Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae

Effects of titanium dioxide nanoparticles on lead bioconcentration and toxicity on thyroid endocrine system and neuronal development in zebrafish larvae

“…Thus, the alterations in the TH levels (decrease or increase) may produce several developmental effects during larval development, especially in the CNS (Chen et al, 2012b;Wang et al, 2014). In the present study, the total T 4 and T 3 concentrations decreased in all the Pb treatment groups.…”

“…The extraction, purification and quantification of total RNA as well as the synthesis of first-strand cDNA were performed as previously described in Chen et al (2012b). The DNA contaminants were removed by RNase-free DNase I (Promega, Madison, WI, USA), and the RNA concentrations were immediately quantified by UV spectrophotometry (M2; Molecular Devices, CA, USA) at 260 nm.…”

“…Adult wild-type zebrafish (D. rerio, AB strain) maintenance and embryo exposure were performed as previously described by Chen et al (2012b). Briefly, embryos that had developed normally and reached the blastula stage (2 h post-fertilization, hpf) were selected.…”

PBDE flame retardants

“…106 In addition, an increase in dio2 mRNA transcripts have been measured in the larvae of Chinese rare minnows exposed to BDE-209 with a decrease in dio2 transcripts measured in the brains of adults minnows. 155 Recently published data of ours has shown TH regulation and signaling in juvenile and adult fathead minnows to be disrupted by low dose exposures to BDE-209.…”

“…As many as 10 different UDPGT isoforms have been identified in zebrafish, with nucleotide similarities to some mammalian UGT1 and UGT2 gene families. 105 Two studies have shown a decrease in the relative mRNA abundances of genes encoding UDPGT1ab in zebrafish larvae exposed to BDE-209 106 and UGT1 in juvenile Atlantic cod (G. morhua) exposed to BDE-47. 82 This decline may be a response to reduced TH levels as UDPGTs are involved in the metabolism of THs.…”