Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil

Abstract

The zoonotic potential to cause human and/or animal infections among multidrug-resistant extraintestinal pathogenic Escherichia coli from avian origin was investigated. Twenty-seven extraintestinal pathogenic E. coli isolates containing the increased survival gene (iss) were obtained from the livers of healthy and diseased poultry carcasses at two slaughterhouses in Salvador, northeastern Brazil. The antimicrobial resistance-susceptibility profiles were conducted with antibiotics of avian and/or human use by the standardized disc-diffusion method. Antimicrobial resistance was higher for levofloxacin (51.8%), amoxicillin/clavulanic acid (70.4%), ampicillin (81.5%), cefalotin (88.8%), tetracycline (100%) and streptomycin (100%). The minimum inhibitory concentrations above the resistance breakpoints of doxycycline, neomycin, oxytetracycline and enrofloxacin reached, respectively, 88.0%, 100%, 75% and 91.7% of the isolates. Strains with high and low antimicrobial resistance were i.p. administered to Swiss mice, and histopathological examination was carried out seven days after infection. Resistance to goat and human serum complement was also evaluated. The results show that Swiss mice challenged with strain 2B (resistant to 11 antimicrobials) provoked a severe degeneration of hepatocytes besides lymphocytic infiltration in the liver, whereas the spleen showed areas of degeneration of the white and red pulp. Conversely, the spleen and liver of mice challenged with strain 4A (resistant to two antimicrobials) were morphologically preserved. In addition, complement resistance to goat and human serum was high for strain 2B and low for strain 4A. Our data show that multidrug resistance and pathogenesis can be correlated in extraintestinal pathogenic E. coli strains obtained from apparently healthy poultry carcasses, increasing the risk for human public healthy.

Isolation of Salmonella species and some other gram negative bacteria from rats cohabitating with poultry in Ibadan, Oyo State, Nigeria

“…Serratia liquefaciens had been implicated in the contamination of chicken carcasses (Lahellec et al, 1975) and it is also occasionally isolated from clinical specimens, although its pathogenic role has not been established (Grimont and Grimont, 1984). The presence of Proteus mirabilis in poultry meat had also been documented (Kim et al, 2005, Wong et al, 2013, and the presence of Proteus species in chicken droppings had been acknowledged as one of the route of its transmission as a zoonotic pathogens to vulnerable workers while handing infected chicken directly (Tonkic et al, 2010) or through fecal-contaminated poultry products as other Enteric bacteria (Lima-Filho et al, 2013). A more current report also associated poultry-borne Proteus species with zoonotic urinary tract infection (Armbruster et al, 2014).It was also associated with rheumatoid arthritis that is common in developing countries (Ebringer and Rashid, 2014).…”

Phylogenetic characterization of serum plus antibiotic-resistant extraintestinal Escherichia coli obtained from the liver of poultry carcasses in Pernambuco

“…In a previous study, we have shown that multidrug-resistant E. coli harboring the iss gene obtained from poultry carcasses may resist the human serum and cause pathogenesis in the murine model (Lima-Filho et al 2013). In this study, avian extraintestinal Escherichia coli obtained from the liver of poultry carcasses approved for human consumption in the State of Pernambuco-Brazil were tested for antibiotic plus serum-resistance.…”

Multidrug Resistant-Proteus Mirabilis Isolated from Chicken Droppings in Commercial Poultry Farms: Bio-security Concern and Emerging Public Health Threat in Bangladesh

“…Several authors reported presence of P. mirabilis in poultry meat [8,9]; none of similar type of data had been reported from in Bangladesh. Thus the aforementioned facts prompted us to investigate the presence of P. mirabilis in chicken droppings of Bangladeshi poultry that might be transmitting this zoonotic pathogen [10] to vulnerable workers while handing infected chicken directly or through fecal-contaminated poultry products as similarly enterobacteriaceae have been reported to transmit [11,12].…”