1-Nitro-2-phenylethane, the main constituent of the essential oil of Aniba canelilla, elicits a vago-vagal bradycardiac and depressor reflex in normotensive rats

Siqueira, Rodrigo José Bezerra de; Macedo, Francisco Igor B.; Interaminense, Leylliane de Fátima Leal; Duarte, Glória Pinto; Magalhães, Pedro Jorge Caldas; Brito, Teresinha Silva de; Silva, Joyce Kelly R. da; Maia, José Guilherme S.; Sousa, Pergentino José da Cunha; Leal-Cardoso, José Henrique; Lahlou, Saad

doi:10.1016/j.ejphar.2010.03.060

Cited by 35 publications

(34 citation statements)

References 26 publications

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“…The effects of NP described in this study in SHRs were similar to those recently reported in normotensive rats by de Siqueira et al [24]. In brief, NP-induced cardiovascular responses also characterized by two phases (phases 1 and 2) mediated through a similar mechanism as that reported herein in SHRs, i.e.…”

Section: Discussionsupporting

confidence: 91%

“…In brief, NP‐induced cardiovascular responses also characterized by two phases (phases 1 and 2) mediated through a similar mechanism as that reported herein in SHRs, i.e. stimulation of vagal pulmonary C‐fiber afferents with no participation of both vanilloid TPRV 1 and 5‐HT 3 receptors for NP‐induced phase 1 vago‐vagal reflex and peripheral vasodilatation for NP‐induced phase 2 hypotension response [24]. Assuming that a similar phenomenon would probably be observed in Wistar Kyoto rats, control experiments in these animals were not considered as necessary in this study.…”

Section: Discussionmentioning

confidence: 75%

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Cardiovascular effects of 1-nitro-2-phenylethane, the main constituent of the essential oil of Aniba canelilla, in spontaneously hypertensive rats

Interaminense

Siqueira

Xavier

et al. 2010

Fundamental &Amp; Clinical Pharmacology

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This study investigated the cardiovascular responses to the essential oil of Aniba canelilla (EOAC) and its main constituent 1-nitro-2-phenylethane (NP) in spontaneously hypertensive rats (SHRs). In anesthetized SHRs, intravenous (i.v.) bolus injections of EOAC (1-20 mg/kg) or NP (1-10 mg/kg) elicited dose-dependent hypotensive and bradycardiac effects, which were characterized in two periods (phases 1 and 2). The first rapid component (phase 1) evoked by EOAC and NP both at 10 mg/kg was absent after left ventricle injection, fully abolished by bilateral vagotomy and perineural treatment of both cervical vagus nerves with capsaicin (250 μg/mL) while remained unaltered by i.v. pretreatment with capsazepine (1 mg/kg) or ondansetron (30 μg/kg). In conscious SHRs, NP (5 and 10 mg/kg, i.v.) evoked rapid hypotensive and bradycardiac effects (phase 1) that were fully abolished by methylatropine (1 mg/kg, i.v.) pretreatment. In rat endothelium-containing mesenteric preparations, increasing concentrations (0.1-1000 μg/mL) of EOAC and NP relaxed the phenylephrine-induced contraction in a concentration-dependent manner. It is concluded that NP induces a vago-vagal bradycardiac and depressor reflex (phase 1) that apparently results from the stimulation of vagal pulmonary rather than cardiac C-fiber afferents. This effect does not appear to involve activation of either vanilloid TPRV(1) or 5-HT(3) receptors located on vagal sensory nerves. The phase 2 hypotensive response to i.v. NP seems to result, at least in part, from its direct vasodilatory effect on the peripheral smooth muscle. All in vivo and in vitro effects of EOAC are mostly attributed to the actions of its main constituent NP.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 75%

Cardiovascular effects of 1-nitro-2-phenylethane, the main constituent of the essential oil of Aniba canelilla, in spontaneously hypertensive rats

Interaminense

Siqueira

Xavier

et al. 2010

Fundamental &Amp; Clinical Pharmacology

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“…Perivagal capsaicin treatment has been widely used as an intervention to study the role of vagal lung C fibers in eliciting reflex changes in breathing patterns (9,28,33,43,49), in reflex bronchoconstriction (34,48), and in airway hypersensitivity (17,18) using whole animal models. Capsaicin, a chemical extracted from hot peppers, is an agonist targeting TRPV1 receptors (29).…”

Section: Perivagal Antagonist Treatment In Rats Selectively Blocks Thmentioning

confidence: 99%

“…Capsaicin, a chemical extracted from hot peppers, is an agonist targeting TRPV1 receptors (29). When capsaicin is applied perineurally to the vagus nerves, the treatment blocks the neural conduction of vagal C fibers (40,52) and C-fiber-mediated airway responses (9,17,18,33,34,43,48,49). However, perivagal capsaicin treatment cannot be used to delineate the specific types of pharmacological receptors involved, because it nonselectively prevents airway responses resulting from activation of a number of different types of pharmacological receptors located at vagal lung C-fiber terminals (33,43,49).…”

Section: Perivagal Antagonist Treatment In Rats Selectively Blocks Thmentioning

confidence: 99%

Perivagal antagonist treatment in rats selectively blocks the reflex and afferent responses of vagal lung C fibers to intravenous agonists

Lin¹,

Lin

Lai

et al. 2013

Journal of Applied Physiology

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The terminals of vagal lung C fibers (VLCFs) express various types of pharmacological receptors that are important to the elicitation of airway reflexes and the development of airway hypersensitivity. We investigated the blockade of the reflex and afferent responses of VLCFs to intravenous injections of agonists using perivagal treatment with antagonists (PAT) targeting the transient receptor potential vanilloid 1, P2X, and 5-HT(3) receptors in anesthetized rats. Blockading these responses via perivagal capsaicin treatment (PCT), which blocks the neural conduction of C fibers, was also studied. We used capsaicin, α,β-methylene-ATP, and phenylbiguanide as the agonists, and capsazepine, iso-pyridoxalphosphate-6-azophenyl-2',5'-disulfonate, and tropisetron as the antagonists of transient receptor potential vanilloid 1, P2X, and 5-HT(3) receptors, respectively. We found that each of the PATs abolished the VLCF-mediated reflex apnea evoked by the corresponding agonist, while having no effect on the response to other agonists. Perivagal vehicle treatment failed to produce any such blockade. These blockades had partially recovered at 3 h after removal of the PATs. In contrast, PCT abolished the reflex apneic response to all three agonists. Both PATs and PCT did not affect the myelinated afferent-mediated apneic response to lung inflation. Consistently, our electrophysiological studies revealed that each of the PATs prevented the VLCF responses to the corresponding agonist, but not to any other agonist. PCT inevitably prevented the VLCF responses to all three agonists. Thus these PATs selectively blocked the stimulatory action of corresponding agonists on the VLCF terminals via mechanisms that are distinct from those of PCT. PAT may become a novel intervention for studying the pharmacological modulation of VLCFs.

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“…A short tracheal cannula was inserted via a tracheotomy, through which rats breathed spontaneously in the supine position. Baseline mean arterial pressure (MAP) and HR values were recorded on a Gilson model 5/6H (medical Electronics Inc., Middletown, WI, USA), as previously described (de Siqueira et al ., , ).…”

Section: Methodsmentioning

confidence: 99%

Linalool‐rich Rosewood Oil Induces Vago‐vagal Bradycardic and Depressor Reflex in Rats

Siqueira

Rodrigues

Silva

et al. 2013

Phytotherapy Research

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Cardiovascular effects of the linalool-rich essential oil of Aniba rosaeodora (here named as EOAR) in normotensive rats were investigated. In anesthetized rats, intravenous (i.v.) injection of EOAR induced dose-dependent biphasic hypotension and bradycardia. Emphasis was given to the first phase (phase 1) of the cardiovascular effects, which is rapid (onset time of 1-3 s) and not observed in animals submitted to bilateral vagotomy or selective blockade of neural conduction of vagal C-fibre afferents by perineural treatment with capsaicin. Phase 1 was also absent when EOAR was directly injected into the left ventricle injection, but it was unaltered by i.v. pretreatment with capsazepine, ondansetron or HC030031. In conscious rats, EOAR induced rapid and monophasic hypotensive and bradycardiac (phase 1) effects that were abolished by i.v. methylatropine. In endothelium-intact aortic rings, EOAR fully relaxed phenylephrine-induced contractions in a concentration-dependent manner. The present findings reveal that phase 1 of the bradycardiac and depressor responses induced by EOAR has a vago-vagal reflex origin resulting from the vagal pulmonary afferents stimulation. Such phenomenon appears not to involve the recruitment of C-fibre afferents expressing 5HT3 receptors or the two chemosensory ion channels TRPV1 and TRPA1 . Phase 2 hypotensive response appears resulting from a direct vasodilatory action.

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1-Nitro-2-phenylethane, the main constituent of the essential oil of Aniba canelilla, elicits a vago-vagal bradycardiac and depressor reflex in normotensive rats

Cited by 35 publications

References 26 publications

Cardiovascular effects of 1-nitro-2-phenylethane, the main constituent of the essential oil of Aniba canelilla, in spontaneously hypertensive rats

Cardiovascular effects of 1-nitro-2-phenylethane, the main constituent of the essential oil of Aniba canelilla, in spontaneously hypertensive rats

Perivagal antagonist treatment in rats selectively blocks the reflex and afferent responses of vagal lung C fibers to intravenous agonists

Linalool‐rich Rosewood Oil Induces Vago‐vagal Bradycardic and Depressor Reflex in Rats

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