Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

Santos, Sidnei Cerqueira dos; Liebensteiner, Martin G.; Gelder, A.H. van; Dimitrov, Maurício R.; Almeida, Paulo Fernando de; Quintella, Cristina M.; Stams, Alfons Johannes Maria; Sánchez‐Andrea, Irene

doi:10.2323/jgam.2017.02.009

Cited by 11 publications

(5 citation statements)

References 57 publications

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“…Acetate accumulation coincided with methanogenic activity cease, which caused acetate concentration peaks up to 650 mg O2 L -1 . This result indicated that acetate was consumed by acetoclastic methanogens and that heterotrophic sulfate reducers were probably not consuming acetate to reduce sulfate as reported by other authors (Qatibi et al, 1998;Dinkel et al, 2010b;Postgate, 2013;Santos et al, 2017). In addition, and considering that around 15-20% of crude glycerol was slowly biodegradable, an average outlet acetate concentration of 510 mg COD•L -1 (Figure 2C) coupled to an outlet total COD concentration of around 700 mg COD•L -1 at the end of the UASB operation (Figure 2B) indicated that acidogenesis and acetogenesis were not severely affected by the pH shock.…”

Section: Assessment Of the Sulfidogenic Uasb Performancesupporting

confidence: 75%

“…Santos et al (2018) recently reported that the use of crude glycerol promotes sulfate-reducing communities growth while Mora et al (2018) also demonstrated that crude glycerol is a powerful organic source to promote sulfidogenesis under certain conditions. Crude glycerol use in sulfidogenic reactors has been described for recovering precious or valuable metals, for immobilizing heavy/toxic metals or simply to study and get knowledge about the competition between methanogens and sulfate reducers (Dinkel et al, 2010b;Bertolino et al, 2014;Santos et al, 2017). On the contrary, the use of crude glycerol in sulfidogenic UASB reactors towards elemental sulfur recovery is scarce.…”

Section: Introductionmentioning

confidence: 99%

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Influence of crude glycerol load and pH shocks on the granulation and microbial diversity of a sulfidogenic Upflow Anaerobic Sludge Blanket reactor

Mora

Lafuente

Gabriel

2020

Process Safety and Environmental Protection

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Section: Assessment Of the Sulfidogenic Uasb Performancesupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Influence of crude glycerol load and pH shocks on the granulation and microbial diversity of a sulfidogenic Upflow Anaerobic Sludge Blanket reactor

Mora

Lafuente

Gabriel

2020

Process Safety and Environmental Protection

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“…This work demonstrates the coupling of glycerol oxidation to dye reduction by consortium SCP under anoxic conditions. In earlier reports, the glycerol has been exploited for simultaneous sulfate reduction by microorganisms and electricity generation in a microbial fuel cell (MFC) [ 67 , 68 ]. The RB28 decolorization by consortium SCP seems to occur by reductases mediated cleavage of azo-bond under anoxic condition.…”

Section: Discussionmentioning

confidence: 99%

Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

2021

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Background A bacterial consortium SCP comprising three bacterial members, viz. Stenotrophomonas acidaminiphila APG1, Pseudomonas stutzeri APG2 and Cellulomonas sp. APG4 was developed for degradation of the mono-azo dye, Reactive Blue 28. The genomic analysis of each member of the SCP consortium was done to elucidate the catabolic potential and role of the individual organism in dye degradation. Results The genes for glycerol utilization were detected in the genomes of APG2 and APG4, which corroborated with their ability to grow on a minimal medium containing glycerol as the sole co-substrate. The genes for azoreductase were identified in the genomes of APG2 and APG4, while no such trait could be determined in APG1. In addition to co-substrate oxidation and dye reduction, several other cellular functions like chemotaxis, signal transduction, stress-tolerance, repair mechanisms, aromatic degradation, and copper tolerance associated with dye degradation were also annotated. A model for azo dye degradation is postulated, representing the predominant role of APG4 and APG2 in dye metabolism while suggesting an accessory role of APG1. Conclusions This exploratory study is the first-ever attempt to divulge the genetic basis of azo-dye co-metabolism by cross-genome comparisons and can be harnessed as an example for demonstrating microbial syntrophy.

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“…The goals of the experiment were 1) to evaluate the response of oligotrophic and eutrophic systems to carbon amendment, 2) to investigate the chemical effects and mineral products of SRB activity in these systems, and 3) to evaluate the potential of glycerol amendment to remediate acidic pit lakes and AMD systems via biosulfidogenesis. Glycerol has been previously reported to be an appropriate carbon source for sulfate-reducing bacteria ( Qatibi et al, 1991 ; Santos et al, 2018 ). In addition glycerol is non-toxic for the environment, easily available in the market and has lower price in comparison to organic substrates mentioned above.…”

Section: Introductionmentioning

confidence: 99%

Glycerol amendment enhances biosulfidogenesis in acid mine drainage-affected areas: An incubation column experiment

Ilin

Graaf

Yusta

et al. 2022

Front. Bioeng. Biotechnol.

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Microbial sulfate (SO42−) reduction in Acid Mine Drainage (AMD) environments can ameliorate the acidity and extreme metal concentrations by consumption of protons via the reduction of SO42− to hydrogen sulfide (H2S) and the concomitant precipitation of metals as metal sulfides. The activity of sulfate-reducing bacteria can be stimulated by the amendment of suitable organic carbon sources in these generally oligotrophic environments. Here, we used incubation columns (IC) as model systems to investigate the effect of glycerol amendment on the microbial community composition and its effect on the geochemistry of sediment and waters in AMD environments. The ICs were built with natural water and sediments from four distinct AMD-affected sites with different nutrient regimes: the oligotrophic Filón Centro and Guadiana acidic pit lakes, the Tintillo river (Huelva, Spain) and the eutrophic Brunita pit lake (Murcia, Spain). Physicochemical parameters were monitored during 18 months, and the microbial community composition was determined at the end of incubation through 16S rRNA gene amplicon sequencing. SEM-EDX analysis of sediments and suspended particulate matter was performed to investigate the microbially-induced mineral (neo)formation. Glycerol amendment strongly triggered biosulfidogenesis in all ICs, with pH increase and metal sulfide formation, but the effect was much more pronounced in the ICs from oligotrophic systems. Analysis of the microbial community composition at the end of the incubations showed that the SRB Desulfosporosinus was among the dominant taxa observed in all sulfidogenic columns, whereas the SRB Desulfurispora, Desulfovibrio and Acididesulfobacillus appeared to be more site-specific. Formation of Fe3+ and Al3+ (oxy)hydroxysulfates was observed during the initial phase of incubation together with increasing pH while formation of metal sulfides (predominantly, Zn, Fe and Cu sulfides) was observed after 1–5 months of incubation. Chemical analysis of the aqueous phase at the end of incubation showed almost complete removal of dissolved metals (Cu, Zn, Cd) in the amended ICs, while Fe and SO42− increased towards the water-sediment interface, likely as a result of the reductive dissolution of Fe(III) minerals enhanced by Fe-reducing bacteria. The combined geochemical and microbiological analyses further establish the link between biosulfidogenesis and natural attenuation through metal sulfide formation and proton consumption.

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Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

Cited by 11 publications

References 57 publications

Influence of crude glycerol load and pH shocks on the granulation and microbial diversity of a sulfidogenic Upflow Anaerobic Sludge Blanket reactor

Influence of crude glycerol load and pH shocks on the granulation and microbial diversity of a sulfidogenic Upflow Anaerobic Sludge Blanket reactor

Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

Glycerol amendment enhances biosulfidogenesis in acid mine drainage-affected areas: An incubation column experiment

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