A Bioorthogonal Approach for the Preparation of a Titanium‐Binding Insulin‐like Growth‐Factor‐1 Derivative by Using Tyrosinase

Zhang, Chen; Miyatake, Hideyuki; Wang, Yu; Inaba, Takehiko; Wang, Yi; Zhang, Peibiao; Ito, Yoshihiro

doi:10.1002/anie.201603155

Cited by 27 publications

(20 citation statements)

References 42 publications

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“…We anticipated that the immobilized bFGF should be more active to boost growth of NIH‐3T3 cells due to its expanded duration of effect. As highlighted in majority of recent studies, the immobilized growth factors have been confirmed to be positive in cell functions and tissue regeneration both in vitro and vivo environments . However, only a few researches explored the stimulation of cells by growth factors which could be immobilized on surfaces of biomaterials at certain densities of growth factors.…”

Section: Resultsmentioning

confidence: 99%

“…The method of adsorption/physisorption governed by hydrophobic, hydration and electrostatic force is greatly used for non‐site‐specific approach of growth factors to materials . On the other hand, attachment of growth factors to biomaterials (eg, polymers, metals, ceramics and composites) is enhanced by surface modification, covalent bonding, encapsulating, and biomaterial‐binding growth factors . Recently, a method to capture endogenous growth factors onto biomaterials by growth‐factor‐binding peptides was employed to overcome the supraphysiological doses for exogenous administration of growth factors .…”

Section: Introductionmentioning

confidence: 99%

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Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Zhou

Chen

et al. 2018

Biopolymers

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The recombinant basic fibroblast growth factor (bFGF) containing collagen-binding domain (CBD) has been found to be a potential therapeutic factor in tissue regeneration. However, its binding efficiency and quantification remain uncertain. In this research, massive recombinant bFGFs with good bioactivity for enhancing the proliferation of NIH-3T3 cells were achieved. An ELISA-based quantitative method was set up to investigate the binding efficiency of CBD-bFGFs on collagen films. It indicated that the CBDs significantly increased the collagen-binding ability of bFGF (P < .05), with the optimum binding condition first determined to be in the pH range of 7.5-9.5 (P < .05). Then, the relevant equations to calculate the binding density of bFGF, C-bFGF, and V-bFGF were acquired. Analysis confirmed that the bioactivity of immobilized bFGFs was well correlated with the density of growth factor on collagen films. Based on this research, the density of growth factor is a logical and applicable dosage unit for quantification of binding efficiency of growth factors, rather than traditional concentration of soluble growth factors in tissue engineering applications.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Zhou

Chen

et al. 2018

Biopolymers

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“…The PLGA and pDA/PLGA fibers were respectively soaked in 3 mL PBS solution (pH 7.2). 100 μL supernatant was collected as sample at each time point and detected the absorbance at 450 nm using an auto microplate reader (Tecan M200, Switzerland) as reported [23]. The quantitative absorption and release tests based on ELISA method were conducted as follow.…”

Section: Pda-assisted Immobilization Of Pdgf-bb Onto Fibersmentioning

confidence: 99%

Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion

et al. 2020

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Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its efficacy in vivo and vitro. To evaluate the wound healing effects of dorsal skin in SD rats with polydopamine-assisted immobilized PDGF-BB on PLGA nanofibrous substrate. First, the effects of pDA-coating and PDGF-BB immobilization on the morphology, compositions, and hydrophilicity of substrates were evaluated in details. Second, the wound healing effect of pDA/PLGA/PDGF-BB substrate was assessed in the dorsal skin of SD rats. Last, the cytokine analysis by ELISA method was employed to evaluate the advantages of pDA/PLGA/PDGF-BB substrate on anti-inflammatory, angiogenesis, and cellular proliferation. This method significantly improved the immobilization amount and stability of PDGF-BB on the substrate (p<0.01), further improved the hydrophilicity of substrates (p<0.05). Furthermore, the wound closure process was much more accelerated in the pDA/ PLGA/PDGF-BB group (p<0.05). H&E and CD31 staining informed that the wound treated by pDA/PLGA/PDGF-BB substrate showed a high degree of regeneration and angiogenesis. The cytokine analysis showed that pDA significantly reduced the high level of inflammatory cytokines such as TNF-α (p<0.05). And the immobilized PDGF-BB significantly elevated the level of TGF-β and VEGF (p<0.05). The pDA/PLGA/PDGF-BB substrate showed great therapeutic effect on wound healing compared with other control groups via regulating anti-inflammatory, angiogenesis, and cellular proliferation. Absolutely, this report offered an available novel method for skin regeneration.

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“…Various kinds of growth factors have been applied with special binding technology to enhance their binding capability on the material surface and obtain good tissue repair effect. Ito, Yoshihiro et al introduced 3,4-dihydroxyphenylalanine (DOPA) molecule at the end of insulin-like growth factor 1 (IGF1); the adhesion efficiency of DOPA-IGF1 on the Ti surface was enhanced due to the adhesion capability of DOPA [ 20 ]. To find a specific binding peptide with HA, Matthew L. Becker et al used phage display technology to identify a peptide sequence that exhibits high specificity binding to HA [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional printed hydroxyapatite bone tissue engineering scaffold with antibacterial and osteogenic ability

Liu

et al. 2021

J Biol Eng

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The development of an effective scaffold for bone defect repair is an urgent clinical need. However, it is challenging to design a scaffold with efficient osteoinduction and antimicrobial activity for regeneration of bone defect. In this study, we successfully prepared a hydroxyapatite (HA) porous scaffold with a surface-specific binding of peptides during osteoinduction and antimicrobial activity using a three-dimensional (3D) printing technology. The HA binding domain (HABD) was introduced to the C-terminal of bone morphogenetic protein 2 mimetic peptide (BMP2-MP) and antimicrobial peptide of PSI10. The binding capability results showed that BMP2-MP and PSI10-containing HABD were firmly bound to the surface of HA scaffolds. After BMP2-MP and PSI10 were bound to the scaffold surface, no negative effect was observed on cell proliferation and adhesion. The gene expression and protein translation levels of type I collagen (COL-I), osteocalcin (OCN) and Runx2 have been significantly improved in the BMP2-MP/HABP group. The level of alkaline phosphatase significantly increased in the BMP2-MP/HABP group. The inhibition zone test against Staphylococcus aureus and Escherichia coli BL21 prove that the PSI10/HABP@HA scaffold has strong antibacterial ability than another group. These findings suggest that 3D-printed HA scaffolds with efficient osteoinduction and antimicrobial activity represent a promising biomaterial for bone defect reconstruction.

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A Bioorthogonal Approach for the Preparation of a Titanium‐Binding Insulin‐like Growth‐Factor‐1 Derivative by Using Tyrosinase

Cited by 27 publications

References 42 publications

Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion

Three-dimensional printed hydroxyapatite bone tissue engineering scaffold with antibacterial and osteogenic ability

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