1,3‐Propanediol production from glycerol by a newly isolated Trichococcus strain

Abstract

SummaryA coccal bacterium (strain ES5) was isolated from methanogenic bioreactor sludge with glycerol as the sole energy and carbon source. Strain ES5 fermented glycerol to 1,3‐propanediol as main product, and lactate, acetate and formate as minor products. The strain was phylogenetically closely related to Trichococcus flocculiformis; the rRNA gene sequence similarity was 99%. However, strain ES5 does not show the typical growth in chains of T. flocculiformis. Moreover, T. flocculiformis does not ferment glycerol. Strain ES5 used a variety of sugars for growth. With these substrates, lactate, acetate and formate were the main products, while 1,3‐propanediol was not formed. The optimum growth temperature of strain ES5 ranges from 30–37°C, but like several other Trichoccoccus strains, strain ES5 is able to grow at low temperature (< 10°C). Therefore, strain ES5 may be an appropriate catalyst for the biotechnological production of 1,3‐propanediol from glycerol at low ambient temperature.

Adaptation of Akkermansia muciniphila to the Oxic-Anoxic Interface of the Mucus Layer

“…To determine the production of metabolites, 1.5 ml of bacterial cultures was centrifuged and the supernatant was stored at Ϫ20°C. Levels of short-chain fatty acids (SCFAs) were determined by high-performance liquid chromatography (HPLC) as previously described (20). Total protein concentrations were determined by the use of a Pierce bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, IL, USA) and Qubit (Life Technologies, Eugene, OR, USA) according to the manufacturer's protocols.…”

Description of Trichococcus ilyis sp. nov. by combined physiological and in silico genome hybridization analyses

“…T to utilize a set of substrates had been previously tested (van Gelder et al, 2012). In this study, the following additional substrates were tested (at a concentration of 20 mM, unless otherwise mentioned): 5 Pa].…”

Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

“…Microbial glycerol fermentation is an interesting way to produce valuable compounds such as 1,3-propanediol (1,3-PDO), acetic acid, butyric acid, acetone, ethanol, 2,3-butanediol, lactic acid, Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH (Received September 6, 2016;Accepted February 28, 2017; J-STAGE Advance publication date: November 29, 2017) Sidnei C. Santos, 1,2,* Martin G. Liebensteiner, 1 Antonie H. van Gelder, 1 Mauricio R. Dimitrov, succinic acid, formate, or H 2 (Biebl et al, 1999;Ito et al, 2005;Kivistö et al, 2010;Wischral et al, 2016;Yang et al, 2007). Glycerol fermentation has been observed in species belonging to the genera Citrobacter, Clostridium, Enterobacter, Klebsiella, Propionibacterium, Propionispora and Trichococcus (Abou-Zeid et al, 2004;Biebl et al, 1999;Himmi et al, 2000;Johnson and Taconi, 2007;van Gelder et al, 2011). Glycerol may also be used as an electron donor for reduction processes such as sulfate reduction.…”