In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation

Saxena, Sanjay

doi:10.1007/bf00232266

Cited by 66 publications

(55 citation statements)

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“…Arya and Sharma, (1998);Anand et al, (2013) and Nayak et al, (2010) investigated that the cytokinins (BAP and Kn) were effective in direct shoot induction of the axillary buds of B. bambos as well as Devi andSharma, (2009) in Arundinaria callosa, andChaudhary et al, (2004) in D. strictus. BAP has also been found to be essential for direct shoot induction in bamboo (Nadgir et al, 1984;Banik, 1987 andSaxena, 1990). In the present study, directly induced shoots were excised from nodal segments after 4 weeks of growth on initiation medium and cultured on MS gelled medium supplemented with different concentrations of BAP alone or in combination with TDZ, Kn and NAA.…”

Section: Resultsmentioning

confidence: 81%

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Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog's (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.

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Section: Resultsmentioning

confidence: 81%

“…There are some reports that addition of auxins (IAA or NAA) has either no effect on multiplication or decreased the multiplication of bamboo (Saxena, 1990;Das and Rout, 1991;Prutpongse and Gavinlertvatana, 1992).…”

Section: Resultsmentioning

confidence: 99%

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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“…Hardened and acclimatized in vitro plantlets of D. falcatum in pots in polyhouse (Figure 1(f), Figure 1(g) [39]. Use of vermiculite or soilrite like inert substance for hardening has been reported by many workers in bamboos [19] [37]. Vermiculite is an inert material and absorbs large quantity of water.…”

Section: Formation Of Roots and Acclimatizationmentioning

confidence: 99%

<i>In Vitro</i> Micropropagation of Himalayan Weeping Bamboo, <i>Drepanostachyum falcatum</i>

Saini¹,

Arya²,

Arya³

et al. 2016

AJPS

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“…Tissue culture is one essential technique to micropropagate regenerated plant tissues and it is also a pre-requisite for genetic improvement through the use of different transformation strategies. At present, reports dealing with bamboo tissue culture are described mainly for the genus Bambusa (Kalia et al 2004;Lin et al 2003Lin et al , 2004Saxena 1990) and Dendrocalamus (Ramanayake et al 2001;Saxena and Dhawan 1999;Singh et al 2003;Sood et al 2002). Little information is available for the genus Phyllostachys.…”

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confidence: 99%

Callus and cell suspension culture of bamboo plant, Phyllostachys nigra

Ogita

2005

Plant Biotechnology

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A modified 1/2MS medium supplemented with 3 mM 2,4-D was effective for callus induction from bamboo shoots of Phyllostachys nigra Munro var. Henonis. In the first phase (phase 1), some parts of the explants enlarged and gave rise to whitish-yellow calli after 2-3 weeks of culture. During maintenance subcultures, almost all explants and the initially formed calli turned brown and these calli gradually lost their proliferation capacity (phase 2). Removal of the necrotic potions of explants, and frequent subcultures at phase 2 was essential. Secondary proliferated calli were subsequently produced on the surface of brown tissues (phase 3). These calli could be maintained on both solid and liquid media. The liquid suspension cells had a blue to pale blue autofluorescence in the cell walls. These cells fluoresced strongly when stained with Calcofluor White M2R and Aniline Blue, indicating the presence of callose (b-1,3-glucan) in a cellulosic wall. Endogenous free amino acids analyses indicated that glutamine, g-aminobutyric acid, and alanine were the major amino acids in callus tissues whereas asparagine and tyrosine were abundant in the regenerated bamboo shoots.Key words: Bamboo, callus, Phyllostachys nigra, suspension culture. Original Paper Copyright © 2005 The Japanese Society for Plant Cell and Molecular BiologyAbbreviations: BA, 6-benzyladenine; 2,4-D, 2,4-dichlorophenoxyacetic acid; MS medium, Murashige and Skoog medium; Picloram, 4-amino-3,5,6-trichloropyridine-2-carboxylic acid. Materials and methods Plant materialsBamboo shoots of P. nigra were collected in mid-May 2003 and from early to mid-June 2004 at Takayama, Nara, Japan. Young bamboo shoots 20-30 cm in length were selected as plant materials. Culm-sheaths were removed, and then washed in water with several drops of a detergent for 30 min. After soaking, the shoots were surface sterilized first with 70% ethyl alcohol for 20 min followed by a 2% NaClO with several drops of Tween 20 for 60 min. After sterilization, they were rinsed 3 times with sterile distilled water, dried on sterile paper, and used as explants. Induction and maintenance of bamboo calliA modified half strength Murashige and Skoog (m1/2MS) medium (Murashige and Skoog 1962) was used as the basal medium. The concentrations of inorganic elements were reduced to half of the original and 30 g l Ϫ1 sucrose was added. Plant growth regulators 2,4-D, Picloram, and BA were added to m1/2MS medium at 0, 1, 3, 10, and 30 mM, respectively. The pH of the media was adjusted to 5.7 and 3 g l Ϫ1 gellan gum was added before autoclaving. The complete media were autoclaved for 20 min at 120°C and a 20 ml aliquot of each medium was poured into a 90ϫ15 mm petri dish. Inner portions of the sterile bamboo shoots (ca. 1-3 cm 2 ) were excised and 2-4 pieces of the excised tissues were cultured on each dish. Maintenance subculture was carried out on m1/2MS media of the same compositions of the induction media at 3-to 4-week intervals. All the dishes with explants were incubated in the dark at 26°C. Initi...

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In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation

Cited by 66 publications

References 16 publications

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

<i>In Vitro</i> Micropropagation of Himalayan Weeping Bamboo, <i>Drepanostachyum falcatum</i>

Callus and cell suspension culture of bamboo plant, Phyllostachys nigra

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In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation

Cited by 66 publications

References 16 publications

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

&lt;i&gt;In Vitro&lt;/i&gt; Micropropagation of Himalayan Weeping Bamboo, &lt;i&gt;Drepanostachyum falcatum&lt;/i&gt;

Callus and cell suspension culture of bamboo plant, Phyllostachys nigra

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<i>In Vitro</i> Micropropagation of Himalayan Weeping Bamboo, <i>Drepanostachyum falcatum</i>