1,5-Diaminopentane production from xylooligosaccharides using metabolically engineered Corynebacterium glutamicum displaying beta-xylosidase on the cell surface

Imao, Kenta; Konishi, Rie; Kishida, Mayumi; Hirata, Yasushi; Segawa, Shota; Adachi, Naoko; Matsuura, Rena; Tsuge, Yota; Matsumoto, Takuya; Tanaka, Tsutomu; Kondo, Akihiko

doi:10.1016/j.biortech.2017.05.135

Cited by 37 publications

(21 citation statements)

References 32 publications

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“…7,61 A greener route to nylon-6,6 via bio-adipic acid was recently reported 1,62-64 and reflects growing impetus to find renewable pathways to polyamides. 14,15,18,64 To demonstrate the potential of (bio-based) glutaric acid as a polyamide precursor, we successfully synthesized nylon-6,5 from the novel bio-based glutaric acid and from the commercial petrochemical for comparison. The interfacial polymerization (the nylon "rope trick") ( Fig.…”

Section: The Overexpression Of the Permease Ncgl0464 Enables Almost Ementioning

confidence: 99%

“…At present, the bioplastics market grows between 20-100% per year and the production is expected to surpass six million tons in 2021. This trend explains the huge market potential assigned to bio-based chemicals, which can serve as building blocks for bioplastics, including dicarboxylic acids, 1-9 diamines [10][11][12][13][14][15][16][17][18][19] and diols. [20][21][22][23][24] Glutaric acid (1,5-pentanedioic acid) is such an attractive building block for polymers.…”

Section: Introductionmentioning

confidence: 99%

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A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineeredCorynebacterium glutamicum

et al. 2018

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Section: The Overexpression Of the Permease Ncgl0464 Enables Almost Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineeredCorynebacterium glutamicum

et al. 2018

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“…Adachi et al have produced 1.08 g/L medium of L-lysine from a cellobiose carbon source using a system in which β-glucosidase was displayed using PorC as an anchor protein (Adachi et al, 2013 ). In addition, Imao et al have reported the display of β-xylosidase on the cell surface using PorH as an anchor protein (Imao et al, 2017 ). In this expression system, xylooligosaccharides were used as a carbon source to produce 0.12 g/L medium of 1,5-diaminopentane (cadaverine).…”

Section: Surface-displayed Enzyme Expression In C Glutamicmentioning

confidence: 99%

Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application

Lee

Kim

2018

Front. Microbiol.

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Corynebacterium glutamicum, a soil-derived gram-positive actinobacterium, has been widely used for the production of biochemical molecules such as amino acids (i.e., L-glutamate and L-lysine), nucleic acids, alcohols, and organic acids. The metabolism of the bacterium has been engineered to increase the production of the target biochemical molecule, which requires a cytosolic enzyme expression. As recent demand for new proteinaceous biologics (such as antibodies, growth factors, and hormones) increase, C. glutamicum is attracting industrial interest as a recombinant protein expression host for therapeutic protein production due to the advantages such as low protease activity without endotoxin activity. In this review, we have summarized the recent studies on the heterologous expression of the recombinant protein in C. glutamicum for metabolic engineering, expansion of substrate availability, and recombinant protein secretion. We have also outlined the advances in genetic components such as promoters, surface anchoring systems, and secretory signal sequences in C. glutamicum for effective recombinant protein expression.

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“…Moreover, downstream purification processes of fermentation‐derived DAP and its polycondensation into a bionylon have been developed (Kim et al, ; Kind et al, ). Although a broad range of alternative substrates such as starch (Tateno et al, ), xylose (Buschke et al, , ), and xylooligosaccharides (Imao et al, ) have been used in DAP production, cellulosic materials have not yet been used.…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering to improve 1,5‐diaminopentane production from cellobiose using β‐glucosidase‐secreting Corynebacterium glutamicum

Matsuura

Kishida

Konishi

et al. 2019

Biotech & Bioengineering

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Microbial production of 1,5-diaminopentane (DAP) from renewable feedstock is a promising and sustainable approach for the production of polyamides. In this study, we constructed a β-glucosidase (BGL)-secreting Corynebacterium glutamicum and successfully used this strain to produce DAP from cellobiose and glucose. First, C. glutamicum was metabolically engineered to produce L-lysine (a direct precursor of DAP), followed by the coexpression of L-lysine decarboxylase and BGL derived from Escherichia coli and Thermobifida fusca YX (Tfu0937), respectively. This new engineered C. glutamicum strain produced 27 g/L of DAP from cellobiose in CGXII minimal medium using fed-batch cultivation. The yield of DAP was 0.43 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose), which is the highest yield reported to date. These results demonstrate the feasibility of DAP production from cellobiose or cellooligosaccharides using an engineered C. glutamicum strain.

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1,5-Diaminopentane production from xylooligosaccharides using metabolically engineered Corynebacterium glutamicum displaying beta-xylosidase on the cell surface

Cited by 37 publications

References 32 publications

A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineeredCorynebacterium glutamicum

A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineeredCorynebacterium glutamicum

Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application

Metabolic engineering to improve 1,5‐diaminopentane production from cellobiose using β‐glucosidase‐secreting Corynebacterium glutamicum

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