-restriction fragment length polymorphism. The findings showed no significant association between rs7478728 polymorphism and risk of PTB. Regarding rs3888188 polymorphism, the TG genotype as well as G allele significantly increased the risk of PTB [odds ratio (OR)=2.48, 95% confidence interval (CI): 1.42-4.53; P=0.002, and OR=2.26, 95% CI: 1.33-3.86; P= 0.003, respectively]. In conclusion, the findings revealed that rs3888188 polymorphism increased the risk of PTB in a sample of Iranian population. Additional investigation with larger sample sizes and different ethnicities are needed to verify our findings. IntroductionTuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) infection, is a public health problem globally (1,2). According to the WHO report on the worldwide control of TB, approximately 8.6 million new cases occurred in 2012 (3). Although almost 33% of the population is infected with TB, only 5-10% of infected cases develop active TB (3), which suggests a major role of genetic factors in host immunity.Interferon-γ (IFNγ) is produced and released by host cells in response to the presence of numerous pathogens (4). It plays a key role in macrophage activation during MTB infection (5).Individuals defective in the genes for IFNγ or IFNγ receptor (IFNγR) have been indicated to be susceptible for mycobacterial infections including MTB (6). Previously, we showed an association between IFNγ and IFNγR variants and risk of pulmonary TB (PTB) (7,8).Interferon-induced transmembrane protein-3 (IFITM3) is a double transmembrane protein that can be upregulated by IFNs and participates in INF-triggered processes, such as homotypic cell adhesion, anti-proliferative activities in tumor pathogenesis, and the innate immune response to virus infections (9-13). The IFITM3 gene is mapped to an IFITM gene cluster on chromosome 11p15.5 (14). In a genome wide scan, Stein et al (15) identified that one of the TB-linked loci was located in this chromosome region. To the best of our knowledge, there is only one report regarding the impact of IFITM gene polymorphisms on the risk of TB (16). Therefore, the present study aimed to examine the possible associations between polymorphisms of IFITM3 gene and susceptibility to PTB in a sample of Iranian population. Materials and methodsPatients. This case-control study was performed on 188 PTB patients and 169 age-and gender-matched healthy individuals. The enrollment process and study design are described elsewhere (17-23). Briefly, the cases were chosen from PTB patients admitted to a University-Affiliated Hospital (Bou-Ali Hospital, Zahedan, Iran, referral center for TB) with no clinical sy...

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Association between toll-like receptor2 Arg677Trp and 597T/C gene polymorphisms and pulmonary tuberculosis in Zahedan, Southeast Iran

Abstract: Our data suggest that 597T/C polymorphism, but not Arg677Trp polymorphism, of the TLR-2 gene is a risk factor for susceptibility to PTB in a sample of Iranian population.

Cited by 31 publications

References 35 publications

Arg677Trp and Arg753Gln Polymorphisms in TLR2 Genes Detected in Patients With Tuberculosis in Golestan Province, Iran

Arg677Trp and Arg753Gln Polymorphisms in TLR2 Genes Detected in Patients With Tuberculosis in Golestan Province, Iran

Association of VDR gene polymorphisms and 22 bp deletions in the promoter region of TLR2Δ22 (‐196‐174) with increased risk of pulmonary tuberculosis: A case‐control study in tea garden communities of Assam

Evaluation of interferon-induced transmembrane protein-3 (IFITM3) rs7478728 and rs3888188 polymorphisms and the risk of pulmonary tuberculosis

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