Label-free detection of ATP release from living astrocytes with high temporal resolution using carbon nanotube network

Huang, Yongmei; Sudibya, Herry Gunadi; Fu, Dongliang; Xue, Rongjian; Dong, Xiaochen; Li, Lain‐Jong; Chen, Peng

doi:10.1016/j.bios.2008.12.006

Cited by 59 publications

(37 citation statements)

References 26 publications

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“…The dynamic detection of the release of biomolecules from living cells in real time is important both in fundamental studies and in the evaluation of drugs for the treatment of secretion-related diseases. To this goal, Huang et al 55 utilized a SWNT network to directly interface with living neuroglial astrocytes and, without labels, detect the triggered release of ATP from these cells. This detection scheme showed high temporal resolution.…”

Section: Swnt Fet-based Biosensors Swnt àDynamic Detection In Living mentioning

confidence: 99%

Carbon nanomaterials field-effect-transistor-based biosensors

2012

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Carbon nanomaterials field-effect transistor (FET)-based electrical biosensors provide significant advantages over the current gold standards, holding great potential for realizing direct, label-free, real-time electrical detection of biomolecules in a multiplexed manner with ultrahigh sensitivity and excellent selectivity. The feasibility of integrating them with current complementary metal oxide semiconductor platform and a fluid handling module using standard microfabrication technology opens up new opportunities for the development of low-cost, low-noise, portable electrical biosensors for use in practical future devices. In this article, we review recent progress in the rapidly developing area of biomolecular interaction detection using FET-based biosensors based on the carbon nanomaterials single-walled carbon nanotubes (SWNTs) and graphene. Detection scenarios include DNA-DNA hybridization, DNA-protein interaction, protein function and cellular activity. In particular, we will highlight an amazing property of SWNT-or graphene-FETs in biosensing: their ability to detect biomolecules at the single-molecule level or at the single-cell level. This is due to the size comparability and the surface compatibility of the carbon nanomaterials with biological molecules. We also summarize some current challenges the scientific community is facing, including device-to-device heterogeneity and the lack of system integration for uniform device array mass production. Keywords: biosensor; carbon nanotube; field-effect transistor; graphene A biosensor is an analytical device that incorporates a biological recognition element in direct spatial contact with a transduction element. This integration ensures the rapid and convenient conversion of the biological events to detectable signals. 1 With the discovery of rich nanomaterials and the development of exquisite nanofabrication tools, such as electron beam lithography, focused ion beam and nanoimprint lithography, new avenues have been opened up in the field of biosensors in the last two decades. 2,3 In particular, researchers around the world have been tailor-making a multitude of nanomaterials-based electrical biosensors and developing new strategies to apply them in ultrasensitive biosensing. Examples of such nanomaterials include carbon nanotubes, 4-13 nanowires, 11,14-21 nanoparticles, 6,22-25 nanopores, 26,27 nanoclusters 28 and graphene. 5,[29][30][31][32] Compared with conventional optical, biochemical and biophysical methods, nanomaterial-based electronic biosensing offers significant advantages, such as high sensitivity and new sensing mechanisms, high spatial resolution for localized detection, facile integration with standard wafer-scale semiconductor processing and label-free, real-time detection in a nondestructive manner.Among diverse electrical biosensing architectures, devices based on field-effect transistors (FETs) have attracted great attention because they are an ideal biosensor that can directly translate the interactions between target biological mole...

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Section: Swnt Fet-based Biosensors Swnt àDynamic Detection In Living mentioning

confidence: 99%

Carbon nanomaterials field-effect-transistor-based biosensors

2012

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“…Glycosylated SWNTs-net can directly interface with living cells by supporting their adhesion and growth for monitoring dynamic biomolecular release from these cells [77]. Using a CNT network, the label-free detection of ATP release from living astrocytes was developed with high temporal resolution [78].…”

Section: Cellsmentioning

confidence: 99%

Electrochemical Biosensing Based on Carbon Nanotubes

Zhang

Wang

2011

NanoBiosensing

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“…Moreover, since the current flows along the nanotube surface, the electrical conductance of the nanotube is highly sensitive to chemical changes on its sidewalls [10]. In these devices the binding of a target molecule to the carbon nanotube surface is detected by a change in electrical signal, such as through a change in conductance [11].…”

Section: Introductionmentioning

confidence: 99%

“…These types of biosensors have been used to detect E. coli [11], or glucose molecules [12,13]. Moreover, they can also be used to study dynamic processes, such as the release of ATP from living cells [10]. Sotiropoulou and Chaniotakis [12] used an array of multi-walled carbon nanotubes grown on a platinum substrate.…”

Section: Introductionmentioning

confidence: 99%

Computational Design of a Carbon Nanotube Fluorofullerene Biosensor

Hilder

Pace

Chung

2012

Sensors

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Carbon nanotubes offer exciting opportunities for devising highly-sensitive detectors of specific molecules in biology and the environment. Detection limits as low as 10−11 M have already been achieved using nanotube-based sensors. We propose the design of a biosensor comprised of functionalized carbon nanotube pores embedded in a silicon-nitride or other membrane, fluorofullerene-Fragment antigen-binding (Fab fragment) conjugates, and polymer beads with complementary Fab fragments. We show by using molecular and stochastic dynamics that conduction through the (9, 9) exohydrogenated carbon nanotubes is 20 times larger than through the Ion Channel Switch ICS™ biosensor, and fluorofullerenes block the nanotube entrance with a dissociation constant as low as 37 pM. Under normal operating conditions and in the absence of analyte, fluorofullerenes block the nanotube pores and the polymer beads float around in the reservoir. When analyte is injected into the reservoir the Fab fragments attached to the fluorofullerene and polymer bead crosslink to the analyte. The drag of the much larger polymer bead then acts to pull the fluorofullerene from the nanotube entrance, thereby allowing the flow of monovalent cations across the membrane. Assuming a tight seal is formed between the two reservoirs, such a biosensor would be able to detect one channel opening and thus one molecule of analyte making it a highly sensitive detection design.

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Label-free detection of ATP release from living astrocytes with high temporal resolution using carbon nanotube network

Cited by 59 publications

References 26 publications

Carbon nanomaterials field-effect-transistor-based biosensors

Carbon nanomaterials field-effect-transistor-based biosensors

Electrochemical Biosensing Based on Carbon Nanotubes

Computational Design of a Carbon Nanotube Fluorofullerene Biosensor

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