“…Divinylsulfone (Figure 8.21), which converts primary amines into l,4-thiazane-1,1-dioxides, has been used in procedures to determine amino acids in serum [152]. The derivatization of carbonyl compounds with methylmalonic acid, and amines with diketene, to form species that produce light with tris(2 ,2' -bipyridi ne )ruthenium(llI) has also been demonstrated [153,154]. Although these procedures provide sensitive detection, the preliminary off-line derivatization of the target analytes increases the overall analysis time by 20-120min.…”