1,3-Propanediol production potential of Clostridium saccharobutylicum NRRL B-643

Güngörmüşler, Mine; Gönen, Çağdaş; Özdemir, Güven; Azbar, Nuri

doi:10.1016/j.nbt.2010.07.010

Cited by 37 publications

(17 citation statements)

References 24 publications

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“…Clostridium beijerinckii NRRL B-593 is an anaerobic mesophilic and rarely studied bacteria which also can grow on facultative anaerobic conditions [8]. The continuous fermentation process with this microorganism in which immobilized cell bioreactors are used have been found to be more resistant to washout at high dilution rates.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, higher production rates were observed in less hydraulic retention times (HRT). The objective of this work was to investigate the effects of HRT (16,12,8,6,4, and 2 h) and immobilization material (pumice stones and ceramic rings) on cell growth, glycerol utilization and 1,3-PDO production using immobilized bioreactor systems. By means of this promising technique, production rates of up to 30 g/L/h are feasible, which means an increase of 2.5-fold compared to processes with freely suspended cells.…”

Section: Introductionmentioning

confidence: 99%

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Continuous production of 1,3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture

Güngörmüşler

Gönen

Azbar

2011

Bioprocess Biosyst Eng

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The continuous production of 1,3-propanediol (1,3-PDO) was investigated with Clostridium beijerinckii NRRL B-593 using raw glycerol without purification obtained from a biodiesel production process. Ceramic rings and pumice stones were used for cell immobilization in a packed-bed bioreactor. For comparison purpose, a control bioreactor with suspended culture was also run. The effect of hydraulic retention time (HRT) on the production of 1,3-PDO in both immobilized and suspended bioreactors were also investigated. The study revealed that HRT is an important factor for both immobilized and suspended systems and a HRT of 2 h is the best one in terms of volumetric production rate (g 1,3-PDO/L/h). Furthermore, cell immobilization had also obvious benefits especially for the robustness and the reliability of the production. The results indicated that cell immobilization achieved a 2.5-fold higher productivity in comparison to suspended cell system. Based on our results, continuous production of 1,3-PDO with immobilized cells is an efficient method, and raw glycerol can be utilized without any pretreatment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Continuous production of 1,3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture

Güngörmüşler

Gönen

Azbar

2011

Bioprocess Biosyst Eng

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“…It was reported that Clostridium kluyveri could involve 4‐hydroxybutyrate‐CoA:CoA transferase, 4‐hydroxybutyrate dehydrogenase, and succinic semialdehyde dehydrogenase to form 4‐hydroxybutyryl‐CoA the precursor for 4‐hydroxybutyrate that proposed through a recombinant E coli strain. C saccharobutylicum NRRL B‐643 is considered a potent candidate for 1,3‐propanediol production from glycerol yielding 0.36 mol mol −1 , while C beijerinckii exhibited the highest yield of 0.54 mol mol −1 …”

Section: Discussionmentioning

confidence: 99%

Production of butanol and polyhydroxyalkanoate from industrial waste byClostridium beijerinckiiASU10

et al. 2019

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Summary This study demonstrated a biotechnological approach for simultaneous production of low‐cost H2, liquid biofuels, and polyhydroxyalkanoates (PHAs) by solventogenic bacterium (Clostridium beijerinckii) from renewable industrial wastes such as molasses and crude glycerol. C beijerinckii ASU10 (KF372577) exhibited considerable performance for hydrogen production of 5.1 ± 0.84 and 11 ± 0.44 mL H2 h−1 on glycerol and sugarcane molasses, respectively. The total acetone‐butanol‐ethanol (ABE) generation from glycerol and molasses was 9.334 ± 2.98 and 10.831 ± 4.1 g L−1, respectively. ABE productivity (g L−1 h−1) was 0.0486 and 0.0564 with a yield rate (g g−1) up to 0.508 and 0.493 from glycerol and molasses fermentation, respectively. The PHA yields from glycerol and sugarcane molasses were 84.37% and 37.97% of the dried bacterial biomass, respectively. Additionally, the ultrathin section of C beijerinckii ASU10 showed that PHA granules were accumulated more densely on glycerol than molasses. Gas chromatography–mass spectrometry (GC‐MS) analysis confirmed that the PHAs obtained from molasses fermentation included 3‐hydroxybutyrate (47.3%) and 3‐hydroxyoctanoate (52.7%) as the main constituents. Meanwhile, 3‐hydroxybutyrate represented the sole monomer of PHA produced from glycerol fermentation. This study demonstrated that C beijerinckii ASU10 (KF372577) is a potent strain for low‐cost PHA production depending on its high potential to produce high‐energy biofuel and other valuable compounds from utilization of organic waste materials.

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“…butyricum species only. Gungormusler et al [35] examined a batch fermentation of Cl. saccharobutylicum NRRL B-643 and achieved glycerol efficiency of 0.36 mol/mol.…”

Section: Isolation Of New Strainsmentioning

confidence: 99%

Isolation of New Strains of Bacteria Able to Synthesize 1,3-Propanediol from Glycerol

Szymanowska¹,

Drożdżyńska²,

Remszel³

2013

AiM

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The natural environment is inhabited by many species that exhibit very specific metabolic activities that may find industrial applications. The aim of the study was to select non-pathogenic cultures of bacteria of the genus Clostridium and lactic acid bacteria able to convert glycerol into 1,3-propanediol (1,3-PD). Another aim of this study was to identify the isolates that best produced 1,3-propanediol both from pure and crude glycerol. The most efficient strains identified (Cl. butyricum) were analysed on a bioreactor scale. The aim was to determine temperature conditions on the efficiency and duration of 1,3-PD synthesis. The species Clostridium were identified using amplification of the 16S rRNA coding sequence. A total of 123 isolates (of the genus Clostridium and lactic acid bacteria) were isolated; a vast majority of these were able to synthesize 1,3-PD. The best results were obtained for Cl. butyricum strain DSP1, which was isolated from the rumen of a cow fed with glycerol. The strain efficiency using pure glycerol on bioreactor scale 0.65 mol/mol of glycerol at a temperature of 38˚C and a constant pH of 7.0.

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1,3-Propanediol production potential of Clostridium saccharobutylicum NRRL B-643

Cited by 37 publications

References 24 publications

Continuous production of 1,3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture

Continuous production of 1,3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture

Production of butanol and polyhydroxyalkanoate from industrial waste byClostridium beijerinckiiASU10

Isolation of New Strains of Bacteria Able to Synthesize 1,3-Propanediol from Glycerol

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