1-Hydroxypyrene in human urine after exposure to coal tar and a coal tar derived product

Jongeneelen, F. J.; Anzion, R. B. M.; Leijdekkers, Ch.‐M.; Bos, R.P.; Henderson, P.Th.

doi:10.1007/bf00383545

Cited by 175 publications

(91 citation statements)

References 9 publications

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“…Among these, urinary metabolites 1-OHP and 2-naphthol have served as good biomonitoring markers (Jongeneelen et al, 1985;Kim et al, 1999). In our present study, the urinary 1-OHP and 2-naphthol concentration in both subjects were found to be similar to those in aircraft maintenance and shipyard workers Lee et al, 2001).…”

Section: Discussionsupporting

confidence: 71%

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Kim

Oh²,

Lee³

et al. 2004

Exp Mol Med

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Section: Discussionsupporting

confidence: 71%

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Kim

Oh²,

Lee³

et al. 2004

Exp Mol Med

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“…The analytic procedure to determine urinary 1-OHP levels using reverse-phase highperformance liquid chromatography with fluorescence detection has been described previously (16,24,25). Reported levels of 1-OHP are the sum of the free compounds and the sulfate and glucuronide conjugates.…”

Section: Methodsmentioning

confidence: 99%

A Urinary Metabolite of Phenanthrene as a Biomarker of Polycyclic Aromatic Hydrocarbon Metabolic Activation in Workers Exposed to Residual Oil Fly Ash

Kim

Hecht

Mukherjee³

et al. 2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Residual oil fly ash is a chemically complex combustion product containing a significant component of potentially carcinogenic transition metals and polycyclic aromatic hydrocarbons (PAH). Various biomarkers of PAH exposure have been investigated previously, most notably 1-hydroxypyrene (1-OHP), in urine. In this study, we assessed the utility of r -1,t -2,3,c -4-tetrahydroxy-1,2,3,4-tetrahydrophenanthrene (trans, anti-PheT), a metabolite of phenanthrene, to detect occupational PAH exposure. Urine samples collected across the workweek were analyzed for 1-OHP and trans, antiPheT in boilermakers (n = 20) exposed to residual oil fly ash. Median baseline urinary trans, anti-PheT concentrations were 0.50 Mg/g creatinine in current tobacco smokers and 0.39 Mg/g creatinine in nonsmokers. Median baseline urinary 1-OHP concentrations in smokers and nonsmokers were 0.31 and 0.13 Mg/g creatinine, respectively. To study further the effect of smoking exposure on the urinary PAH markers, urinary cotinine was used. Although urinary trans, anti-PheT and 1-OHP concentrations were correlated (Spearman r = 0.63; P < 0.001) for all subjects, the regression coefficient between log-transformed trans, antiPheT and log 1-OHP was statistically significant only for subjects with low levels of urinary cotinine or for nonsmokers. Each 1-unit increase in log 1-OHP was associated with a 0.77-unit increase (95% confidence interval, 0.45-1.09) in log trans, anti-PheT in subjects with low levels of urinary cotinine (P < 0.001). In these subjects, dichotomized occupational exposure status was a significant predictor of log trans, anti-PheT (P = 0.02) but not of log 1-OHP (P = 0.2). In conclusion, we found that urinary trans, anti-PheT was detected in levels comparable with 1-OHP in occupationally exposed workers, particularly nonsmokers. This study shows that urinary trans, antiPheT may be an effective biomarker of uptake and metabolic activation of PAHs.

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“…The level of 1-OHP in urine was determined using reverse-phase high performance liquid chromatography (HPLC) after a modification of the method of Jongeneelen et al 33) . Ten milliliters of thawed urine were adjusted to pH 5.0 with 1.0 N acetic acid, 10 ml of 0.1 M sodium acetate buffer (pH 5.0) was added and the mixture was enzymatically deconjuated by 25 µl of β-glucuronidase/ sulfatase (127,300 units/ml and 7,500 units/ml).…”

Section: Determination Of 1-hydroxypyrene In Urinementioning

confidence: 99%

Urinary 1‐Hydroxypyrene Level Relative to Vehicle Exhaust Exposure Mediated by Metabolic Enzyme Polymorphisms

Chuang

Chang

2007

Journal of Occupational Health

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Urinary 1-Hydroxypyrene Level Relative to Vehicle Exhaust Exposure Mediated by MetabolicEnzyme Polymorphisms: Chun-Yu CHUANG, et al. Department of Biomedical Engineering andEnvironmental Sciences, National Tsing-Hua University, Taiwan-Polycyclic aromatic hydrocarbons (PAH) are common air pollutants generated from incomplete combustion. The inhalation of exhaust fumes in urban areas has been suggested to be an additional contributing factor. This study investigated the influence of urban traffic exposure, personal lifestyle factors and metabolic enzyme polymorphisms on the urinary 1-hydroxypyrene (1-OHP) level, approximating exposure to PAH. With consents, 95 male taxi drivers exposed to vehicle exhaust in traffic and 75 male office employees received health interviews and provided urine samples. The results showed taxi drivers had higher urinary 1-OHP than the office employees (mean ± standard deviation were 0.17 ± 0.10 vs. 0.10 ± 0.07 µmol/mol creatinine, p<0.001). The average urinary 1-OHP level increased from 0.07 µmol/mol creatinine for non-smoking office employees to 0.17 µmol/mol creatinine for those who smoked more than 20 cigarettes daily. The values for taxi drivers with similar smoking statuses were 0.12 and 0.25 µmol/mol creatinine, respectively. Among non-smokers, taxi drivers still had higher 1-OHP level than office employees (0.12 ± 0.05 vs. 0.07 ± 0.03 µmol/mol creatinine). The subjects with the m1/m2 or m2/m2 genotype of CYP1A1 MspI or GSTM1 deficiency had significantly higher urinary 1-OHP levels than those with other CYP1A1 MspI and GSTM1 genotypes. Multivariate logistic regression analysis showed that taxi drivers (adjusted odds ratio (OR)=5.1, 95% confidence interval (CI)=1.1-13.6), smokers (OR=5.5, 95% CI=1.6-18.4) and subjects with the m1/m2 or m2/ m2 genotype of CYP1A1 MspI (OR=9.7, 95% CI=2.7-35.0) had elevated urinary 1-OHP (greater than the overall median value, 0.11 µmol/mol creatinine). The results of this study suggest smoking contributes to the elevated urinary 1-OHP levels in taxi drivers in addition to taxi driving, and the excess level contributed from traffic exhaust and smoke was regulated by the CYP1A1 MspI genotype. Traffic exhaust exposure, smoking and CYP1A1 MspI genotype contributed to the variation in levels of urinary 1-OHP excretion.

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1-Hydroxypyrene in human urine after exposure to coal tar and a coal tar derived product

Cited by 175 publications

References 9 publications

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

A Urinary Metabolite of Phenanthrene as a Biomarker of Polycyclic Aromatic Hydrocarbon Metabolic Activation in Workers Exposed to Residual Oil Fly Ash

Urinary 1‐Hydroxypyrene Level Relative to Vehicle Exhaust Exposure Mediated by Metabolic Enzyme Polymorphisms

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