1-Dimethylaminonaphthalene-5-sulfonyl hydrazine (dansyl hydrazine): A fluorometric reagent for carbonyl compounds

Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction

“…However, most carbonyl compounds are non-fluorescent or weakly fluorescent. In order to determine the carbonyl compounds fluorimetrically, several fluorescence derivatization reagents having a hydrazine group have been developed, e.g., HCPI [5], DBD-H [6] and dansyl hydrazine [7]. However, hydrazine reagents are unstable and should be prepared just before analysis.…”

The Identification of Carbonyl Compounds by Fluorescence: A Novel Carbonyl-Derivatizing Reagent

“…[1] This reaction was originally developed for the characterization of carbonyl compounds by melting points. On the other hand, the strong colour of the 2,4-dinitrophenylhydrazones (2) offer the possibility of their visual identification or UV/visible-spectroscopic detection and quantitative determination. This can be carried out simply, but the sensitivity for Scheme 1.…”

Tris(2,2′-bipyridine)ruthenium(III) Chemiluminescence Detection of Carbonyl Compounds with Methylmalonic Acid

“…Other substituted hydrazones are also used as analytical derivatives, and offer a basic potential for the development of new reagents. 2 The oximes are also classical derivatives of carbonyl compounds. Several O-substituted oximes have been employed analytically.…”

“…These results were superior to the previously published methods. [1][2][3] In conclusion, a sensitive detection method for carbonyl compounds was developed. The application of derivatization scheme using methylmalonic acid as the labeling reagent for carbonyl compounds has been successfully performed with HPLC using Ru(bpy)3 2+ ECL detection, and this method should prove to be useful for the selective and sensitive detection of compounds having carbonyl moiety such as dimethylketone, keto acids, ketosteroids.…”