“…The commonly used methods for the dewaxing of cotton for analytical or research purposes utilize periods of extraction varying from 4 hours [1,2,4] to two successive periods of 24 hours each [27] and use a great variety of solvents, techniques, and temperatures which are frequently inadequately specified. Benzene [13,22], chloroform [6,12], carbon tetrachloride [20], ethyl alcohol [9], isopropyl alcohol [17], alcohol-benzene mixture [15], trichloroethylene [10], and ether and other solvents [5] have been used or recommended for extraction of the cotton wax.…”